TY  - JOUR
AU  - Đalović, Ivica
AU  - Mitrović, Petar
AU  - Trivan, Goran
AU  - Jelušić, Aleksandra
AU  - Pezo, Lato
AU  - Janić Hajnal, Elizabet
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1246
AB  - Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics
of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to
significant economic losses. The aim of this study was to determine biochemical changes in peony
(Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca.
Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural
network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains
originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill
strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical
parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins
contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions
and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation
between different chemical parameters and revealed a clear separation among the samples. Based on
the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP,
NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced
high r2 values (1.000 during the training period) and low SOS values. Developing an effective early
warning system for the detection of plant diseases in different plant species is critical for improving
crop yield and quality.
PB  - MDPI Basel
T2  - Horticulturae
T1  - The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach
IS  - 5
SP  - 426
VL  - 10
DO  - 10.3390/horticulturae10050426
ER  - 

@article{
author = "Đalović, Ivica and Mitrović, Petar and Trivan, Goran and Jelušić, Aleksandra and Pezo, Lato and Janić Hajnal, Elizabet and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics
of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to
significant economic losses. The aim of this study was to determine biochemical changes in peony
(Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca.
Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural
network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains
originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill
strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical
parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins
contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions
and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation
between different chemical parameters and revealed a clear separation among the samples. Based on
the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP,
NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced
high r2 values (1.000 during the training period) and low SOS values. Developing an effective early
warning system for the detection of plant diseases in different plant species is critical for improving
crop yield and quality.",
publisher = "MDPI Basel",
journal = "Horticulturae",
title = "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach",
number = "5",
pages = "426",
volume = "10",
doi = "10.3390/horticulturae10050426"
}

Đalović, I., Mitrović, P., Trivan, G., Jelušić, A., Pezo, L., Janić Hajnal, E.,& Popović Milovanović, T.. (2024). The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae
MDPI Basel., 10(5), 426.
https://doi.org/10.3390/horticulturae10050426

Đalović I, Mitrović P, Trivan G, Jelušić A, Pezo L, Janić Hajnal E, Popović Milovanović T. The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae. 2024;10(5):426.
doi:10.3390/horticulturae10050426 .

Đalović, Ivica, Mitrović, Petar, Trivan, Goran, Jelušić, Aleksandra, Pezo, Lato, Janić Hajnal, Elizabet, Popović Milovanović, Tatjana, "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach" in Horticulturae, 10, no. 5 (2024):426,
https://doi.org/10.3390/horticulturae10050426 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1137
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1138
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
IS  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8, 2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8).
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8).
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023),
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Anđelković, Ana
AU  - Pavlovic, Danijela
AU  - Marisavljević, Dragana
AU  - Živković, Milica
AU  - Novković, Maja
AU  - Popović, Slađana
AU  - Cvijanović, Dušanka
AU  - Radulović, Snežana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/728
AB  - Riparian areas experience strong invasion pressures worldwide and represent important points of spread for invasive alien plants (IAPs) in the European mainland. The Danube Basin is a well-known point of high plant invasion levels. Given that the middle part of the Danube Basin is critically understudied and the general lack of data for Serbia, the study aimed to provide an insight into the spatial patterns of plant invasions in the riparian areas of Serbia (Middle Danube Basin area). A total of 250 field sites, distributed along 39 rivers (nine catchment areas) and six canal sections, were studied during a four-year period (2013–2016) for the presence and abundance of IAPs. At the landscape scale, we studied distribution patterns of IAPs, differences in invasion levels in different catchment areas and between rivers and canals. At the local scale, we investigated how the proximity to roads/railway lines, housing areas, different land-use types (primarily agriculture), and dominant vegetation on site related to invasion patterns. Of the 26 studied IAPs, those with a well-known weedy behavior, long history of cultivation and strong affinity for riparian areas prevailed in the study area. Riparian zones of the Danube catchment exhibited the highest invasion levels in terms of IAPs richness and abundance, followed by the catchment areas of the Timok, Sava and Zapadna Morava rivers. Surprisingly, the Danube-Tisa-Danube canal network had the lowest invasion level. At the local scale, agriculture in proximity of the field site and dominant vegetation on site were observed as significant predictors of the invasion level. On the other hand, proximity to roads/railway lines and housing areas was not related to the invasion level. Finally, our study provides the first systematic overview of IAPs’ distribution data for riparian areas of the Middle Danube Basin in Serbia, which could provide a basis for long-term monitoring of IAPs and development of future management plans.
PB  - Pensoft Publishers
T2  - NeoBiota
T1  - Plant invasions in riparian areas of the Middle Danube Basin in Serbia
IS  - 23
VL  - 71
DO  - 10.3897/neobiota.71.69716
ER  - 

@article{
author = "Anđelković, Ana and Pavlovic, Danijela and Marisavljević, Dragana and Živković, Milica and Novković, Maja and Popović, Slađana and Cvijanović, Dušanka and Radulović, Snežana",
year = "2022",
abstract = "Riparian areas experience strong invasion pressures worldwide and represent important points of spread for invasive alien plants (IAPs) in the European mainland. The Danube Basin is a well-known point of high plant invasion levels. Given that the middle part of the Danube Basin is critically understudied and the general lack of data for Serbia, the study aimed to provide an insight into the spatial patterns of plant invasions in the riparian areas of Serbia (Middle Danube Basin area). A total of 250 field sites, distributed along 39 rivers (nine catchment areas) and six canal sections, were studied during a four-year period (2013–2016) for the presence and abundance of IAPs. At the landscape scale, we studied distribution patterns of IAPs, differences in invasion levels in different catchment areas and between rivers and canals. At the local scale, we investigated how the proximity to roads/railway lines, housing areas, different land-use types (primarily agriculture), and dominant vegetation on site related to invasion patterns. Of the 26 studied IAPs, those with a well-known weedy behavior, long history of cultivation and strong affinity for riparian areas prevailed in the study area. Riparian zones of the Danube catchment exhibited the highest invasion levels in terms of IAPs richness and abundance, followed by the catchment areas of the Timok, Sava and Zapadna Morava rivers. Surprisingly, the Danube-Tisa-Danube canal network had the lowest invasion level. At the local scale, agriculture in proximity of the field site and dominant vegetation on site were observed as significant predictors of the invasion level. On the other hand, proximity to roads/railway lines and housing areas was not related to the invasion level. Finally, our study provides the first systematic overview of IAPs’ distribution data for riparian areas of the Middle Danube Basin in Serbia, which could provide a basis for long-term monitoring of IAPs and development of future management plans.",
publisher = "Pensoft Publishers",
journal = "NeoBiota",
title = "Plant invasions in riparian areas of the Middle Danube Basin in Serbia",
number = "23",
volume = "71",
doi = "10.3897/neobiota.71.69716"
}

Anđelković, A., Pavlovic, D., Marisavljević, D., Živković, M., Novković, M., Popović, S., Cvijanović, D.,& Radulović, S.. (2022). Plant invasions in riparian areas of the Middle Danube Basin in Serbia. in NeoBiota
Pensoft Publishers., 71(23).
https://doi.org/10.3897/neobiota.71.69716

Anđelković A, Pavlovic D, Marisavljević D, Živković M, Novković M, Popović S, Cvijanović D, Radulović S. Plant invasions in riparian areas of the Middle Danube Basin in Serbia. in NeoBiota. 2022;71(23).
doi:10.3897/neobiota.71.69716 .

Anđelković, Ana, Pavlovic, Danijela, Marisavljević, Dragana, Živković, Milica, Novković, Maja, Popović, Slađana, Cvijanović, Dušanka, Radulović, Snežana, "Plant invasions in riparian areas of the Middle Danube Basin in Serbia" in NeoBiota, 71, no. 23 (2022),
https://doi.org/10.3897/neobiota.71.69716 . .

TY  - JOUR
AU  - Šaulić, Marko
AU  - Oveisi, Mostafa
AU  - Đalović, Ivica
AU  - Božić, Dragana
AU  - Pishyar, Alireza
AU  - Savić, Aleksandra
AU  - Prasad, Vara
AU  - Vrbničanin, Sava
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/733
AB  - Crop rotation is known as an eco-friendlier approach, as provides diversification in crop management systems, modifies intensive pressure on the agricultural ecosystem, utilizes various soil horizons, and prevents the establishment of specific pests and weeds. We set out here a study on the farms that have been managed over 50 years of specific continuous crop management programs. The experimental treatments were in a different management system: monoculture of maize, winter wheat, and soybean, 2-year crop rotation (winter wheat–maize) with and without chemical fertilizer, and 3-year crop rotation (winter wheat–soybean–maize) with and without chemical fertilizer and manure. We took soil samples six times from 2014 to 2017 prior to sowing and after harvesting each year. Weed seeds were extracted from soils and identified and counted by species. We, upon the data of a long term experiment of crop rotation, could conclude that crop rotations with more crops in the sequence are significantly effective in maintaining weed populations, a low-input crop production could reach a constant low population below an important damaging density, and therefore will be a more sustainable crop production while chemical fertilizers would change the soil’s chemical and structure and imbalance the plant population diversity and manures with high weed seed infestations have the potential to totally eradicate crop rotation effects, Therefore, clean manures or compost are highly recommended.
PB  - MDPI
T2  - Agronomy-Basel
T1  - How Do Long Term Crop Rotations Influence Weed Populations: Exploring the Impacts of More than 50 Years of Crop Management in Serbia
IS  - 8
SP  - 1772
VL  - 12
DO  - 10.3390/agronomy12081772
ER  - 

@article{
author = "Šaulić, Marko and Oveisi, Mostafa and Đalović, Ivica and Božić, Dragana and Pishyar, Alireza and Savić, Aleksandra and Prasad, Vara and Vrbničanin, Sava",
year = "2022",
abstract = "Crop rotation is known as an eco-friendlier approach, as provides diversification in crop management systems, modifies intensive pressure on the agricultural ecosystem, utilizes various soil horizons, and prevents the establishment of specific pests and weeds. We set out here a study on the farms that have been managed over 50 years of specific continuous crop management programs. The experimental treatments were in a different management system: monoculture of maize, winter wheat, and soybean, 2-year crop rotation (winter wheat–maize) with and without chemical fertilizer, and 3-year crop rotation (winter wheat–soybean–maize) with and without chemical fertilizer and manure. We took soil samples six times from 2014 to 2017 prior to sowing and after harvesting each year. Weed seeds were extracted from soils and identified and counted by species. We, upon the data of a long term experiment of crop rotation, could conclude that crop rotations with more crops in the sequence are significantly effective in maintaining weed populations, a low-input crop production could reach a constant low population below an important damaging density, and therefore will be a more sustainable crop production while chemical fertilizers would change the soil’s chemical and structure and imbalance the plant population diversity and manures with high weed seed infestations have the potential to totally eradicate crop rotation effects, Therefore, clean manures or compost are highly recommended.",
publisher = "MDPI",
journal = "Agronomy-Basel",
title = "How Do Long Term Crop Rotations Influence Weed Populations: Exploring the Impacts of More than 50 Years of Crop Management in Serbia",
number = "8",
pages = "1772",
volume = "12",
doi = "10.3390/agronomy12081772"
}

Šaulić, M., Oveisi, M., Đalović, I., Božić, D., Pishyar, A., Savić, A., Prasad, V.,& Vrbničanin, S.. (2022). How Do Long Term Crop Rotations Influence Weed Populations: Exploring the Impacts of More than 50 Years of Crop Management in Serbia. in Agronomy-Basel
MDPI., 12(8), 1772.
https://doi.org/10.3390/agronomy12081772

Šaulić M, Oveisi M, Đalović I, Božić D, Pishyar A, Savić A, Prasad V, Vrbničanin S. How Do Long Term Crop Rotations Influence Weed Populations: Exploring the Impacts of More than 50 Years of Crop Management in Serbia. in Agronomy-Basel. 2022;12(8):1772.
doi:10.3390/agronomy12081772 .

Šaulić, Marko, Oveisi, Mostafa, Đalović, Ivica, Božić, Dragana, Pishyar, Alireza, Savić, Aleksandra, Prasad, Vara, Vrbničanin, Sava, "How Do Long Term Crop Rotations Influence Weed Populations: Exploring the Impacts of More than 50 Years of Crop Management in Serbia" in Agronomy-Basel, 12, no. 8 (2022):1772,
https://doi.org/10.3390/agronomy12081772 . .

TY  - JOUR
AU  - Pavlovic, Danijela
AU  - Vrbničanin, Sava
AU  - Anđelković, Ana
AU  - Božić, Dragana
AU  - Rajković, Miloš
AU  - Malidža, Goran
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/731
AB  - Herbicide application has long been considered the most efficient weed control method
in agricultural production worldwide. However, long-term use of agrochemicals has numerous
negative effects on crops and the environment. Bearing in mind these negative impacts, the EU
strategy for withdrawing many herbicides from use, and modern market demands for the production
of healthy and safe food, there is a need for developing new effective, sustainable, and ecological
weed control measures. To bring a fresh perspective on this topic, this paper aims to describe the most
important non-chemical weed control strategies, including ecological integrated weed management
(EIWM), limiting weed seed bank, site-specific weed management, mechanical weeding, mulching,
crop competitiveness, intercropping, subsidiary crops, green manure, and bioherbicides.
PB  - MDPI
T2  - Agronomy
T1  - Non-Chemical Weed Control for Plant Health and Environment: Ecological Integrated Weed Management (EIWM)
SP  - 1091
VL  - 12
VL  - 5
DO  - 10.3390/agronomy12051091
ER  - 

@article{
author = "Pavlovic, Danijela and Vrbničanin, Sava and Anđelković, Ana and Božić, Dragana and Rajković, Miloš and Malidža, Goran",
year = "2022",
abstract = "Herbicide application has long been considered the most efficient weed control method
in agricultural production worldwide. However, long-term use of agrochemicals has numerous
negative effects on crops and the environment. Bearing in mind these negative impacts, the EU
strategy for withdrawing many herbicides from use, and modern market demands for the production
of healthy and safe food, there is a need for developing new effective, sustainable, and ecological
weed control measures. To bring a fresh perspective on this topic, this paper aims to describe the most
important non-chemical weed control strategies, including ecological integrated weed management
(EIWM), limiting weed seed bank, site-specific weed management, mechanical weeding, mulching,
crop competitiveness, intercropping, subsidiary crops, green manure, and bioherbicides.",
publisher = "MDPI",
journal = "Agronomy",
title = "Non-Chemical Weed Control for Plant Health and Environment: Ecological Integrated Weed Management (EIWM)",
pages = "1091",
volume = "12, 5",
doi = "10.3390/agronomy12051091"
}

Pavlovic, D., Vrbničanin, S., Anđelković, A., Božić, D., Rajković, M.,& Malidža, G.. (2022). Non-Chemical Weed Control for Plant Health and Environment: Ecological Integrated Weed Management (EIWM). in Agronomy
MDPI., 12, 1091.
https://doi.org/10.3390/agronomy12051091

Pavlovic D, Vrbničanin S, Anđelković A, Božić D, Rajković M, Malidža G. Non-Chemical Weed Control for Plant Health and Environment: Ecological Integrated Weed Management (EIWM). in Agronomy. 2022;12:1091.
doi:10.3390/agronomy12051091 .

Pavlovic, Danijela, Vrbničanin, Sava, Anđelković, Ana, Božić, Dragana, Rajković, Miloš, Malidža, Goran, "Non-Chemical Weed Control for Plant Health and Environment: Ecological Integrated Weed Management (EIWM)" in Agronomy, 12 (2022):1091,
https://doi.org/10.3390/agronomy12051091 . .

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ivanović, Žarko
AU  - Ignjatov, Maja
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Grahovac, Mila
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/685
AB  - Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Genetic Diversity of Pectobacterium spp. on Potato in Serbia
IS  - 9
VL  - 10
DO  - 10.3390/microorganisms10091840
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ivanović, Žarko and Ignjatov, Maja and Budakov, Dragana and Grahovac, Jovana and Grahovac, Mila",
year = "2022",
abstract = "Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Genetic Diversity of Pectobacterium spp. on Potato in Serbia",
number = "9",
volume = "10",
doi = "10.3390/microorganisms10091840"
}

Loc, M., Milošević, D., Ivanović, Ž., Ignjatov, M., Budakov, D., Grahovac, J.,& Grahovac, M.. (2022). Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms
MDPI., 10(9).
https://doi.org/10.3390/microorganisms10091840

Loc M, Milošević D, Ivanović Ž, Ignjatov M, Budakov D, Grahovac J, Grahovac M. Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms. 2022;10(9).
doi:10.3390/microorganisms10091840 .

Loc, Marta, Milošević, Dragana, Ivanović, Žarko, Ignjatov, Maja, Budakov, Dragana, Grahovac, Jovana, Grahovac, Mila, "Genetic Diversity of Pectobacterium spp. on Potato in Serbia" in Microorganisms, 10, no. 9 (2022),
https://doi.org/10.3390/microorganisms10091840 . .

TY  - JOUR
AU  - Anđelković, Ana
AU  - Popović, Slađana
AU  - Živković, Milica
AU  - Cvijanović, Dušanka
AU  - Novković, Maja
AU  - Marisavljević, Dragana
AU  - Pavlovic, Danijela
AU  - Radulović, Snežana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/618
AB  - Portulaca oleracea L. is one of the most widely distributed plant species, invading a host of worldwide regions. Rivers and canals, as
corridors connecting neighboring habitats, are known to exhibit high invasion levels. Consequently, the aim of this paper was to show
which catchment areas, environmental factors and habitat types can be seen as predictors of the presence of this invasive species in the
riparian areas of Serbia. Field research was carried out at 250 field sites, where the cover and abundance of P. oleracea, relevant
environmental variables and habitat type were recorded. Redundancy analysis (RDA) and response curves fitted using a generalized
linear model (GLM) were used to show the relation of the cover of P. oleracea and the following variables: catchment area, predominant
bank material, elevation, and the total number of invasive species on site. A non-metric multidimensional scaling (NMDS) was used to
group sites where P. oleracea was found, based on the values of its cover. In general, primarily the rivers in the northern, low-lying part of
Serbia can be seen as important corridors of the spread of P. oleracea. Its dominance is especially associated with areas draining into the
Danube and Sava rivers and field sites characterized by a high pressure of invasive plants. Of the total number of records, the highest
number of invaded field sites was recorded along the Danube, Tisa and Zapadna Morava rivers. Regarding the environmental and habitat
predictors, the results have shown that the abundance of P. oleracea is associated with gravelly and sandy banks and grassland habitat
types, primarily anthropogenic herb stands.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta Agriculturae Serbica
T1  - Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia
EP  - 15
IS  - 53
SP  - 9
VL  - 27
DO  - 10.5937/AASer2253009A
ER  - 

@article{
author = "Anđelković, Ana and Popović, Slađana and Živković, Milica and Cvijanović, Dušanka and Novković, Maja and Marisavljević, Dragana and Pavlovic, Danijela and Radulović, Snežana",
year = "2022",
abstract = "Portulaca oleracea L. is one of the most widely distributed plant species, invading a host of worldwide regions. Rivers and canals, as
corridors connecting neighboring habitats, are known to exhibit high invasion levels. Consequently, the aim of this paper was to show
which catchment areas, environmental factors and habitat types can be seen as predictors of the presence of this invasive species in the
riparian areas of Serbia. Field research was carried out at 250 field sites, where the cover and abundance of P. oleracea, relevant
environmental variables and habitat type were recorded. Redundancy analysis (RDA) and response curves fitted using a generalized
linear model (GLM) were used to show the relation of the cover of P. oleracea and the following variables: catchment area, predominant
bank material, elevation, and the total number of invasive species on site. A non-metric multidimensional scaling (NMDS) was used to
group sites where P. oleracea was found, based on the values of its cover. In general, primarily the rivers in the northern, low-lying part of
Serbia can be seen as important corridors of the spread of P. oleracea. Its dominance is especially associated with areas draining into the
Danube and Sava rivers and field sites characterized by a high pressure of invasive plants. Of the total number of records, the highest
number of invaded field sites was recorded along the Danube, Tisa and Zapadna Morava rivers. Regarding the environmental and habitat
predictors, the results have shown that the abundance of P. oleracea is associated with gravelly and sandy banks and grassland habitat
types, primarily anthropogenic herb stands.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta Agriculturae Serbica",
title = "Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia",
pages = "15-9",
number = "53",
volume = "27",
doi = "10.5937/AASer2253009A"
}

Anđelković, A., Popović, S., Živković, M., Cvijanović, D., Novković, M., Marisavljević, D., Pavlovic, D.,& Radulović, S.. (2022). Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia. in Acta Agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 27(53), 9-15.
https://doi.org/10.5937/AASer2253009A

Anđelković A, Popović S, Živković M, Cvijanović D, Novković M, Marisavljević D, Pavlovic D, Radulović S. Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia. in Acta Agriculturae Serbica. 2022;27(53):9-15.
doi:10.5937/AASer2253009A .

Anđelković, Ana, Popović, Slađana, Živković, Milica, Cvijanović, Dušanka, Novković, Maja, Marisavljević, Dragana, Pavlovic, Danijela, Radulović, Snežana, "Catchment area, environmental variables and habitat type as predictors of the distribution and abundance of Portulaca oleracea L. in the riparian areas of Serbia" in Acta Agriculturae Serbica, 27, no. 53 (2022):9-15,
https://doi.org/10.5937/AASer2253009A . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/625
AB  - Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 wаs identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot
EP  - 27
SP  - 21
VL  - 140
DO  - 10.2298/ZMSPN2140021I
ER  - 

@article{
author = "Ignjatov, Maja and Milošević, Dragana and Tamindžić, Gordana and Ivanović, Žarko",
year = "2021",
abstract = "Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 wаs identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot",
pages = "27-21",
volume = "140",
doi = "10.2298/ZMSPN2140021I"
}

Ignjatov, M., Milošević, D., Tamindžić, G.,& Ivanović, Ž.. (2021). Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 21-27.
https://doi.org/10.2298/ZMSPN2140021I

Ignjatov M, Milošević D, Tamindžić G, Ivanović Ž. Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot. in Zbornik Matice srpske za prirodne nauke. 2021;140:21-27.
doi:10.2298/ZMSPN2140021I .

Ignjatov, Maja, Milošević, Dragana, Tamindžić, Gordana, Ivanović, Žarko, "Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot" in Zbornik Matice srpske za prirodne nauke, 140 (2021):21-27,
https://doi.org/10.2298/ZMSPN2140021I . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Kosovac, Andrea
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/829
AB  - ‘Candidatus Phytoplasma solani’, known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during august 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for ‘Ca. P. solani’ presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stol11 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmp1. Combining molecular tools PCR/RFLP and sequencing reviled two ‘Ca. P. solani’ multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the ‘Ca. P. solani’ diversity on celery, but supplemented with genotyping of the vmp1 gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of ‘Ca. P. solani’ affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.
PB  - Novi Sad : Institut za ratarstvo i povrtarstvo
T2  - Ratarstvo i povrtarstvo/Field and Vegetable Crops Research
T1  - Molecular characterization of'Candidatus Phytoplasma solani'in celery: Case study in Futog
EP  - 71
IS  - 2
SP  - 66
VL  - 58
DO  - 10.5937/ratpov58-33227
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Mitrović, Petar and Kosovac, Andrea",
year = "2021",
abstract = "‘Candidatus Phytoplasma solani’, known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during august 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for ‘Ca. P. solani’ presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stol11 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmp1. Combining molecular tools PCR/RFLP and sequencing reviled two ‘Ca. P. solani’ multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the ‘Ca. P. solani’ diversity on celery, but supplemented with genotyping of the vmp1 gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of ‘Ca. P. solani’ affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.",
publisher = "Novi Sad : Institut za ratarstvo i povrtarstvo",
journal = "Ratarstvo i povrtarstvo/Field and Vegetable Crops Research",
title = "Molecular characterization of'Candidatus Phytoplasma solani'in celery: Case study in Futog",
pages = "71-66",
number = "2",
volume = "58",
doi = "10.5937/ratpov58-33227"
}

Popović Milovanović, T., Mitrović, P.,& Kosovac, A.. (2021). Molecular characterization of'Candidatus Phytoplasma solani'in celery: Case study in Futog. in Ratarstvo i povrtarstvo/Field and Vegetable Crops Research
Novi Sad : Institut za ratarstvo i povrtarstvo., 58(2), 66-71.
https://doi.org/10.5937/ratpov58-33227

Popović Milovanović T, Mitrović P, Kosovac A. Molecular characterization of'Candidatus Phytoplasma solani'in celery: Case study in Futog. in Ratarstvo i povrtarstvo/Field and Vegetable Crops Research. 2021;58(2):66-71.
doi:10.5937/ratpov58-33227 .

Popović Milovanović, Tatjana, Mitrović, Petar, Kosovac, Andrea, "Molecular characterization of'Candidatus Phytoplasma solani'in celery: Case study in Futog" in Ratarstvo i povrtarstvo/Field and Vegetable Crops Research, 58, no. 2 (2021):66-71,
https://doi.org/10.5937/ratpov58-33227 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peters, Kelly
AU  - Miklavcic-Visnjevec, Ana
AU  - Tavzes, Črtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/645
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - •	Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peters, Kelly and Miklavcic-Visnjevec, Ana and Tavzes, Črtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC-MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "•	Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović Milovanović, T., Dimkić, I., Mitrović, P., Peters, K., Miklavcic-Visnjevec, A., Tavzes, Č., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
•	Academic Press Inc Elsevier Science, San Diego., 160.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović Milovanović T, Dimkić I, Mitrović P, Peters K, Miklavcic-Visnjevec A, Tavzes Č, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peters, Kelly, Miklavcic-Visnjevec, Ana, Tavzes, Črtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021),
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Mitrović, Petar
AU  - Đalović, Ivica
AU  - Kiprovski, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/605
AB  - The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani
IS  - 2
VL  - 10
DO  - 10.3390/plants10020337
ER  - 

@article{
author = "Mitrović, Petar and Đalović, Ivica and Kiprovski, Biljana and Veljović-Jovanović, Sonja and Trkulja, Vojislav and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani",
number = "2",
volume = "10",
doi = "10.3390/plants10020337"
}

Mitrović, P., Đalović, I., Kiprovski, B., Veljović-Jovanović, S., Trkulja, V., Jelušić, A.,& Popović, T.. (2021). Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel
MDPI, Basel., 10(2).
https://doi.org/10.3390/plants10020337

Mitrović P, Đalović I, Kiprovski B, Veljović-Jovanović S, Trkulja V, Jelušić A, Popović T. Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel. 2021;10(2).
doi:10.3390/plants10020337 .

Mitrović, Petar, Đalović, Ivica, Kiprovski, Biljana, Veljović-Jovanović, Sonja, Trkulja, Vojislav, Jelušić, Aleksandra, Popović, Tatjana, "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani" in Plants-Basel, 10, no. 2 (2021),
https://doi.org/10.3390/plants10020337 . .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/790
AB  - In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123
T1  - First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia
ER  - 

@conference{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra",
year = "2020",
abstract = "In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123",
title = "First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia"
}

Marković, S., Popović Milovanović, T., Mitrović, P., Iličić, R.,& Jelušić, A.. (2020). First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia. in 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini..

Marković S, Popović Milovanović T, Mitrović P, Iličić R, Jelušić A. First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia. in 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123. 2020;..

Marković, Sanja, Popović Milovanović, Tatjana, Mitrović, Petar, Iličić, Renata, Jelušić, Aleksandra, "First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia" in 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123 (2020).