During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first rec...ord of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia.
TY - JOUR
AU - Ivanović, Žarko
AU - Trkulja, Nenad
AU - Živković, Svetlana
AU - Pfaf-Dolovac, Erika
AU - Dolovac, Nenad
AU - Jović, Jelena
AU - Mitrović, Milana
PY - 2011
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/149
AB - During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first record of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia.
PB - Alma Mater Studiorum, Univ Bologna, Bologna
T2 - Bulletin of Insectology
T1 - First report of stolbur phytoplasma infecting celery in Serbia
VL - 64
ER -
@article{
author = "Ivanović, Žarko and Trkulja, Nenad and Živković, Svetlana and Pfaf-Dolovac, Erika and Dolovac, Nenad and Jović, Jelena and Mitrović, Milana",
year = "2011",
abstract = "During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first record of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia.",
publisher = "Alma Mater Studiorum, Univ Bologna, Bologna",
journal = "Bulletin of Insectology",
title = "First report of stolbur phytoplasma infecting celery in Serbia",
volume = "64"
}
Ivanović, Ž., Trkulja, N., Živković, S., Pfaf-Dolovac, E., Dolovac, N., Jović, J.,& Mitrović, M.. (2011). First report of stolbur phytoplasma infecting celery in Serbia. in Bulletin of Insectology
Alma Mater Studiorum, Univ Bologna, Bologna., 64.
Ivanović Ž, Trkulja N, Živković S, Pfaf-Dolovac E, Dolovac N, Jović J, Mitrović M. First report of stolbur phytoplasma infecting celery in Serbia. in Bulletin of Insectology. 2011;64..
Ivanović, Žarko, Trkulja, Nenad, Živković, Svetlana, Pfaf-Dolovac, Erika, Dolovac, Nenad, Jović, Jelena, Mitrović, Milana, "First report of stolbur phytoplasma infecting celery in Serbia" in Bulletin of Insectology, 64 (2011).
