Antagonistic interactions between rhizobacteria and potato cyst nematodes

Abstract

The increased presence of pesticides in food chain and its consequences resulted in the development of biological agents as alternative methods of control invasive organisms. Rhizobacteria and nematodes are usual inhabitants of a soil biological network. Recent studies have shown that some rhizobacteria can act as nematode antagonists. The investigations were aimed to show a possible suppressive impact of some rhizobacteria - components of comercial microbiological manures on potato cyst nematode hatching in in vitro experiments. Effects of the three rhizobacterial species: Rhizobium leguminosarum bv. viciae, Rhizobium phaseoli and Bradyrhizobium japonicum (commercial products with sterile peat as medium) were observed on the hatching of G. rostochiensis. After 15 days of the beginning of the experiment, the percentage of hatched G. rostochiensis juveniles was counted. The hatching of juveniles in all rhizobacterial suspensions was reduced in comparison to control. The suspension of Bradyrhizobium japonicum caused greater inhibitory effect on the hatching of G. rostochiensis followed by Rhizobium leguminosarum. The impact of Rhizobium phaseoli on G. rostochiensis hatching was the least. In the similar experiment with G. pallida the supression of hatching was noticed as well. In our experiments, all the species of rhizobacteria used, decrease hatching of Globodera second stage juveniles. The antagonistic activity of these rhizobacteria on potato cyst nematodes as a potential tool for nematode biocontrol, requires further investigations.

U cilju utvrđivanja antagonista krompirovih cistolikih nematoda, kao najznačajnijih parazita krompira, ispitivan je uticaj određenih rizobakterija koje predstavljaju komponente mikrobioloških đubriva na piljenje invazivnih larvi Globodera. Rezultati ogleda in vitro ukazuju na potencijalnu mogućnost korišćenja rizobakterija u biološkoj kontroli cistolikih nematoda krompira.