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dc.creatorBulajić, Aleksandra
dc.creatorĐekić, Ivana
dc.creatorJović, Jelena
dc.creatorKrnjajić, Slobodan
dc.creatorVučurović, Ana
dc.creatorKrstić, Branka
dc.date.accessioned2019-04-04T15:53:08Z
dc.date.available2019-04-04T15:53:08Z
dc.date.issued2009
dc.identifier.issn0191-2917
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/101
dc.description.abstractIn a survey to determine the presence and distribution of It-is yellows, spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Backa District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-Iike symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB front the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.en
dc.publisherAmer Phytopathological Soc, St Paul
dc.relationMinistry of Agriculture, Forestry and Water Management, Republic of Serbia - 321-0009/2005-11
dc.rightsrestrictedAccess
dc.sourcePlant Disease
dc.titleIncidence and Distribution of Iris yellow spot virus on Onion in Serbiaen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage982
dc.citation.issue10
dc.citation.other93(10): 976-982
dc.citation.rankM21
dc.citation.spage976
dc.citation.volume93
dc.type.versionpublishedVersion
dc.identifier.doi10.1094/PDIS-93-10-0976
dc.identifier.pmid30754373
dc.identifier.rcubconv_755
dc.identifier.scopus2-s2.0-70349760037
dc.identifier.wos000270266400001


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