In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. The strains were identified as Agrobacterium vitis using differential physiological and biochemical tests, and a multiplex PCR assay targeting 23S rRNA gene sequences. In the pathogenicity assay, all strains induced characteristic symptoms on inoculated tomato and grapevine plants. They were less virulent on tomato plants in comparison to the reference strains of A. tumefaciens and A. vitis.
TY - JOUR
AU - Kuzmanović, Nemanja
AU - Gašić, Katarina
AU - Ivanović, Milan M
AU - Prokić, Anđelka
AU - Obradović, Aleksa
PY - 2012
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/201
AB - In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. The strains were identified as Agrobacterium vitis using differential physiological and biochemical tests, and a multiplex PCR assay targeting 23S rRNA gene sequences. In the pathogenicity assay, all strains induced characteristic symptoms on inoculated tomato and grapevine plants. They were less virulent on tomato plants in comparison to the reference strains of A. tumefaciens and A. vitis.
PB - University of Belgrade, University of Novi Sad
T2 - Archives of Biological Sciences
T1 - Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia
EP - 1494
IS - 4
SP - 1487
VL - 64
DO - 10.2298/ABS1204487K
ER -
@article{
author = "Kuzmanović, Nemanja and Gašić, Katarina and Ivanović, Milan M and Prokić, Anđelka and Obradović, Aleksa",
year = "2012",
abstract = "In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. The strains were identified as Agrobacterium vitis using differential physiological and biochemical tests, and a multiplex PCR assay targeting 23S rRNA gene sequences. In the pathogenicity assay, all strains induced characteristic symptoms on inoculated tomato and grapevine plants. They were less virulent on tomato plants in comparison to the reference strains of A. tumefaciens and A. vitis.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia",
pages = "1494-1487",
number = "4",
volume = "64",
doi = "10.2298/ABS1204487K"
}
Kuzmanović, N., Gašić, K., Ivanović, M. M., Prokić, A.,& Obradović, A.. (2012). Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 64(4), 1487-1494.
https://doi.org/10.2298/ABS1204487K
Kuzmanović N, Gašić K, Ivanović MM, Prokić A, Obradović A. Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia. in Archives of Biological Sciences. 2012;64(4):1487-1494.
doi:10.2298/ABS1204487K .
Kuzmanović, Nemanja, Gašić, Katarina, Ivanović, Milan M, Prokić, Anđelka, Obradović, Aleksa, "Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia" in Archives of Biological Sciences, 64, no. 4 (2012):1487-1494,
https://doi.org/10.2298/ABS1204487K . .
