TY  - JOUR
AU  - Prijović, Mladen
AU  - Nikolić, Bogdan
AU  - Dragićević, Ivana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Giba, Zlatko
AU  - Jovanović, Vladan
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1249
AB  - Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić & Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.
PB  - Serbian Biological Socitey
T2  - Archives of Biological Sciences
T1  - Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: potential use as a bioherbicide
EP  - 14
IS  - 1
SP  - 5
VL  - 76
DO  - 10.2298/ABS231107041P
ER  - 

@article{
author = "Prijović, Mladen and Nikolić, Bogdan and Dragićević, Ivana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Giba, Zlatko and Jovanović, Vladan",
year = "2023",
abstract = "Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić & Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.",
publisher = "Serbian Biological Socitey",
journal = "Archives of Biological Sciences",
title = "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: potential use as a bioherbicide",
pages = "14-5",
number = "1",
volume = "76",
doi = "10.2298/ABS231107041P"
}

Prijović, M., Nikolić, B., Dragićević, I., Nestorović Živković, J., Dmitrović, S., Giba, Z.,& Jovanović, V.. (2023). Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: potential use as a bioherbicide. in Archives of Biological Sciences
Serbian Biological Socitey., 76(1), 5-14.
https://doi.org/10.2298/ABS231107041P

Prijović M, Nikolić B, Dragićević I, Nestorović Živković J, Dmitrović S, Giba Z, Jovanović V. Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: potential use as a bioherbicide. in Archives of Biological Sciences. 2023;76(1):5-14.
doi:10.2298/ABS231107041P .

Prijović, Mladen, Nikolić, Bogdan, Dragićević, Ivana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Giba, Zlatko, Jovanović, Vladan, "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: potential use as a bioherbicide" in Archives of Biological Sciences, 76, no. 1 (2023):5-14,
https://doi.org/10.2298/ABS231107041P . .

TY  - CONF
AU  - Anđelković, Ana
AU  - Šikuljak, Danijela
AU  - Rajković, Miloš
AU  - Popović, Slađana
AU  - Marisavljević, Dragana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1193
AB  - Režim upravljanja i mere suzbijanja korova igraju ključnu ulogu u brojnosti i
diverzitetu korovske flore vinograda. U brojnim istraživanjima proučavan je uticaj mera
suzbijanja korova u vinogradima, dok su istraživanja uticaja uslova staništa na diverzitet
korovske flore vinograda retka na globalnom nivou i gotovo nepostojeća za područje
jugoistočne Evrope. Imajući u vidu blizinu istraživanih lokaliteta i slične mere suzbijanja
korova koje se sprovode u tri istraživana vinograda, cilj u ovom radu bio je da se pokaže da li
razlike u karakteristikama staništa, ali i lokalne razlike u okviru samih vinograda utiču na
sastav korovske flore vinograda.
Terenska istraživanja su sprovedena u okviru tri vinograda na području Vršačkog
vinogorja, pri čemu su u svakom od tri vinograda nasumično odabrane četiri celine u okviru
kojih su postavljena po četiri polja od 1 m2 (dva na ivici redova i dva u središtu redova).
Korovi su determinisani do nivoa vrste i utvrđivana je njihova brojnost u svakom od 48 polja.
Multivarijaciona analiza je korišćena da bi se pokazalo kako floristički sastav korova varira u
zavisnosti od parametara specifičnih za svaki od lokaliteta, ali i između celina unutar
istraživanih lokaliteta. Dve zasebne kanonijske korespodentne analize (CCA), analiza glavnih
komponenti (PCA) i tri analize redundantnosti (RDA) su urađene u CANOCO 5.0 softveru.
CCA je pokazala statistički značajno razdvajanje u sastavu korovske flore između tri
istraživana lokaliteta, odnosno vinograda. PCA i RDA su ilustrovale snažan uticaj uslova
specifičnih za lokalitet (širina reda, tip zemljišta i specifične predeone karakteristike
lokaliteta) na prisustvo različitih životnih formi i fenoloških grupa korova. Takođe, CCA je
pokazala da uslovi na nivou istraživanih celina u okviru svakog od lokaliteta (odnosno u
okviru pojedinačnih vinograda) takođe rezultuju u razlikama u sastavu korovske flore, što
dovodi do dominantnosti određenih korovskih vrsta u različitim delovima svakog od
istraživanih vinograda.
PB  - Društvo za zaštitu bilja
C3  - Zbornik XVII simpozijuma o zaštiti bilja, 27-30. novembar 2023, Zlatibor, Srbija
T1  - Uticaj lokalnih karakteristika staništa na sastav korovske flore u vinogradima Vršačkog vinogorja
SP  - 24
ER  - 

@conference{
author = "Anđelković, Ana and Šikuljak, Danijela and Rajković, Miloš and Popović, Slađana and Marisavljević, Dragana",
year = "2023",
abstract = "Režim upravljanja i mere suzbijanja korova igraju ključnu ulogu u brojnosti i
diverzitetu korovske flore vinograda. U brojnim istraživanjima proučavan je uticaj mera
suzbijanja korova u vinogradima, dok su istraživanja uticaja uslova staništa na diverzitet
korovske flore vinograda retka na globalnom nivou i gotovo nepostojeća za područje
jugoistočne Evrope. Imajući u vidu blizinu istraživanih lokaliteta i slične mere suzbijanja
korova koje se sprovode u tri istraživana vinograda, cilj u ovom radu bio je da se pokaže da li
razlike u karakteristikama staništa, ali i lokalne razlike u okviru samih vinograda utiču na
sastav korovske flore vinograda.
Terenska istraživanja su sprovedena u okviru tri vinograda na području Vršačkog
vinogorja, pri čemu su u svakom od tri vinograda nasumično odabrane četiri celine u okviru
kojih su postavljena po četiri polja od 1 m2 (dva na ivici redova i dva u središtu redova).
Korovi su determinisani do nivoa vrste i utvrđivana je njihova brojnost u svakom od 48 polja.
Multivarijaciona analiza je korišćena da bi se pokazalo kako floristički sastav korova varira u
zavisnosti od parametara specifičnih za svaki od lokaliteta, ali i između celina unutar
istraživanih lokaliteta. Dve zasebne kanonijske korespodentne analize (CCA), analiza glavnih
komponenti (PCA) i tri analize redundantnosti (RDA) su urađene u CANOCO 5.0 softveru.
CCA je pokazala statistički značajno razdvajanje u sastavu korovske flore između tri
istraživana lokaliteta, odnosno vinograda. PCA i RDA su ilustrovale snažan uticaj uslova
specifičnih za lokalitet (širina reda, tip zemljišta i specifične predeone karakteristike
lokaliteta) na prisustvo različitih životnih formi i fenoloških grupa korova. Takođe, CCA je
pokazala da uslovi na nivou istraživanih celina u okviru svakog od lokaliteta (odnosno u
okviru pojedinačnih vinograda) takođe rezultuju u razlikama u sastavu korovske flore, što
dovodi do dominantnosti određenih korovskih vrsta u različitim delovima svakog od
istraživanih vinograda.",
publisher = "Društvo za zaštitu bilja",
journal = "Zbornik XVII simpozijuma o zaštiti bilja, 27-30. novembar 2023, Zlatibor, Srbija",
title = "Uticaj lokalnih karakteristika staništa na sastav korovske flore u vinogradima Vršačkog vinogorja",
pages = "24"
}

Anđelković, A., Šikuljak, D., Rajković, M., Popović, S.,& Marisavljević, D.. (2023). Uticaj lokalnih karakteristika staništa na sastav korovske flore u vinogradima Vršačkog vinogorja. in Zbornik XVII simpozijuma o zaštiti bilja, 27-30. novembar 2023, Zlatibor, Srbija
Društvo za zaštitu bilja., 24.

Anđelković A, Šikuljak D, Rajković M, Popović S, Marisavljević D. Uticaj lokalnih karakteristika staništa na sastav korovske flore u vinogradima Vršačkog vinogorja. in Zbornik XVII simpozijuma o zaštiti bilja, 27-30. novembar 2023, Zlatibor, Srbija. 2023;:24..

Anđelković, Ana, Šikuljak, Danijela, Rajković, Miloš, Popović, Slađana, Marisavljević, Dragana, "Uticaj lokalnih karakteristika staništa na sastav korovske flore u vinogradima Vršačkog vinogorja" in Zbornik XVII simpozijuma o zaštiti bilja, 27-30. novembar 2023, Zlatibor, Srbija (2023):24.

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1137
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1138
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
IS  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8, 2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8).
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8).
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023),
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Vrandečić, Karolina
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/875
AB  - Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia
EP  - 176
IS  - 1
SP  - 159
VL  - 55
DO  - 10.2298/gensr2301159i
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Blagojević, Milan and Bagi, Ferenc and Vrandečić, Karolina and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia",
pages = "176-159",
number = "1",
volume = "55",
doi = "10.2298/gensr2301159i"
}

Iličić, R., Jelušić, A., Blagojević, M., Bagi, F., Vrandečić, K., Stanković, S.,& Popović Milovanović, T.. (2023). Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika
Serbian Genetics Society., 55(1), 159-176.
https://doi.org/10.2298/gensr2301159i

Iličić R, Jelušić A, Blagojević M, Bagi F, Vrandečić K, Stanković S, Popović Milovanović T. Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika. 2023;55(1):159-176.
doi:10.2298/gensr2301159i .

Iličić, Renata, Jelušić, Aleksandra, Blagojević, Milan, Bagi, Ferenc, Vrandečić, Karolina, Stanković, Slaviša, Popović Milovanović, Tatjana, "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia" in Genetika, 55, no. 1 (2023):159-176,
https://doi.org/10.2298/gensr2301159i . .

TY  - JOUR
AU  - Ranković, Tamara
AU  - Nikolić, Ivan
AU  - Berić, Tanja
AU  - Popović Milovanović, Tatjana
AU  - Lozo, Jelena
AU  - Medić, Olja
AU  - Stanković, Slaviša
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/713
AB  - Members of the Pseudomonas syringae species complex are heterogeneous bacteria that are the most abundant bacterial plant pathogens in the plant phyllosphere, with strong abilities to exist on and infect different plant hosts and survive in/outside agroecosystems. In this study, the draft genome sequences of two pathogenic P. syringae pv. aptata strains with different in planta virulence capacities isolated from the phyllosphere of infected sugar beet were analyzed to evaluate putative features of survival strategies and to determine the pathogenic potential of the strains. The draft genomes of P. syringae pv. aptata strains P16 and P21 are 5,974,057 bp and 6,353,752 bp in size, have GC contents of 59.03% and 58.77%, respectively, and contain 3,439 and 3,536 protein-coding sequences, respectively. For both average nucleotide identity and pangenome analysis, P16 and P21 largely clustered with other pv. aptata strains from the same isolation source. We found differences in the repertoire of effectors of the type III secretion system among all 102 selected strains, suggesting that the type III secretion system is a critical factor in the different virulent phenotypes of P. syringae pv. aptata. During genome analysis of the highly virulent strain P21, we discovered genes for T3SS effectors (AvrRpm1, HopAW1, and HopAU1) that were not previously found in genomes of P. syringae pv. aptata. We also identified coding sequences for pantothenate kinase, VapC endonuclease, phospholipase, and pectate lyase in both genomes, which may represent novel effectors of the type III secretion system. IMPORTANCE Genome analysis has an enormous effect on understanding the life strategies of plant pathogens. Comparing similarities with pathogens involved in other epidemics could elucidate the pathogen life cycle when a new outbreak happens. This study represents the first in-depth genome analysis of Pseudomonas syringae pv. aptata, the causative agent of leaf spot disease of sugar beet. Despite the increasing number of disease reports in recent years worldwide, there is still a lack of information about the genomic features, epidemiology, and pathogenic life strategies of this particular pathogen. Our findings provide advances in disease etiology (especially T3SS effector repertoire) and elucidate the role of environmental adaptations required for prevalence in the pathobiome of the sugar beet. From the perspective of the very heterogeneous P. syringae species complex, this type of analysis has specific importance in reporting the characteristics of individual strains.
PB  - American Society for Microbiology
T2  - MICROBIOLOGY SPECTRUM
T1  - Genome Analysis of Two Pseudomonas syringae pv. aptata Strains with Different Virulence Capacity Isolated from Sugar Beet: Features of Successful Pathogenicity in the Phyllosphere Microbiome
IS  - 2
VL  - 11
DO  - 10.1128/spectrum.03598-22
ER  - 

@article{
author = "Ranković, Tamara and Nikolić, Ivan and Berić, Tanja and Popović Milovanović, Tatjana and Lozo, Jelena and Medić, Olja and Stanković, Slaviša",
year = "2023",
abstract = "Members of the Pseudomonas syringae species complex are heterogeneous bacteria that are the most abundant bacterial plant pathogens in the plant phyllosphere, with strong abilities to exist on and infect different plant hosts and survive in/outside agroecosystems. In this study, the draft genome sequences of two pathogenic P. syringae pv. aptata strains with different in planta virulence capacities isolated from the phyllosphere of infected sugar beet were analyzed to evaluate putative features of survival strategies and to determine the pathogenic potential of the strains. The draft genomes of P. syringae pv. aptata strains P16 and P21 are 5,974,057 bp and 6,353,752 bp in size, have GC contents of 59.03% and 58.77%, respectively, and contain 3,439 and 3,536 protein-coding sequences, respectively. For both average nucleotide identity and pangenome analysis, P16 and P21 largely clustered with other pv. aptata strains from the same isolation source. We found differences in the repertoire of effectors of the type III secretion system among all 102 selected strains, suggesting that the type III secretion system is a critical factor in the different virulent phenotypes of P. syringae pv. aptata. During genome analysis of the highly virulent strain P21, we discovered genes for T3SS effectors (AvrRpm1, HopAW1, and HopAU1) that were not previously found in genomes of P. syringae pv. aptata. We also identified coding sequences for pantothenate kinase, VapC endonuclease, phospholipase, and pectate lyase in both genomes, which may represent novel effectors of the type III secretion system. IMPORTANCE Genome analysis has an enormous effect on understanding the life strategies of plant pathogens. Comparing similarities with pathogens involved in other epidemics could elucidate the pathogen life cycle when a new outbreak happens. This study represents the first in-depth genome analysis of Pseudomonas syringae pv. aptata, the causative agent of leaf spot disease of sugar beet. Despite the increasing number of disease reports in recent years worldwide, there is still a lack of information about the genomic features, epidemiology, and pathogenic life strategies of this particular pathogen. Our findings provide advances in disease etiology (especially T3SS effector repertoire) and elucidate the role of environmental adaptations required for prevalence in the pathobiome of the sugar beet. From the perspective of the very heterogeneous P. syringae species complex, this type of analysis has specific importance in reporting the characteristics of individual strains.",
publisher = "American Society for Microbiology",
journal = "MICROBIOLOGY SPECTRUM",
title = "Genome Analysis of Two Pseudomonas syringae pv. aptata Strains with Different Virulence Capacity Isolated from Sugar Beet: Features of Successful Pathogenicity in the Phyllosphere Microbiome",
number = "2",
volume = "11",
doi = "10.1128/spectrum.03598-22"
}

Ranković, T., Nikolić, I., Berić, T., Popović Milovanović, T., Lozo, J., Medić, O.,& Stanković, S.. (2023). Genome Analysis of Two Pseudomonas syringae pv. aptata Strains with Different Virulence Capacity Isolated from Sugar Beet: Features of Successful Pathogenicity in the Phyllosphere Microbiome. in MICROBIOLOGY SPECTRUM
American Society for Microbiology., 11(2).
https://doi.org/10.1128/spectrum.03598-22

Ranković T, Nikolić I, Berić T, Popović Milovanović T, Lozo J, Medić O, Stanković S. Genome Analysis of Two Pseudomonas syringae pv. aptata Strains with Different Virulence Capacity Isolated from Sugar Beet: Features of Successful Pathogenicity in the Phyllosphere Microbiome. in MICROBIOLOGY SPECTRUM. 2023;11(2).
doi:10.1128/spectrum.03598-22 .

Ranković, Tamara, Nikolić, Ivan, Berić, Tanja, Popović Milovanović, Tatjana, Lozo, Jelena, Medić, Olja, Stanković, Slaviša, "Genome Analysis of Two Pseudomonas syringae pv. aptata Strains with Different Virulence Capacity Isolated from Sugar Beet: Features of Successful Pathogenicity in the Phyllosphere Microbiome" in MICROBIOLOGY SPECTRUM, 11, no. 2 (2023),
https://doi.org/10.1128/spectrum.03598-22 . .

TY  - JOUR
AU  - Popović, Slađana
AU  - Nikolić, Nataša
AU  - Pećić, Marija
AU  - Anđelković, Ana
AU  - Subakov Simic, Gordana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/707
AB  - Many show caves are vulnerable to various disturbances, meaning that conservation of such habitats, which would include monitoring of their ecological parameters and lampenfora (a growing problem worldwide), should be a priority. For the frst time in Serbia, lampenfora was monitored continously for 5 years (2016–2020), three times per year during the tourist season, in the Lazar Cave. Artifcial light created favorable conditions for the proliferation of phototrophic microorganisms that were developed not only as epiliths, but also endoliths, which poses a greater danger for cave substratum and structures. Although a higher diversity in general was found in Cyanobacteria (coccoid forms mostly), Chlorophyta were more widespread and abundant in samples, among which Chlorella, Stichococcus bacillaris, and Klebsormidium faccidum stood out. Chlorella is one of the genera making lampenfora dangerous, as it can switch from an autotrophic to a mixotrophic, and fnally to a heterotrophic lifestyle. The mosses protonema and mosses itself were also present. Even though the cave is closed for 6 months every year, lampenfora “legacy” always persisted on all sites from the previous year, spreading further over the years. Measured parameters (temperature, relative air humidity, light intensity, substrate pH, and substratum moisture), primary production, and bioflm parameters showed yearly, seasonal, or sampling site variations. Statistical analyses were used to examine the efect of the sampling year, the season, and sampling site on the selected measured parameters, while multivariate analyses were performed with taxa in relation to year, season, site, and main ecological parameters.
PB  - Springer
T2  - GEOHERITAGE
T1  - First Report on a 5-Year Monitoring of Lampenflora in a Famous Show Cave in Serbia
IS  - 1
VL  - 15
DO  - 10.1007/s12371-022-00771-z
ER  - 

@article{
author = "Popović, Slađana and Nikolić, Nataša and Pećić, Marija and Anđelković, Ana and Subakov Simic, Gordana",
year = "2023",
abstract = "Many show caves are vulnerable to various disturbances, meaning that conservation of such habitats, which would include monitoring of their ecological parameters and lampenfora (a growing problem worldwide), should be a priority. For the frst time in Serbia, lampenfora was monitored continously for 5 years (2016–2020), three times per year during the tourist season, in the Lazar Cave. Artifcial light created favorable conditions for the proliferation of phototrophic microorganisms that were developed not only as epiliths, but also endoliths, which poses a greater danger for cave substratum and structures. Although a higher diversity in general was found in Cyanobacteria (coccoid forms mostly), Chlorophyta were more widespread and abundant in samples, among which Chlorella, Stichococcus bacillaris, and Klebsormidium faccidum stood out. Chlorella is one of the genera making lampenfora dangerous, as it can switch from an autotrophic to a mixotrophic, and fnally to a heterotrophic lifestyle. The mosses protonema and mosses itself were also present. Even though the cave is closed for 6 months every year, lampenfora “legacy” always persisted on all sites from the previous year, spreading further over the years. Measured parameters (temperature, relative air humidity, light intensity, substrate pH, and substratum moisture), primary production, and bioflm parameters showed yearly, seasonal, or sampling site variations. Statistical analyses were used to examine the efect of the sampling year, the season, and sampling site on the selected measured parameters, while multivariate analyses were performed with taxa in relation to year, season, site, and main ecological parameters.",
publisher = "Springer",
journal = "GEOHERITAGE",
title = "First Report on a 5-Year Monitoring of Lampenflora in a Famous Show Cave in Serbia",
number = "1",
volume = "15",
doi = "10.1007/s12371-022-00771-z"
}

Popović, S., Nikolić, N., Pećić, M., Anđelković, A.,& Subakov Simic, G.. (2023). First Report on a 5-Year Monitoring of Lampenflora in a Famous Show Cave in Serbia. in GEOHERITAGE
Springer., 15(1).
https://doi.org/10.1007/s12371-022-00771-z

Popović S, Nikolić N, Pećić M, Anđelković A, Subakov Simic G. First Report on a 5-Year Monitoring of Lampenflora in a Famous Show Cave in Serbia. in GEOHERITAGE. 2023;15(1).
doi:10.1007/s12371-022-00771-z .

Popović, Slađana, Nikolić, Nataša, Pećić, Marija, Anđelković, Ana, Subakov Simic, Gordana, "First Report on a 5-Year Monitoring of Lampenflora in a Famous Show Cave in Serbia" in GEOHERITAGE, 15, no. 1 (2023),
https://doi.org/10.1007/s12371-022-00771-z . .

TY  - JOUR
AU  - Anđelković, Ana
AU  - Pavlovic, Danijela
AU  - Marisavljević, Dragana
AU  - Živković, Milica
AU  - Novković, Maja
AU  - Popović, Slađana
AU  - Cvijanović, Dušanka
AU  - Radulović, Snežana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/728
AB  - Riparian areas experience strong invasion pressures worldwide and represent important points of spread for invasive alien plants (IAPs) in the European mainland. The Danube Basin is a well-known point of high plant invasion levels. Given that the middle part of the Danube Basin is critically understudied and the general lack of data for Serbia, the study aimed to provide an insight into the spatial patterns of plant invasions in the riparian areas of Serbia (Middle Danube Basin area). A total of 250 field sites, distributed along 39 rivers (nine catchment areas) and six canal sections, were studied during a four-year period (2013–2016) for the presence and abundance of IAPs. At the landscape scale, we studied distribution patterns of IAPs, differences in invasion levels in different catchment areas and between rivers and canals. At the local scale, we investigated how the proximity to roads/railway lines, housing areas, different land-use types (primarily agriculture), and dominant vegetation on site related to invasion patterns. Of the 26 studied IAPs, those with a well-known weedy behavior, long history of cultivation and strong affinity for riparian areas prevailed in the study area. Riparian zones of the Danube catchment exhibited the highest invasion levels in terms of IAPs richness and abundance, followed by the catchment areas of the Timok, Sava and Zapadna Morava rivers. Surprisingly, the Danube-Tisa-Danube canal network had the lowest invasion level. At the local scale, agriculture in proximity of the field site and dominant vegetation on site were observed as significant predictors of the invasion level. On the other hand, proximity to roads/railway lines and housing areas was not related to the invasion level. Finally, our study provides the first systematic overview of IAPs’ distribution data for riparian areas of the Middle Danube Basin in Serbia, which could provide a basis for long-term monitoring of IAPs and development of future management plans.
PB  - Pensoft Publishers
T2  - NeoBiota
T1  - Plant invasions in riparian areas of the Middle Danube Basin in Serbia
IS  - 23
VL  - 71
DO  - 10.3897/neobiota.71.69716
ER  - 

@article{
author = "Anđelković, Ana and Pavlovic, Danijela and Marisavljević, Dragana and Živković, Milica and Novković, Maja and Popović, Slađana and Cvijanović, Dušanka and Radulović, Snežana",
year = "2022",
abstract = "Riparian areas experience strong invasion pressures worldwide and represent important points of spread for invasive alien plants (IAPs) in the European mainland. The Danube Basin is a well-known point of high plant invasion levels. Given that the middle part of the Danube Basin is critically understudied and the general lack of data for Serbia, the study aimed to provide an insight into the spatial patterns of plant invasions in the riparian areas of Serbia (Middle Danube Basin area). A total of 250 field sites, distributed along 39 rivers (nine catchment areas) and six canal sections, were studied during a four-year period (2013–2016) for the presence and abundance of IAPs. At the landscape scale, we studied distribution patterns of IAPs, differences in invasion levels in different catchment areas and between rivers and canals. At the local scale, we investigated how the proximity to roads/railway lines, housing areas, different land-use types (primarily agriculture), and dominant vegetation on site related to invasion patterns. Of the 26 studied IAPs, those with a well-known weedy behavior, long history of cultivation and strong affinity for riparian areas prevailed in the study area. Riparian zones of the Danube catchment exhibited the highest invasion levels in terms of IAPs richness and abundance, followed by the catchment areas of the Timok, Sava and Zapadna Morava rivers. Surprisingly, the Danube-Tisa-Danube canal network had the lowest invasion level. At the local scale, agriculture in proximity of the field site and dominant vegetation on site were observed as significant predictors of the invasion level. On the other hand, proximity to roads/railway lines and housing areas was not related to the invasion level. Finally, our study provides the first systematic overview of IAPs’ distribution data for riparian areas of the Middle Danube Basin in Serbia, which could provide a basis for long-term monitoring of IAPs and development of future management plans.",
publisher = "Pensoft Publishers",
journal = "NeoBiota",
title = "Plant invasions in riparian areas of the Middle Danube Basin in Serbia",
number = "23",
volume = "71",
doi = "10.3897/neobiota.71.69716"
}

Anđelković, A., Pavlovic, D., Marisavljević, D., Živković, M., Novković, M., Popović, S., Cvijanović, D.,& Radulović, S.. (2022). Plant invasions in riparian areas of the Middle Danube Basin in Serbia. in NeoBiota
Pensoft Publishers., 71(23).
https://doi.org/10.3897/neobiota.71.69716

Anđelković A, Pavlovic D, Marisavljević D, Živković M, Novković M, Popović S, Cvijanović D, Radulović S. Plant invasions in riparian areas of the Middle Danube Basin in Serbia. in NeoBiota. 2022;71(23).
doi:10.3897/neobiota.71.69716 .

Anđelković, Ana, Pavlovic, Danijela, Marisavljević, Dragana, Živković, Milica, Novković, Maja, Popović, Slađana, Cvijanović, Dušanka, Radulović, Snežana, "Plant invasions in riparian areas of the Middle Danube Basin in Serbia" in NeoBiota, 71, no. 23 (2022),
https://doi.org/10.3897/neobiota.71.69716 . .

TY  - JOUR
AU  - Popović, Slađana
AU  - Nikolić, Nataša
AU  - Pećić, Marija
AU  - Anđelković, Ana
AU  - Subakov SImić, Gordana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/972
AB  - Nowadays, caves are increasingly being transformed into tourist attractions by constructing paths, electricity, water infrastructure, and the introduction of light, thereby becoming affected by various disturbances that alter their inherent equilibrium. Conservation of these sensitive environments should be a priority from the moment of their opening. All parameters, including the potential changes around artificial lights, such as lampenflora, should be monitored. Lampenflora in show caves in Serbia has been done for several years in the Lazar and Resava caves, of which the Lazar cave was monitored for over 6 years. This work aimed to compare lampenflora of these two caves, considering samples taken in the year 2021 at the beginning and the end of the official tourist season. Artificial light created favorable conditions for the proliferation of phototrophs, and lampenflora developed in both caves. Considering the community type, epiliths and endoliths were present in both localities. The difference is that endoliths in the Lazar Cave covered more significant areas of substratum, constantly spreading, thus posing a more significant threat to cave substratum and structures. One of the problematic genera was Chlorella, which can switch from an autotrophic to a mixotrophic, and finally to a heterotrophic lifestyle, thus making lampenflora dangerous and hard to deal with. Lampenflora in the Lazar Cave was developed over a large area of the cave substratum, while in Resava Cave, it was usually localized near artificial lights. When caves were compared based on phototrophic microorganism composition, using multivariate analysis, all morphological groups of Cyanobacteria (coccoid, simple trichal, and heterocystous) were more related to the Resava cave and Chlorophyta and Bacillariophyta to the Lazar Cave (based on diversity and number of sampling sites on which they are found). In the Lazar and Resava cave, Cyanobacteria dominated at the beginning of the tourist season and Chlorophyta at the end. Mosses were present, too, with the difference that the moss protonema was more characteristic of Lazar Cave in many places. In contrast, developed mosses were frequently found in Resava Cave near reflectors. In addition to the analysis of phototrophic microorganisms, ecological parameters, primary production, and biofilm parameters were measured, showing seasonal or sampling site variations. We hope that monitoring will be performed more regularly in the future and that it will include a more significant number of show caves for their conservation.
T2  - ARPHA Conference Abstracts
T1  - Comparative analysis of lampenflora in two show caves in Serbia
DO  - 10.3897/aca.5.e87188
ER  - 

@article{
author = "Popović, Slađana and Nikolić, Nataša and Pećić, Marija and Anđelković, Ana and Subakov SImić, Gordana",
year = "2022",
abstract = "Nowadays, caves are increasingly being transformed into tourist attractions by constructing paths, electricity, water infrastructure, and the introduction of light, thereby becoming affected by various disturbances that alter their inherent equilibrium. Conservation of these sensitive environments should be a priority from the moment of their opening. All parameters, including the potential changes around artificial lights, such as lampenflora, should be monitored. Lampenflora in show caves in Serbia has been done for several years in the Lazar and Resava caves, of which the Lazar cave was monitored for over 6 years. This work aimed to compare lampenflora of these two caves, considering samples taken in the year 2021 at the beginning and the end of the official tourist season. Artificial light created favorable conditions for the proliferation of phototrophs, and lampenflora developed in both caves. Considering the community type, epiliths and endoliths were present in both localities. The difference is that endoliths in the Lazar Cave covered more significant areas of substratum, constantly spreading, thus posing a more significant threat to cave substratum and structures. One of the problematic genera was Chlorella, which can switch from an autotrophic to a mixotrophic, and finally to a heterotrophic lifestyle, thus making lampenflora dangerous and hard to deal with. Lampenflora in the Lazar Cave was developed over a large area of the cave substratum, while in Resava Cave, it was usually localized near artificial lights. When caves were compared based on phototrophic microorganism composition, using multivariate analysis, all morphological groups of Cyanobacteria (coccoid, simple trichal, and heterocystous) were more related to the Resava cave and Chlorophyta and Bacillariophyta to the Lazar Cave (based on diversity and number of sampling sites on which they are found). In the Lazar and Resava cave, Cyanobacteria dominated at the beginning of the tourist season and Chlorophyta at the end. Mosses were present, too, with the difference that the moss protonema was more characteristic of Lazar Cave in many places. In contrast, developed mosses were frequently found in Resava Cave near reflectors. In addition to the analysis of phototrophic microorganisms, ecological parameters, primary production, and biofilm parameters were measured, showing seasonal or sampling site variations. We hope that monitoring will be performed more regularly in the future and that it will include a more significant number of show caves for their conservation.",
journal = "ARPHA Conference Abstracts",
title = "Comparative analysis of lampenflora in two show caves in Serbia",
doi = "10.3897/aca.5.e87188"
}

Popović, S., Nikolić, N., Pećić, M., Anđelković, A.,& Subakov SImić, G.. (2022). Comparative analysis of lampenflora in two show caves in Serbia. in ARPHA Conference Abstracts.
https://doi.org/10.3897/aca.5.e87188

Popović S, Nikolić N, Pećić M, Anđelković A, Subakov SImić G. Comparative analysis of lampenflora in two show caves in Serbia. in ARPHA Conference Abstracts. 2022;.
doi:10.3897/aca.5.e87188 .

Popović, Slađana, Nikolić, Nataša, Pećić, Marija, Anđelković, Ana, Subakov SImić, Gordana, "Comparative analysis of lampenflora in two show caves in Serbia" in ARPHA Conference Abstracts (2022),
https://doi.org/10.3897/aca.5.e87188 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/684
AB  - Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected field (58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present in all samples, with Proteobacteria being the most prevalent, especially in tubers without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were more represented in tubers without symptoms. However, the genus Dickeya was at the limit of detection or not detected at all. Acinetobacter was the most dominant in tubers with soft rot, while Pseudomonas and Enterobacter were the most abundant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with starch-degradable features were almost exclusively present in soft rotted tubers and their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confirmed biocontrol potential. This study indicates that the soft rot maceration process arises due to complex interactions between plant pathogens and other endophytic bacteria.
PB  - Springer Verlag
T2  - Potato Research
T1  - Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres
DO  - 10.1007/s11540-022-09601-9
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Berić, Tanja and Dimkić, Ivica and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2022",
abstract = "Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected field (58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present in all samples, with Proteobacteria being the most prevalent, especially in tubers without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were more represented in tubers without symptoms. However, the genus Dickeya was at the limit of detection or not detected at all. Acinetobacter was the most dominant in tubers with soft rot, while Pseudomonas and Enterobacter were the most abundant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with starch-degradable features were almost exclusively present in soft rotted tubers and their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confirmed biocontrol potential. This study indicates that the soft rot maceration process arises due to complex interactions between plant pathogens and other endophytic bacteria.",
publisher = "Springer Verlag",
journal = "Potato Research",
title = "Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres",
doi = "10.1007/s11540-022-09601-9"
}

Marković, S., Popović Milovanović, T., Berić, T., Dimkić, I., Jelušić, A., Iličić, R.,& Stanković, S.. (2022). Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres. in Potato Research
Springer Verlag..
https://doi.org/10.1007/s11540-022-09601-9

Marković S, Popović Milovanović T, Berić T, Dimkić I, Jelušić A, Iličić R, Stanković S. Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres. in Potato Research. 2022;.
doi:10.1007/s11540-022-09601-9 .

Marković, Sanja, Popović Milovanović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres" in Potato Research (2022),
https://doi.org/10.1007/s11540-022-09601-9 . .

TY  - JOUR
AU  - Anđelković, Ana
AU  - Popović, Slađana
AU  - Živković, Milica
AU  - Cvijanović, Dušanka
AU  - Novković, Maja
AU  - Marisavljević, Dragana
AU  - Pavlovic, Danijela
AU  - Radulović, Snežana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/618
AB  - Portulaca oleracea L. is one of the most widely distributed plant species, invading a host of worldwide regions. Rivers and canals, as
corridors connecting neighboring habitats, are known to exhibit high invasion levels. Consequently, the aim of this paper was to show
which catchment areas, environmental factors and habitat types can be seen as predictors of the presence of this invasive species in the
riparian areas of Serbia. Field research was carried out at 250 field sites, where the cover and abundance of P. oleracea, relevant
environmental variables and habitat type were recorded. Redundancy analysis (RDA) and response curves fitted using a generalized
linear model (GLM) were used to show the relation of the cover of P. oleracea and the following variables: catchment area, predominant
bank material, elevation, and the total number of invasive species on site. A non-metric multidimensional scaling (NMDS) was used to
group sites where P. oleracea was found, based on the values of its cover. In general, primarily the rivers in the northern, low-lying part of
Serbia can be seen as important corridors of the spread of P. oleracea. Its dominance is especially associated with areas draining into the
Danube and Sava rivers and field sites characterized by a high pressure of invasive plants. Of the total number of records, the highest
number of invaded field sites was recorded along the Danube, Tisa and Zapadna Morava rivers. Regarding the environmental and habitat
predictors, the results have shown that the abundance of P. oleracea is associated with gravelly and sandy banks and grassland habitat
types, primarily anthropogenic herb stands.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta Agriculturae Serbica
T1  - Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia
EP  - 15
IS  - 53
SP  - 9
VL  - 27
DO  - 10.5937/AASer2253009A
ER  - 

@article{
author = "Anđelković, Ana and Popović, Slađana and Živković, Milica and Cvijanović, Dušanka and Novković, Maja and Marisavljević, Dragana and Pavlovic, Danijela and Radulović, Snežana",
year = "2022",
abstract = "Portulaca oleracea L. is one of the most widely distributed plant species, invading a host of worldwide regions. Rivers and canals, as
corridors connecting neighboring habitats, are known to exhibit high invasion levels. Consequently, the aim of this paper was to show
which catchment areas, environmental factors and habitat types can be seen as predictors of the presence of this invasive species in the
riparian areas of Serbia. Field research was carried out at 250 field sites, where the cover and abundance of P. oleracea, relevant
environmental variables and habitat type were recorded. Redundancy analysis (RDA) and response curves fitted using a generalized
linear model (GLM) were used to show the relation of the cover of P. oleracea and the following variables: catchment area, predominant
bank material, elevation, and the total number of invasive species on site. A non-metric multidimensional scaling (NMDS) was used to
group sites where P. oleracea was found, based on the values of its cover. In general, primarily the rivers in the northern, low-lying part of
Serbia can be seen as important corridors of the spread of P. oleracea. Its dominance is especially associated with areas draining into the
Danube and Sava rivers and field sites characterized by a high pressure of invasive plants. Of the total number of records, the highest
number of invaded field sites was recorded along the Danube, Tisa and Zapadna Morava rivers. Regarding the environmental and habitat
predictors, the results have shown that the abundance of P. oleracea is associated with gravelly and sandy banks and grassland habitat
types, primarily anthropogenic herb stands.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta Agriculturae Serbica",
title = "Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia",
pages = "15-9",
number = "53",
volume = "27",
doi = "10.5937/AASer2253009A"
}

Anđelković, A., Popović, S., Živković, M., Cvijanović, D., Novković, M., Marisavljević, D., Pavlovic, D.,& Radulović, S.. (2022). Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia. in Acta Agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 27(53), 9-15.
https://doi.org/10.5937/AASer2253009A

Anđelković A, Popović S, Živković M, Cvijanović D, Novković M, Marisavljević D, Pavlovic D, Radulović S. Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia. in Acta Agriculturae Serbica. 2022;27(53):9-15.
doi:10.5937/AASer2253009A .

Anđelković, Ana, Popović, Slađana, Živković, Milica, Cvijanović, Dušanka, Novković, Maja, Marisavljević, Dragana, Pavlovic, Danijela, Radulović, Snežana, "Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia" in Acta Agriculturae Serbica, 27, no. 53 (2022):9-15,
https://doi.org/10.5937/AASer2253009A . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Stanisavljević, Rade
AU  - Dimkić, Ivica
AU  - Velijević, Nataša
AU  - Soković, Marina
AU  - Ćirić, Ana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/700
AB  - Lolium multiflorum;early dormancy;desiccation;biocontrol agent;biostimulant;fungi inhibitory activity
Potential of Trichoderma harzianum IS005-12 (TH-IS005-12) to promote seed germination and seedling growth of Italian ryegrass (IRG) forage was evaluated in vitro. Non-desiccated seeds and those pre-harvestly desiccated with total herbicide were treated 25 days (freshly harvested) and 178 days after harvest (mature) with TH-IS005-12 spore suspensions at 0 (T0), 1.8 × 107 (T1) and 1.2 × 109 (T2) spore/mL. TH-IS005-12 promoted the early and final germination and seedling growth in all non-desiccated and desiccated, freshly harvested as well as mature IRG seeds. It was more effective in pre-harvestly desiccated freshly harvested seeds where T2 treatment increased final germination rate for 24%, root number per seedling 1.6-fold and seedling vigour 1.9-fold compared to the untreated control. Moreover, TH-IS005-12 showed an inhibitory activity against seedborne fungi Alternaria alternata and A. ventricosa suppressing their growth in vitro by 82% and 77%, respectively.
PB  - Czech Academy of Agricultural Sciences
T2  - Plant, Soil and Environment
T1  - Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage
EP  - 136
SP  - 130
VL  - 67
DO  - 10.17221/581/2020-PSE
ER  - 

@article{
author = "Banjac, Nevena and Stanisavljević, Rade and Dimkić, Ivica and Velijević, Nataša and Soković, Marina and Ćirić, Ana",
year = "2021",
abstract = "Lolium multiflorum;early dormancy;desiccation;biocontrol agent;biostimulant;fungi inhibitory activity
Potential of Trichoderma harzianum IS005-12 (TH-IS005-12) to promote seed germination and seedling growth of Italian ryegrass (IRG) forage was evaluated in vitro. Non-desiccated seeds and those pre-harvestly desiccated with total herbicide were treated 25 days (freshly harvested) and 178 days after harvest (mature) with TH-IS005-12 spore suspensions at 0 (T0), 1.8 × 107 (T1) and 1.2 × 109 (T2) spore/mL. TH-IS005-12 promoted the early and final germination and seedling growth in all non-desiccated and desiccated, freshly harvested as well as mature IRG seeds. It was more effective in pre-harvestly desiccated freshly harvested seeds where T2 treatment increased final germination rate for 24%, root number per seedling 1.6-fold and seedling vigour 1.9-fold compared to the untreated control. Moreover, TH-IS005-12 showed an inhibitory activity against seedborne fungi Alternaria alternata and A. ventricosa suppressing their growth in vitro by 82% and 77%, respectively.",
publisher = "Czech Academy of Agricultural Sciences",
journal = "Plant, Soil and Environment",
title = "Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage",
pages = "136-130",
volume = "67",
doi = "10.17221/581/2020-PSE"
}

Banjac, N., Stanisavljević, R., Dimkić, I., Velijević, N., Soković, M.,& Ćirić, A.. (2021). Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage. in Plant, Soil and Environment
Czech Academy of Agricultural Sciences., 67, 130-136.
https://doi.org/10.17221/581/2020-PSE

Banjac N, Stanisavljević R, Dimkić I, Velijević N, Soković M, Ćirić A. Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage. in Plant, Soil and Environment. 2021;67:130-136.
doi:10.17221/581/2020-PSE .

Banjac, Nevena, Stanisavljević, Rade, Dimkić, Ivica, Velijević, Nataša, Soković, Marina, Ćirić, Ana, "Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage" in Plant, Soil and Environment, 67 (2021):130-136,
https://doi.org/10.17221/581/2020-PSE . .

TY  - JOUR
AU  - Popović, Slađana
AU  - Nikolić, Nataša
AU  - Predojević, Dragana
AU  - Jakovljevic, Olga
AU  - Rađa, Tonći
AU  - Anđelković, Ana
AU  - Subakov Simic, Gordana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/837
AB  - Endoliths are more frequently found in extremely harsh environments, but they can inhabit mesic climate and subterranean habitats as well. Whether they are adapted to life inside rocks or do they seek refuge in this way, remains largely unknown for caves and pits. Consequently, subterranean habitats in limestone areas, were explored: three caves in Serbia (Cerjanska, Petnička and Potpeć) and one pit in Croatia (Keranova Golubinka). Sampling of rock substratum containing endoliths was performed at various distances from the entrance, at sites characterized by a lower level of light intensity. Phototrophs were separated from the rock matrix using 10% hydrochloric acid and analyzed using light microscopy. In general, low diversity of endoliths was observed, but representatives from Cyanobacteria, Chlorophyta and Xanthophyta were found. Cyanobacteria, more precisely coccoid forms (genera Aphanocapsa, Chroococcidiopsis, Gloeocapsa, Gloeocapsopsis, Synechococcus), were dominant. The most interesting cyanobacterial taxon, Leptolyngbya-like taxon, representing a true endolith (euendolith), was found in the Petnička Cave. This taxon and its relation to the substratum, as well as chasmoendolithic community from the Keranova Golubinka Pit, were observed using Scanning electron microscopy (SEM). It was observed that chasmoendoliths inhabit already existing surface rock cracks and fissures, while a true endolith actively bores into the substratum. Non-metric multidimensional scaling (NMDS) was performed to observe the similarity between the sampling sites. Endoliths can grow inside the rocks, making them very successful biodeteriogens, which is not a desirable trait for caves and pits that are characterized by various attractive geomorphological features that need to be preserved. Thus, considering these habitats, more attention should be paid to the endolith community in the future.
PB  - Kragujevac : University of Kragujevac - Faculty of Science
T2  - Kragujevac Journal of Science
T1  - Endolithic phototrophs: Examples from cave-like environments
EP  - 137
SP  - 123
VL  - 43
DO  - 10.5937/KgJSci2143123P
ER  - 

@article{
author = "Popović, Slađana and Nikolić, Nataša and Predojević, Dragana and Jakovljevic, Olga and Rađa, Tonći and Anđelković, Ana and Subakov Simic, Gordana",
year = "2021",
abstract = "Endoliths are more frequently found in extremely harsh environments, but they can inhabit mesic climate and subterranean habitats as well. Whether they are adapted to life inside rocks or do they seek refuge in this way, remains largely unknown for caves and pits. Consequently, subterranean habitats in limestone areas, were explored: three caves in Serbia (Cerjanska, Petnička and Potpeć) and one pit in Croatia (Keranova Golubinka). Sampling of rock substratum containing endoliths was performed at various distances from the entrance, at sites characterized by a lower level of light intensity. Phototrophs were separated from the rock matrix using 10% hydrochloric acid and analyzed using light microscopy. In general, low diversity of endoliths was observed, but representatives from Cyanobacteria, Chlorophyta and Xanthophyta were found. Cyanobacteria, more precisely coccoid forms (genera Aphanocapsa, Chroococcidiopsis, Gloeocapsa, Gloeocapsopsis, Synechococcus), were dominant. The most interesting cyanobacterial taxon, Leptolyngbya-like taxon, representing a true endolith (euendolith), was found in the Petnička Cave. This taxon and its relation to the substratum, as well as chasmoendolithic community from the Keranova Golubinka Pit, were observed using Scanning electron microscopy (SEM). It was observed that chasmoendoliths inhabit already existing surface rock cracks and fissures, while a true endolith actively bores into the substratum. Non-metric multidimensional scaling (NMDS) was performed to observe the similarity between the sampling sites. Endoliths can grow inside the rocks, making them very successful biodeteriogens, which is not a desirable trait for caves and pits that are characterized by various attractive geomorphological features that need to be preserved. Thus, considering these habitats, more attention should be paid to the endolith community in the future.",
publisher = "Kragujevac : University of Kragujevac - Faculty of Science",
journal = "Kragujevac Journal of Science",
title = "Endolithic phototrophs: Examples from cave-like environments",
pages = "137-123",
volume = "43",
doi = "10.5937/KgJSci2143123P"
}

Popović, S., Nikolić, N., Predojević, D., Jakovljevic, O., Rađa, T., Anđelković, A.,& Subakov Simic, G.. (2021). Endolithic phototrophs: Examples from cave-like environments. in Kragujevac Journal of Science
Kragujevac : University of Kragujevac - Faculty of Science., 43, 123-137.
https://doi.org/10.5937/KgJSci2143123P

Popović S, Nikolić N, Predojević D, Jakovljevic O, Rađa T, Anđelković A, Subakov Simic G. Endolithic phototrophs: Examples from cave-like environments. in Kragujevac Journal of Science. 2021;43:123-137.
doi:10.5937/KgJSci2143123P .

Popović, Slađana, Nikolić, Nataša, Predojević, Dragana, Jakovljevic, Olga, Rađa, Tonći, Anđelković, Ana, Subakov Simic, Gordana, "Endolithic phototrophs: Examples from cave-like environments" in Kragujevac Journal of Science, 43 (2021):123-137,
https://doi.org/10.5937/KgJSci2143123P . .

TY  - CONF
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/798
AB  - Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi distribucija populacija u 2020. i 2021. godini. Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze (PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano (MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021
T1  - NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI
ER  - 

@conference{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi distribucija populacija u 2020. i 2021. godini. Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze (PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano (MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021",
title = "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI"
}

Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović Milovanović, T.. (2021). NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021
Društvo za zaštitu bilja Srbije..

Marković S, Stanković S, Jelušić A, Iličić R, Popović Milovanović T. NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021. 2021;..

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović Milovanović, Tatjana, "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021 (2021).

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peters, Kelly
AU  - Miklavcic-Visnjevec, Ana
AU  - Tavzes, Črtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/645
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - •	Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peters, Kelly and Miklavcic-Visnjevec, Ana and Tavzes, Črtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "•	Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović Milovanović, T., Dimkić, I., Mitrović, P., Peters, K., Miklavcic-Visnjevec, A., Tavzes, Č., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
•	Academic Press Inc Elsevier Science, San Diego., 160.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović Milovanović T, Dimkić I, Mitrović P, Peters K, Miklavcic-Visnjevec A, Tavzes Č, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peters, Kelly, Miklavcic-Visnjevec, Ana, Tavzes, Črtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021),
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Iličić, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/740
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013–2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2–22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3–5 were highly intensified in response to R. solanacearum.
PB  - British Society for Plant Pathology
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
DO  - 0032-0862
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Iličić, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013–2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2–22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3–5 were highly intensified in response to R. solanacearum.",
publisher = "British Society for Plant Pathology",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421, 0032-0862"
}

Marković, S., Stanković, S., Iličić, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović Milovanović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
British Society for Plant Pathology., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Iličić R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović Milovanović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Iličić, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović Milovanović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .