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dc.creatorPopović, Tatjana
dc.creatorMilovanović, Predrag
dc.creatorAleksić, Goran
dc.creatorGavrilović, Veljko
dc.creatorStarović, Mira
dc.creatorVasić, Mirjana
dc.creatorBalaž, Jelica
dc.date.accessioned2019-04-04T15:56:30Z
dc.date.available2019-04-04T15:56:30Z
dc.date.issued2012
dc.identifier.issn0103-9016
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/209
dc.description.abstractHalo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained strains was confirmed by the inoculation of germinated bean seed. The isolates recovered from the seed assay were further confirmed to be Psp by using both Enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (Nested-PCR) detection methodologies. The International Seed Testing Association (ISTA) method selected for this work was found to be effective in detecting the presence of Psp in common bean seed. The bacterium Psp was detected in only two of the 23 seed samples analyzed by this method, which shows that the bacterium is not widespread in Serbia.en
dc.publisherUniversidade de São Paulo
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScientia Agricola
dc.subjectMSPen
dc.subjectMTen
dc.subjectELISAen
dc.subjectNested-PCRen
dc.subjecthalo blighten
dc.subjectpathogenicityen
dc.titleApplication of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seedsen
dc.typearticle
dc.rights.licenseBY
dc.citation.epage270
dc.citation.issue4
dc.citation.other69(4): 265-270
dc.citation.rankM22
dc.citation.spage265
dc.citation.volume69
dc.type.versionpublishedVersion
dc.identifier.doi10.1590/S0103-90162012000400005
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1368/207.pdf
dc.identifier.rcubconv_813
dc.identifier.scopus2-s2.0-84859348921
dc.identifier.wos000306026900005


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