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Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds

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2012
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Аутори
Popović, Tatjana
Milovanović, Predrag
Aleksić, Goran
Gavrilović, Veljko
Starović, Mira
Vasić, Mirjana
Balaž, Jelica
Чланак у часопису (Објављена верзија)
Метаподаци
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Апстракт
Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained strains was confirmed by the inoculation of germinated bean seed. The isolates recovered from the seed assay were further confirmed to be Psp by using both Enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (Nested-PCR...) detection methodologies. The International Seed Testing Association (ISTA) method selected for this work was found to be effective in detecting the presence of Psp in common bean seed. The bacterium Psp was detected in only two of the 23 seed samples analyzed by this method, which shows that the bacterium is not widespread in Serbia.

Кључне речи:
MSP / MT / ELISA / Nested-PCR / halo blight / pathogenicity
Извор:
Scientia Agricola, 2012, 69, 4, 265-270
Издавач:
  • Universidade de São Paulo

DOI: 10.1590/S0103-90162012000400005

ISSN: 0103-9016

WoS: 000306026900005

Scopus: 2-s2.0-84859348921
[ Google Scholar ]
1
1
URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/209
Колекције
  • Radovi istraživača / Researchers' publications
Институција/група
IZBIS
TY  - JOUR
AU  - Popović, Tatjana
AU  - Milovanović, Predrag
AU  - Aleksić, Goran
AU  - Gavrilović, Veljko
AU  - Starović, Mira
AU  - Vasić, Mirjana
AU  - Balaž, Jelica
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/209
AB  - Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained strains was confirmed by the inoculation of germinated bean seed. The isolates recovered from the seed assay were further confirmed to be Psp by using both Enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (Nested-PCR) detection methodologies. The International Seed Testing Association (ISTA) method selected for this work was found to be effective in detecting the presence of Psp in common bean seed. The bacterium Psp was detected in only two of the 23 seed samples analyzed by this method, which shows that the bacterium is not widespread in Serbia.
PB  - Universidade de São Paulo
T2  - Scientia Agricola
T1  - Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds
EP  - 270
IS  - 4
SP  - 265
VL  - 69
DO  - 10.1590/S0103-90162012000400005
ER  - 
@article{
author = "Popović, Tatjana and Milovanović, Predrag and Aleksić, Goran and Gavrilović, Veljko and Starović, Mira and Vasić, Mirjana and Balaž, Jelica",
year = "2012",
abstract = "Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained strains was confirmed by the inoculation of germinated bean seed. The isolates recovered from the seed assay were further confirmed to be Psp by using both Enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (Nested-PCR) detection methodologies. The International Seed Testing Association (ISTA) method selected for this work was found to be effective in detecting the presence of Psp in common bean seed. The bacterium Psp was detected in only two of the 23 seed samples analyzed by this method, which shows that the bacterium is not widespread in Serbia.",
publisher = "Universidade de São Paulo",
journal = "Scientia Agricola",
title = "Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds",
pages = "270-265",
number = "4",
volume = "69",
doi = "10.1590/S0103-90162012000400005"
}
Popović, T., Milovanović, P., Aleksić, G., Gavrilović, V., Starović, M., Vasić, M.,& Balaž, J.. (2012). Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds. in Scientia Agricola
Universidade de São Paulo., 69(4), 265-270.
https://doi.org/10.1590/S0103-90162012000400005
Popović T, Milovanović P, Aleksić G, Gavrilović V, Starović M, Vasić M, Balaž J. Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds. in Scientia Agricola. 2012;69(4):265-270.
doi:10.1590/S0103-90162012000400005 .
Popović, Tatjana, Milovanović, Predrag, Aleksić, Goran, Gavrilović, Veljko, Starović, Mira, Vasić, Mirjana, Balaž, Jelica, "Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds" in Scientia Agricola, 69, no. 4 (2012):265-270,
https://doi.org/10.1590/S0103-90162012000400005 . .

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