TY  - JOUR
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/690
AB  - Diseases associated with ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) are of great economic importance, causing severe damage on a wide range of crops. Polyphagous insect vectors and wild plants acting as natural reservoir both play a major role in the epidemiology of phytoplasmas directly determining their transmission and persistence in agroecosystems. This is especially the case for stolbur phytoplasma diseases induced by ‘Ca. P. solani’ a pathogen native to Europe and with a plethora of natural reservoir plants and insect vectors, some being host specific and some being generalists. Fluctuating market demands shape farming in Serbia, often limiting production to narrow or repetitive crop rotation, providing favorable conditions for continuous re-appearance of stolbur phytoplasma epidemics. Our aim was to generate scenarios in which relevant biological factors (e.g. susceptibility of crops, natural reservoirs of phytoplasma, insect vectors and their host preferences), may threat crop fields by triggering re-emergence of stolbur epidemics. Our specific objective was to assess the potential risks and to define the best management practices to cope with disease outbreaks. The first step was building an easy-to-follow online free Compendium for farmers with the list of: a) all validated host plants for stolbur phytoplasma, including both cultivated and wild plants and, b) all known insect vectors and their hosts. The Compendium also serves as a platform for a risk assessment framework integrated with a decision-making scheme that proposes different options for land use architecture and crop allocation at diverse temporal and spatial scales.
PB  - Springer Verlag
T2  - Journal of Plant Pathology
T1  - Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia
EP  - 552
SP  - 537
VL  - 104
DO  - 10.1007/s42161-022-01055-9
ER  - 

@article{
author = "Mitrović, Milana and Marinković, Slavica and Cvrković, Tatjana and Jovic, Jelena and Krstić, Oliver and Jakovljević, Miljana",
year = "2022",
abstract = "Diseases associated with ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) are of great economic importance, causing severe damage on a wide range of crops. Polyphagous insect vectors and wild plants acting as natural reservoir both play a major role in the epidemiology of phytoplasmas directly determining their transmission and persistence in agroecosystems. This is especially the case for stolbur phytoplasma diseases induced by ‘Ca. P. solani’ a pathogen native to Europe and with a plethora of natural reservoir plants and insect vectors, some being host specific and some being generalists. Fluctuating market demands shape farming in Serbia, often limiting production to narrow or repetitive crop rotation, providing favorable conditions for continuous re-appearance of stolbur phytoplasma epidemics. Our aim was to generate scenarios in which relevant biological factors (e.g. susceptibility of crops, natural reservoirs of phytoplasma, insect vectors and their host preferences), may threat crop fields by triggering re-emergence of stolbur epidemics. Our specific objective was to assess the potential risks and to define the best management practices to cope with disease outbreaks. The first step was building an easy-to-follow online free Compendium for farmers with the list of: a) all validated host plants for stolbur phytoplasma, including both cultivated and wild plants and, b) all known insect vectors and their hosts. The Compendium also serves as a platform for a risk assessment framework integrated with a decision-making scheme that proposes different options for land use architecture and crop allocation at diverse temporal and spatial scales.",
publisher = "Springer Verlag",
journal = "Journal of Plant Pathology",
title = "Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia",
pages = "552-537",
volume = "104",
doi = "10.1007/s42161-022-01055-9"
}

Mitrović, M., Marinković, S., Cvrković, T., Jovic, J., Krstić, O.,& Jakovljević, M.. (2022). Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia. in Journal of Plant Pathology
Springer Verlag., 104, 537-552.
https://doi.org/10.1007/s42161-022-01055-9

Mitrović M, Marinković S, Cvrković T, Jovic J, Krstić O, Jakovljević M. Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia. in Journal of Plant Pathology. 2022;104:537-552.
doi:10.1007/s42161-022-01055-9 .

Mitrović, Milana, Marinković, Slavica, Cvrković, Tatjana, Jovic, Jelena, Krstić, Oliver, Jakovljević, Miljana, "Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia" in Journal of Plant Pathology, 104 (2022):537-552,
https://doi.org/10.1007/s42161-022-01055-9 . .

TY  - JOUR
AU  - Jovic, Jelena
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/693
AB  - Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs
of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse
agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely
related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that
is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms
association of the two phytoplasmas in shared host plants were the subject of this study. Specific
primers for the amplification of the elongation factor Tu gene (tuf ) were developed for the identification
of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous
detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among
symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca.
P. solani’ and ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas.
None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants
from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected
solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in
plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35)
were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’.
Variations in symptoms and their association with each of the phytoplasmas are described and
documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’
infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific
alterations in infected bindweeds. The results are discussed in the context of the epidemiological
and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two
phytoplasma relatives in shared host plant.
PB  - MDPI Basel
T2  - Pathogens
T1  - Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed
IS  - 2
SP  - 160
VL  - 10
DO  - 10.3390/pathogens10020160
ER  - 

@article{
author = "Jovic, Jelena and Marinković, Slavica and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana",
year = "2021",
abstract = "Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs
of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse
agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely
related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that
is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms
association of the two phytoplasmas in shared host plants were the subject of this study. Specific
primers for the amplification of the elongation factor Tu gene (tuf ) were developed for the identification
of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous
detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among
symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca.
P. solani’ and ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas.
None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants
from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected
solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in
plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35)
were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’.
Variations in symptoms and their association with each of the phytoplasmas are described and
documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’
infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific
alterations in infected bindweeds. The results are discussed in the context of the epidemiological
and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two
phytoplasma relatives in shared host plant.",
publisher = "MDPI Basel",
journal = "Pathogens",
title = "Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed",
number = "2",
pages = "160",
volume = "10",
doi = "10.3390/pathogens10020160"
}

Jovic, J., Marinković, S., Jakovljević, M., Krstić, O., Cvrković, T.,& Mitrović, M.. (2021). Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed. in Pathogens
MDPI Basel., 10(2), 160.
https://doi.org/10.3390/pathogens10020160

Jovic J, Marinković S, Jakovljević M, Krstić O, Cvrković T, Mitrović M. Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed. in Pathogens. 2021;10(2):160.
doi:10.3390/pathogens10020160 .

Jovic, Jelena, Marinković, Slavica, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, "Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed" in Pathogens, 10, no. 2 (2021):160,
https://doi.org/10.3390/pathogens10020160 . .