Isolation and cultivation of coccoid Cyanobacteria from aerophytic biofilm in Stopić cave
Authors
Popović, SlađanaNikolić, Nataša
Savković, Željko
Stupar, Miloš
Predojević, Dragana
Anđelković, Ana
Jakovljević, Olga
Conference object (Published version)
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Cyanobacteria, characterized by unique physiological and biochemical mechanisms to withstand
harsh conditions in different habitats, can thrive in a variety of environments, including extreme ones
such as illuminated parts of caves. The information on diversity, biology and ecology of aerophytic
Cyanobacteria, especially those from cave habitats, is very limited, which is especially true for the
coccoid forms. Phototrophic biofilm was collected from the entrance zone of Stopić Cave to isolate
coccoid Cyanobacteria. The biofilm was inoculated in situ into test tubes containing solid BG11
growth medium, after which the test tubes were kept in a growth chamber under controlled conditions.
From the initial culture, which contained multiple cyanobacterial species, subsamples were extracted
and transferred to Petri dishes. These were kept under optimal conditions until a pure cultures were
obtained. The representative of the coccoid Cyanobacteria, Chroococcus, was isolated and its
...characteristics best matched the original description of Chroococcus turgidus.
Keywords:
phototrophic biofilms / cave habitats / algal cultures / Cyanobacteria / ChroococcusSource:
31st IINTERNATIONAL Conference Ecological Truth & Environmental Research 18-21 -June,2024, Sokobanja, Serbia, 2024, 621-625Publisher:
- University of Belgrade, Technical Faculty in Bor
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-MESTD-inst-2020-200010)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
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Institution/Community
IZBISTY - CONF AU - Popović, Slađana AU - Nikolić, Nataša AU - Savković, Željko AU - Stupar, Miloš AU - Predojević, Dragana AU - Anđelković, Ana AU - Jakovljević, Olga PY - 2024 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1316 AB - Cyanobacteria, characterized by unique physiological and biochemical mechanisms to withstand harsh conditions in different habitats, can thrive in a variety of environments, including extreme ones such as illuminated parts of caves. The information on diversity, biology and ecology of aerophytic Cyanobacteria, especially those from cave habitats, is very limited, which is especially true for the coccoid forms. Phototrophic biofilm was collected from the entrance zone of Stopić Cave to isolate coccoid Cyanobacteria. The biofilm was inoculated in situ into test tubes containing solid BG11 growth medium, after which the test tubes were kept in a growth chamber under controlled conditions. From the initial culture, which contained multiple cyanobacterial species, subsamples were extracted and transferred to Petri dishes. These were kept under optimal conditions until a pure cultures were obtained. The representative of the coccoid Cyanobacteria, Chroococcus, was isolated and its characteristics best matched the original description of Chroococcus turgidus. PB - University of Belgrade, Technical Faculty in Bor C3 - 31st IINTERNATIONAL Conference Ecological Truth & Environmental Research 18-21 -June,2024, Sokobanja, Serbia T1 - Isolation and cultivation of coccoid Cyanobacteria from aerophytic biofilm in Stopić cave EP - 625 SP - 621 ER -
@conference{ author = "Popović, Slađana and Nikolić, Nataša and Savković, Željko and Stupar, Miloš and Predojević, Dragana and Anđelković, Ana and Jakovljević, Olga", year = "2024", abstract = "Cyanobacteria, characterized by unique physiological and biochemical mechanisms to withstand harsh conditions in different habitats, can thrive in a variety of environments, including extreme ones such as illuminated parts of caves. The information on diversity, biology and ecology of aerophytic Cyanobacteria, especially those from cave habitats, is very limited, which is especially true for the coccoid forms. Phototrophic biofilm was collected from the entrance zone of Stopić Cave to isolate coccoid Cyanobacteria. The biofilm was inoculated in situ into test tubes containing solid BG11 growth medium, after which the test tubes were kept in a growth chamber under controlled conditions. From the initial culture, which contained multiple cyanobacterial species, subsamples were extracted and transferred to Petri dishes. These were kept under optimal conditions until a pure cultures were obtained. The representative of the coccoid Cyanobacteria, Chroococcus, was isolated and its characteristics best matched the original description of Chroococcus turgidus.", publisher = "University of Belgrade, Technical Faculty in Bor", journal = "31st IINTERNATIONAL Conference Ecological Truth & Environmental Research 18-21 -June,2024, Sokobanja, Serbia", title = "Isolation and cultivation of coccoid Cyanobacteria from aerophytic biofilm in Stopić cave", pages = "625-621" }
Popović, S., Nikolić, N., Savković, Ž., Stupar, M., Predojević, D., Anđelković, A.,& Jakovljević, O.. (2024). Isolation and cultivation of coccoid Cyanobacteria from aerophytic biofilm in Stopić cave. in 31st IINTERNATIONAL Conference Ecological Truth & Environmental Research 18-21 -June,2024, Sokobanja, Serbia University of Belgrade, Technical Faculty in Bor., 621-625.
Popović S, Nikolić N, Savković Ž, Stupar M, Predojević D, Anđelković A, Jakovljević O. Isolation and cultivation of coccoid Cyanobacteria from aerophytic biofilm in Stopić cave. in 31st IINTERNATIONAL Conference Ecological Truth & Environmental Research 18-21 -June,2024, Sokobanja, Serbia. 2024;:621-625..
Popović, Slađana, Nikolić, Nataša, Savković, Željko, Stupar, Miloš, Predojević, Dragana, Anđelković, Ana, Jakovljević, Olga, "Isolation and cultivation of coccoid Cyanobacteria from aerophytic biofilm in Stopić cave" in 31st IINTERNATIONAL Conference Ecological Truth & Environmental Research 18-21 -June,2024, Sokobanja, Serbia (2024):621-625.