Chlorophyll as indicator of plants response to herbicide

Abstract

Chlorophyll is a green pigment important in photosynthesis. By monitoring chlorophyll amount we learn about productivity of photosynthesis. Many herbicides with different modes of action affect the amount of chlorophyll in plant tissue. In our study we examined the amount of chlorophyll in corn plants after application of glyphosate trimesium (sulfosate). We used a non-destructive method: SPAD reading and chlorophyll a fluorescence, and a destructive method: extraction of chlorophyll with dimethil formamide and methanol. Our results show that based on amount of photosynthesis pigments and chlorophyll fluorescence yield we can monitor the stress effect of herbicides on corn plants. Our results also show that we can differentiate between treated and non treated plants.

Hlorofil je zeleni pigment važan za proces fotosinteze, pa praćenjem njegovog sadržaja u biljkama dobijamo odgovor o produktivnosti fotosinteze. Herbicidi različitih hemijskih grupa i mehanizama dejstva utiču na sadržaj hlorofila u biljnom tkivu. Ispitivan je sadržaj hlorofila u tkivu biljaka kukuruza nakon primene glifosat trimezijum (sulfosat). Primenjene su nedestruktivne metode: SPAD očitavanje sadržaja i merenje fluorescencije hlorofila a, kao i destruktivne: ekstrakcija hlorofila dimetilformamidom i metanolom. Dobijeni rezultati i izvedena istraživanja pokazuju da se na osnovu sadržaja fotosintetskih pigmenata i prinosa fluorescencije hlorofila može pratiti stresni efekat herbicida na gajene biljke kukuruza, kao i definisati nivo razlika između tretiranih i netretiranih biljaka.