Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.
Keywords:
Common bacterial blight / detection / identification / seed-borne
Source:
African Journal of Agricultural Research, 2010, 5, 19, 2730-2736
TY - JOUR
AU - Popović, Tatjana
AU - Balaž, Jelica
AU - Nikolić, Zorica
AU - Starović, Mira
AU - Gavrilović, Veljko
AU - Aleksić, Goran
AU - Vasić, Mirjana
AU - Živković, Svetlana
PY - 2010
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/123
AB - Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.
PB - Academic Journals, Victoria Island
T2 - African Journal of Agricultural Research
T1 - Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia
EP - 2736
IS - 19
SP - 2730
VL - 5
ER -
@article{
author = "Popović, Tatjana and Balaž, Jelica and Nikolić, Zorica and Starović, Mira and Gavrilović, Veljko and Aleksić, Goran and Vasić, Mirjana and Živković, Svetlana",
year = "2010",
abstract = "Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.",
publisher = "Academic Journals, Victoria Island",
journal = "African Journal of Agricultural Research",
title = "Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia",
pages = "2736-2730",
number = "19",
volume = "5"
}
Popović, T., Balaž, J., Nikolić, Z., Starović, M., Gavrilović, V., Aleksić, G., Vasić, M.,& Živković, S.. (2010). Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia. in African Journal of Agricultural Research
Academic Journals, Victoria Island., 5(19), 2730-2736.
Popović T, Balaž J, Nikolić Z, Starović M, Gavrilović V, Aleksić G, Vasić M, Živković S. Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia. in African Journal of Agricultural Research. 2010;5(19):2730-2736..
Popović, Tatjana, Balaž, Jelica, Nikolić, Zorica, Starović, Mira, Gavrilović, Veljko, Aleksić, Goran, Vasić, Mirjana, Živković, Svetlana, "Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia" in African Journal of Agricultural Research, 5, no. 19 (2010):2730-2736.
