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Morfološka i molekularna identifikacija populacija cistolikih nematoda krompira u Srbiji

dc.creatorOro, Violeta
dc.creatorIvanović, Žarko
dc.creatorNikolić, Bogdan
dc.creatorBarši, L.
dc.creatorRadivojević, M.
dc.creatorJovčić, B.
dc.date.accessioned2019-04-04T15:54:25Z
dc.date.available2019-04-04T15:54:25Z
dc.date.issued2010
dc.identifier.issn0354-4664
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/143
dc.description.abstractQuarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day.en
dc.description.abstractKarantinske vrste kao što su cistolike nematode krompira Globodera rostochiensis i G. pallida su prisutne u Srbiji od 1999. god. odn. 2005. god. Ove nematode su sestrinske vrste i njihova morfološka identifikacija je složena zbog morfometrijskog preklapanja. Ciste sa lokaliteta Kladnica, Šanac, Gojna Gora i Milatovići su gajene na osetljivim sortama krompira i njihove morfološke razlike su analizirane. Da bi se izbegle nejasnoće u morfološkom određivanju vrsta, izabran je duplex PCR metod za brzu i preciznu specijsku identifikaciju. Celokupna procedura, od ekstrakcije DNK do njene vizualizacije, može biti urađena za jedan dan.sr
dc.publisherUniversity of Belgrade, University of Novi Sad
dc.relationProjekat Ministarstva nauke Republike Srbije, br. 20051: Optimizacija primene hemijskih sredstava u zaštiti bilja povećanjem efikasnosti dijagnostičkih metoda i procene rizika pojave bolesti, štetočina i korova
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceArchives of Biological Sciences
dc.subjectpotato cyst nematodesen
dc.subjectpopulationsen
dc.subjectmorphologyen
dc.subjectduplex PCRen
dc.titleMorphological and molecular identification of potato cyst nematode populations in Serbiaen
dc.titleMorfološka i molekularna identifikacija populacija cistolikih nematoda krompira u Srbijisr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage754
dc.citation.issue3
dc.citation.other62(3): 747-754
dc.citation.rankM23
dc.citation.spage747
dc.citation.volume62
dc.type.versionpublishedVersion
dc.identifier.doi10.2298/ABS1003747O
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1310/141.pdf
dc.identifier.rcubconv_74
dc.identifier.scopus2-s2.0-77955149985
dc.identifier.wos000281482000030


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