Marshmallow is a host of a number of saprophytic and parasitic fungi in Serbia. The seeds of marshmallow are contaminated with fungi from different genera, especially Alternaria and Fusarium, which significantly reduced seed germination and caused seedling decay. In this study we investigate antagnonism of indigenous Bacillus sp. isolate Q3 against marshmallow mycopopulation. Bacillus sp. Q3 was isolated from maize rhizosphere, characterized by polyphasic approch and tested for plant growth promoting treats. Bacillus sp. Q3 produced antifungal metabolites with growth inhibition activity against numerous fungi in dual culture: 61.8% of Alternaria alternata, 74.8% of Myrothecium verrucaria and 33.6% of Sclerotinia sclerotiorum. That effect could be caused by different antifungal metabolites including siderophores, hydrolytic enzymes, organic acids and indole acetic acid (IAA). Suppression of natural marshmallow seed infection by Q3 isolate was observed. The seeds were immersed in differe...nt concentrations of bacterial suspension during 2h and their infections by phytopathogenic fungi were estimated. The results showed significant reduction of seed infection by Alternaria spp. The presented results indicate possible application of this isolate as promising biological agent for control of marshmallow seed pathogenic fungi.
Beli slez gajen u Srbiji je domaćin mnogim saprofitnim i parazitskim gljivama. Seme je zaraženo gljivama iz različitih rodova, naročito Alternaria i Fusarium, ko je značajno redukuju klijavost semena i izazivaju sušenje. U ovom radu ispitivan je antagonizam autohtone bakterije Bacillus sp. izolata Q3 i mikopopulacije belog sleza. Bacillus sp. Q3 je izolovan iz rizosfere kukuruza, karakterizacija je izvršena polifaznom metodologijom i testirane su osobine odgovorne za stimulaciju rasta biljaka. Bacillus sp. Q3 produkuje antifungalne metabolite sa izraženom aktivnošću protiv fitopatogenih gljiva sa različitim procentom inhibicije rasta u dvojnoj kulturi: 61.75% kod Alternaria alternata, 74.80% kod Myrothecium verrucaria i 33.63% kod Sclerotinia sclerotiorum. Ovaj efekat je posledica produkcije različitih antifungalnih metabolita, uključujući siderofore, hidrolitičke enzime, organske kiseline i indolsirćetnu kiselinu (IAA). Ustanovljeno je suzbijanje prirodne infekcije semena belog sleza ...primenom izolata Bacillus Q3. Semena su potapana u različite koncentracije bakterijske suspenzije tokom 2 h i praćen je stepen infekcije fitopatogenim gljivama. Rezultati su pokazali značajan stepen redukcije infekcije semena belog sleza gljivom Alternaria spp. Ovi rezultati ukazuju na moguću primenu ovog izolata kao potentnog biološkog agensa za kontrolu infekcije semena belog sleza fitopatogenim gljivama.
TY - JOUR
AU - Jošić, Dragana
AU - Pivić, Radmila
AU - Pavlović, Snežana
AU - Stojanović, Saša
AU - Aleksić, Goran
AU - Starović, Mira
PY - 2011
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/184
AB - Marshmallow is a host of a number of saprophytic and parasitic fungi in Serbia. The seeds of marshmallow are contaminated with fungi from different genera, especially Alternaria and Fusarium, which significantly reduced seed germination and caused seedling decay. In this study we investigate antagnonism of indigenous Bacillus sp. isolate Q3 against marshmallow mycopopulation. Bacillus sp. Q3 was isolated from maize rhizosphere, characterized by polyphasic approch and tested for plant growth promoting treats. Bacillus sp. Q3 produced antifungal metabolites with growth inhibition activity against numerous fungi in dual culture: 61.8% of Alternaria alternata, 74.8% of Myrothecium verrucaria and 33.6% of Sclerotinia sclerotiorum. That effect could be caused by different antifungal metabolites including siderophores, hydrolytic enzymes, organic acids and indole acetic acid (IAA). Suppression of natural marshmallow seed infection by Q3 isolate was observed. The seeds were immersed in different concentrations of bacterial suspension during 2h and their infections by phytopathogenic fungi were estimated. The results showed significant reduction of seed infection by Alternaria spp. The presented results indicate possible application of this isolate as promising biological agent for control of marshmallow seed pathogenic fungi.
AB - Beli slez gajen u Srbiji je domaćin mnogim saprofitnim i parazitskim gljivama. Seme je zaraženo gljivama iz različitih rodova, naročito Alternaria i Fusarium, ko je značajno redukuju klijavost semena i izazivaju sušenje. U ovom radu ispitivan je antagonizam autohtone bakterije Bacillus sp. izolata Q3 i mikopopulacije belog sleza. Bacillus sp. Q3 je izolovan iz rizosfere kukuruza, karakterizacija je izvršena polifaznom metodologijom i testirane su osobine odgovorne za stimulaciju rasta biljaka. Bacillus sp. Q3 produkuje antifungalne metabolite sa izraženom aktivnošću protiv fitopatogenih gljiva sa različitim procentom inhibicije rasta u dvojnoj kulturi: 61.75% kod Alternaria alternata, 74.80% kod Myrothecium verrucaria i 33.63% kod Sclerotinia sclerotiorum. Ovaj efekat je posledica produkcije različitih antifungalnih metabolita, uključujući siderofore, hidrolitičke enzime, organske kiseline i indolsirćetnu kiselinu (IAA). Ustanovljeno je suzbijanje prirodne infekcije semena belog sleza primenom izolata Bacillus Q3. Semena su potapana u različite koncentracije bakterijske suspenzije tokom 2 h i praćen je stepen infekcije fitopatogenim gljivama. Rezultati su pokazali značajan stepen redukcije infekcije semena belog sleza gljivom Alternaria spp. Ovi rezultati ukazuju na moguću primenu ovog izolata kao potentnog biološkog agensa za kontrolu infekcije semena belog sleza fitopatogenim gljivama.
PB - Matica srpska, Novi Sad
T2 - Zbornik Matice srpske za prirodne nauke
T1 - Antifungal activity of indigenous bacillus sp. isolate Q3 against marshmallow mycobiota
T1 - Antifungalna aktivnost autohtonog izolata Bacillus sp. Q3 na mikopopulaciju belog sleza
EP - 120
IS - 120
SP - 111
DO - 10.2298/ZMSPN1120111J
ER -
@article{
author = "Jošić, Dragana and Pivić, Radmila and Pavlović, Snežana and Stojanović, Saša and Aleksić, Goran and Starović, Mira",
year = "2011",
abstract = "Marshmallow is a host of a number of saprophytic and parasitic fungi in Serbia. The seeds of marshmallow are contaminated with fungi from different genera, especially Alternaria and Fusarium, which significantly reduced seed germination and caused seedling decay. In this study we investigate antagnonism of indigenous Bacillus sp. isolate Q3 against marshmallow mycopopulation. Bacillus sp. Q3 was isolated from maize rhizosphere, characterized by polyphasic approch and tested for plant growth promoting treats. Bacillus sp. Q3 produced antifungal metabolites with growth inhibition activity against numerous fungi in dual culture: 61.8% of Alternaria alternata, 74.8% of Myrothecium verrucaria and 33.6% of Sclerotinia sclerotiorum. That effect could be caused by different antifungal metabolites including siderophores, hydrolytic enzymes, organic acids and indole acetic acid (IAA). Suppression of natural marshmallow seed infection by Q3 isolate was observed. The seeds were immersed in different concentrations of bacterial suspension during 2h and their infections by phytopathogenic fungi were estimated. The results showed significant reduction of seed infection by Alternaria spp. The presented results indicate possible application of this isolate as promising biological agent for control of marshmallow seed pathogenic fungi., Beli slez gajen u Srbiji je domaćin mnogim saprofitnim i parazitskim gljivama. Seme je zaraženo gljivama iz različitih rodova, naročito Alternaria i Fusarium, ko je značajno redukuju klijavost semena i izazivaju sušenje. U ovom radu ispitivan je antagonizam autohtone bakterije Bacillus sp. izolata Q3 i mikopopulacije belog sleza. Bacillus sp. Q3 je izolovan iz rizosfere kukuruza, karakterizacija je izvršena polifaznom metodologijom i testirane su osobine odgovorne za stimulaciju rasta biljaka. Bacillus sp. Q3 produkuje antifungalne metabolite sa izraženom aktivnošću protiv fitopatogenih gljiva sa različitim procentom inhibicije rasta u dvojnoj kulturi: 61.75% kod Alternaria alternata, 74.80% kod Myrothecium verrucaria i 33.63% kod Sclerotinia sclerotiorum. Ovaj efekat je posledica produkcije različitih antifungalnih metabolita, uključujući siderofore, hidrolitičke enzime, organske kiseline i indolsirćetnu kiselinu (IAA). Ustanovljeno je suzbijanje prirodne infekcije semena belog sleza primenom izolata Bacillus Q3. Semena su potapana u različite koncentracije bakterijske suspenzije tokom 2 h i praćen je stepen infekcije fitopatogenim gljivama. Rezultati su pokazali značajan stepen redukcije infekcije semena belog sleza gljivom Alternaria spp. Ovi rezultati ukazuju na moguću primenu ovog izolata kao potentnog biološkog agensa za kontrolu infekcije semena belog sleza fitopatogenim gljivama.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of indigenous bacillus sp. isolate Q3 against marshmallow mycobiota, Antifungalna aktivnost autohtonog izolata Bacillus sp. Q3 na mikopopulaciju belog sleza",
pages = "120-111",
number = "120",
doi = "10.2298/ZMSPN1120111J"
}
Jošić, D., Pivić, R., Pavlović, S., Stojanović, S., Aleksić, G.,& Starović, M.. (2011). Antifungal activity of indigenous bacillus sp. isolate Q3 against marshmallow mycobiota. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(120), 111-120.
https://doi.org/10.2298/ZMSPN1120111J
Jošić D, Pivić R, Pavlović S, Stojanović S, Aleksić G, Starović M. Antifungal activity of indigenous bacillus sp. isolate Q3 against marshmallow mycobiota. in Zbornik Matice srpske za prirodne nauke. 2011;(120):111-120.
doi:10.2298/ZMSPN1120111J .
Jošić, Dragana, Pivić, Radmila, Pavlović, Snežana, Stojanović, Saša, Aleksić, Goran, Starović, Mira, "Antifungal activity of indigenous bacillus sp. isolate Q3 against marshmallow mycobiota" in Zbornik Matice srpske za prirodne nauke, no. 120 (2011):111-120,
https://doi.org/10.2298/ZMSPN1120111J . .
