DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv. campestris ISOLATES ORIGINATED FROM CABBAGE
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cabbage (Brassica o/eracea var. capitata L.) is one of the most important vegetable crop in Se, -bia, mainly significant for its richness in beneficial nutrients, both minerals and vitamins. Its yield can nowadays be reduced due to presence of many phytopathogen s. Xanthomonas campestris pv. camp estris (Xcc), a causal agent of black rot disease, is among the most significant bacteria which affect, proper cabbage growth, leading to loss in head weight and quality, therefore drastically reducing its economic value. This pathogen is genetically diverse, which can be seen through the worldwide presence of eleven races and more than thirty combinations of allelic profiles of just four housekeeping genes - dnaK, fyuA, gyrB, rpoD. The main goal of this work was to subject Xcc isolates originatecJ from cabbage to sequence analysis and to determine their affiliation to certain allelic group.
Forty-four Xcc isolates obtained from cabbage in 2014, from five different localities in Serbia (Fut...og, Temerin, $abac, Vrnjacka Banja, C:acak), were taken for analysis. DNA of all isolates was firs· amplified using BOXA 1 R rep-PCR primer to check if differences in profiles exist. Representative isolates were then sequenced on the basis of four housekeeping genes (dnaK, fyuA, gyrB, rpoD). Non-redundatant database (NRDB) from pubMLST (https://pubmlst.org/analysis/nrdb.shtml) was used to determine the allelic profiles for each of the sequenced genes, and to compare tested isolates witt already described and deposited strains from NCBI database, whose allelic profiles are known.
The results of rep-PCR fingerprinting revealed the same banding patterns for all tested isolates The allelic profile was 1, 3, 1, 1 for dnaK, fyuA, gyrB and rpoD gene, respectively. This profile coincides with Portuguese B. oleracea Xcc strains CPBF 213, CPBF 1126 (race 6) and CPBF 331 (race 10). The obtained allelic profile is assigned as sequence type 2 (ST2). In conclusion, since different races can have same allelic profile combinations and vice versa, Xcc race affiliation cannot be determined based on their genetic differences.
Извор:
VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia, 2019, 186-Издавач:
- Društvo za zaštitu bilja
Финансирање / пројекти:
- info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IR or III)/43010/RS// (RS-MESTD-Integrated and Interdisciplinary Research (IR or III)-43010)
Институција/група
IZBISTY - CONF AU - Popović, Tatjana AU - Jelušić, Aleksandra AU - Mitrović, Petar AU - Iličić, Renata AU - Marković, Sanja PY - 2019 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1343 AB - Cabbage (Brassica o/eracea var. capitata L.) is one of the most important vegetable crop in Se, -bia, mainly significant for its richness in beneficial nutrients, both minerals and vitamins. Its yield can nowadays be reduced due to presence of many phytopathogen s. Xanthomonas campestris pv. camp estris (Xcc), a causal agent of black rot disease, is among the most significant bacteria which affect, proper cabbage growth, leading to loss in head weight and quality, therefore drastically reducing its economic value. This pathogen is genetically diverse, which can be seen through the worldwide presence of eleven races and more than thirty combinations of allelic profiles of just four housekeeping genes - dnaK, fyuA, gyrB, rpoD. The main goal of this work was to subject Xcc isolates originatecJ from cabbage to sequence analysis and to determine their affiliation to certain allelic group. Forty-four Xcc isolates obtained from cabbage in 2014, from five different localities in Serbia (Futog, Temerin, $abac, Vrnjacka Banja, C:acak), were taken for analysis. DNA of all isolates was firs· amplified using BOXA 1 R rep-PCR primer to check if differences in profiles exist. Representative isolates were then sequenced on the basis of four housekeeping genes (dnaK, fyuA, gyrB, rpoD). Non-redundatant database (NRDB) from pubMLST (https://pubmlst.org/analysis/nrdb.shtml) was used to determine the allelic profiles for each of the sequenced genes, and to compare tested isolates witt already described and deposited strains from NCBI database, whose allelic profiles are known. The results of rep-PCR fingerprinting revealed the same banding patterns for all tested isolates The allelic profile was 1, 3, 1, 1 for dnaK, fyuA, gyrB and rpoD gene, respectively. This profile coincides with Portuguese B. oleracea Xcc strains CPBF 213, CPBF 1126 (race 6) and CPBF 331 (race 10). The obtained allelic profile is assigned as sequence type 2 (ST2). In conclusion, since different races can have same allelic profile combinations and vice versa, Xcc race affiliation cannot be determined based on their genetic differences. PB - Društvo za zaštitu bilja C3 - VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia T1 - DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv. campestris ISOLATES ORIGINATED FROM CABBAGE SP - 186 ER -
@conference{ author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja", year = "2019", abstract = "Cabbage (Brassica o/eracea var. capitata L.) is one of the most important vegetable crop in Se, -bia, mainly significant for its richness in beneficial nutrients, both minerals and vitamins. Its yield can nowadays be reduced due to presence of many phytopathogen s. Xanthomonas campestris pv. camp estris (Xcc), a causal agent of black rot disease, is among the most significant bacteria which affect, proper cabbage growth, leading to loss in head weight and quality, therefore drastically reducing its economic value. This pathogen is genetically diverse, which can be seen through the worldwide presence of eleven races and more than thirty combinations of allelic profiles of just four housekeeping genes - dnaK, fyuA, gyrB, rpoD. The main goal of this work was to subject Xcc isolates originatecJ from cabbage to sequence analysis and to determine their affiliation to certain allelic group. Forty-four Xcc isolates obtained from cabbage in 2014, from five different localities in Serbia (Futog, Temerin, $abac, Vrnjacka Banja, C:acak), were taken for analysis. DNA of all isolates was firs· amplified using BOXA 1 R rep-PCR primer to check if differences in profiles exist. Representative isolates were then sequenced on the basis of four housekeeping genes (dnaK, fyuA, gyrB, rpoD). Non-redundatant database (NRDB) from pubMLST (https://pubmlst.org/analysis/nrdb.shtml) was used to determine the allelic profiles for each of the sequenced genes, and to compare tested isolates witt already described and deposited strains from NCBI database, whose allelic profiles are known. The results of rep-PCR fingerprinting revealed the same banding patterns for all tested isolates The allelic profile was 1, 3, 1, 1 for dnaK, fyuA, gyrB and rpoD gene, respectively. This profile coincides with Portuguese B. oleracea Xcc strains CPBF 213, CPBF 1126 (race 6) and CPBF 331 (race 10). The obtained allelic profile is assigned as sequence type 2 (ST2). In conclusion, since different races can have same allelic profile combinations and vice versa, Xcc race affiliation cannot be determined based on their genetic differences.", publisher = "Društvo za zaštitu bilja", journal = "VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia", title = "DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv. campestris ISOLATES ORIGINATED FROM CABBAGE", pages = "186" }
Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2019). DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv. campestris ISOLATES ORIGINATED FROM CABBAGE. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia Društvo za zaštitu bilja., 186.
Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv. campestris ISOLATES ORIGINATED FROM CABBAGE. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia. 2019;:186..
Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "DETERMINATION OF ALLELIC PROFILE OF Xanthomonas campestris pv. campestris ISOLATES ORIGINATED FROM CABBAGE" in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia (2019):186.