Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia
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2012
Authors
Ivanović, Žarko
Stanković, Slaviša

Živković, Svetlana

Gavrilović, Veljko
Kojić, Milan

Fira, Đorđe

Article (Published version)

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Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations b...etween strains of P. syringae from different pome and stone fruit hosts in Serbia.
Keywords:
Pseudomonas syringae / Fruit trees / CharacterizationSource:
European Journal of Plant Pathology, 2012, 134, 1, 191-203Publisher:
- Springer, Dordrecht
Funding / projects:
- Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control (RS-173026)
- Development of integrated approach in plant protection for control harmful organisms (RS-31018)
DOI: 10.1007/s10658-012-9978-4
ISSN: 0929-1873
WoS: 000306739600017
Scopus: 2-s2.0-84864401261
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IZBISTY - JOUR AU - Ivanović, Žarko AU - Stanković, Slaviša AU - Živković, Svetlana AU - Gavrilović, Veljko AU - Kojić, Milan AU - Fira, Đorđe PY - 2012 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/208 AB - Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia. PB - Springer, Dordrecht T2 - European Journal of Plant Pathology T1 - Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia EP - 203 IS - 1 SP - 191 VL - 134 DO - 10.1007/s10658-012-9978-4 ER -
@article{ author = "Ivanović, Žarko and Stanković, Slaviša and Živković, Svetlana and Gavrilović, Veljko and Kojić, Milan and Fira, Đorđe", year = "2012", abstract = "Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.", publisher = "Springer, Dordrecht", journal = "European Journal of Plant Pathology", title = "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia", pages = "203-191", number = "1", volume = "134", doi = "10.1007/s10658-012-9978-4" }
Ivanović, Ž., Stanković, S., Živković, S., Gavrilović, V., Kojić, M.,& Fira, Đ.. (2012). Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology Springer, Dordrecht., 134(1), 191-203. https://doi.org/10.1007/s10658-012-9978-4
Ivanović Ž, Stanković S, Živković S, Gavrilović V, Kojić M, Fira Đ. Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology. 2012;134(1):191-203. doi:10.1007/s10658-012-9978-4 .
Ivanović, Žarko, Stanković, Slaviša, Živković, Svetlana, Gavrilović, Veljko, Kojić, Milan, Fira, Đorđe, "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia" in European Journal of Plant Pathology, 134, no. 1 (2012):191-203, https://doi.org/10.1007/s10658-012-9978-4 . .