Detection and Identification of Two Phytoplasmas (16SrIII-B and 16SrXII-A) From Alfalfa (Medicago sativa) in Serbia
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Plants of alfalfa (Medicago sativa) exhibiting general stunting, proliferation and phyllody associated with leaf yellowing and reddening were observed in three localities of Central Serbia. Phytoplasma strains belonging to 16SrIII-B and 16SrXII-A groups were detected and identified by RFLP and sequence analysis of 16S rDNA. Stolbur phytoplasma tuf gene RFLP analysis showed the presence of the TufAY-b-type phytoplasma subgroup in 80% of symptomatic samples. This is the first report of 16SrIII-B and 16SrXII-A phytoplasma groups affecting alfalfa in Serbia.
Keywords:alfalfa / RFLP / 16SrIII-B phytoplasma / stolbur / TufAY-b type phytoplasma / Serbia
Source:Journal of Phytopathology, 2012, 160, 11-12, 758-760
- Wiley-Blackwell, Hoboken