First report of stolbur phytoplasma infecting celery in Serbia
Само за регистроване кориснике
2011
Аутори
Ivanović, ŽarkoTrkulja, Nenad
Živković, Svetlana
Pfaf-Dolovac, Erika
Dolovac, Nenad
Jović, Jelena
Mitrović, Milana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first rec...ord of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia.
Кључне речи:
Apium graveolens / PCR / RFLP / Serbia / stolbur phytoplasma / tuf geneИзвор:
Bulletin of Insectology, 2011, 64Издавач:
- Alma Mater Studiorum, Univ Bologna, Bologna
Финансирање / пројекти:
- Разрада интегрисаног управљања и примене савремених принципа сузбијања штетних организама у заштити биља (RS-MESTD-Technological Development (TD or TR)-31018)
- Агробиодиверзитет и коришћење земљишта у Србији: интегрисана процена биодиверзитета кључних група артропода и биљних патогена (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43001)
Институција/група
IZBISTY - JOUR AU - Ivanović, Žarko AU - Trkulja, Nenad AU - Živković, Svetlana AU - Pfaf-Dolovac, Erika AU - Dolovac, Nenad AU - Jović, Jelena AU - Mitrović, Milana PY - 2011 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/149 AB - During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first record of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia. PB - Alma Mater Studiorum, Univ Bologna, Bologna T2 - Bulletin of Insectology T1 - First report of stolbur phytoplasma infecting celery in Serbia VL - 64 ER -
@article{ author = "Ivanović, Žarko and Trkulja, Nenad and Živković, Svetlana and Pfaf-Dolovac, Erika and Dolovac, Nenad and Jović, Jelena and Mitrović, Milana", year = "2011", abstract = "During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first record of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia.", publisher = "Alma Mater Studiorum, Univ Bologna, Bologna", journal = "Bulletin of Insectology", title = "First report of stolbur phytoplasma infecting celery in Serbia", volume = "64" }
Ivanović, Ž., Trkulja, N., Živković, S., Pfaf-Dolovac, E., Dolovac, N., Jović, J.,& Mitrović, M.. (2011). First report of stolbur phytoplasma infecting celery in Serbia. in Bulletin of Insectology Alma Mater Studiorum, Univ Bologna, Bologna., 64.
Ivanović Ž, Trkulja N, Živković S, Pfaf-Dolovac E, Dolovac N, Jović J, Mitrović M. First report of stolbur phytoplasma infecting celery in Serbia. in Bulletin of Insectology. 2011;64..
Ivanović, Žarko, Trkulja, Nenad, Živković, Svetlana, Pfaf-Dolovac, Erika, Dolovac, Nenad, Jović, Jelena, Mitrović, Milana, "First report of stolbur phytoplasma infecting celery in Serbia" in Bulletin of Insectology, 64 (2011).