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Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia

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2012
199.pdf (653.7Kb)
Authors
Kuzmanović, Nemanja
Gašić, Katarina
Ivanović, Milan M
Prokić, Anđelka
Obradović, Aleksa
article (publishedVersion)
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Abstract
In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. The strains were identified as Agrobacterium vitis using differential physiological and biochemical tests, and a multiplex PCR assay targeting 23S rRNA gene sequences. In the pathogenicity assay, all strains induced characteristic symptoms on inoculated tomato and grapevine plants. They were less virulent on tomato plants in comparison to the reference strains of A. tumefaciens and A. vitis.
Keywords:
Crown gall / grapevine / Agrobacterium vitis / tumor / pathogenicity / plasmid
Source:
Archives of Biological Sciences, 2012, 64, 4, 1487-1494
Publisher:
  • University of Belgrade, University of Novi Sad
Funding / projects:
  • info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46008/RS// (RS-46008)

DOI: 10.2298/ABS1204487K

ISSN: 0354-4664

WoS: 000312046200032

Scopus: 2-s2.0-84867777241
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URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/201
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