The pathogenicity of phytopathogenic fungi is associated with phytotoxins, especially with their chemical nature and quantity. Sirodesmins are phytotoxins from the epipolythiodioxopiperazines group, produced by the fungus Leptosphaeria maculans, which are a cause of blackleg and stem canker in oilseed rape (Brassica napus L.). The aim of this work was to obtain a detailed chemical profile of sirodesmins in five fungal isolates (four from Vojvodina, Serbia, and one from the Centre for Agricultural Research, Rothamsted, UK). Sirodesmins showing different phytotoxicity on treated cotyledons of cv. Quinta were separated and detected by thin layer chromatography in all analysed isolates (L.m, C-3, St-5 and S-11) except K-113, which neither contained sirodesmin congeners nor did it exhibit activity. By use of high performance liquid chromatography coupled with tandem mass spectrometer, it was possible to identify total of 10 sirodesmins, together with their precursor-phomamide. It was found ...that the dominant epipolythiodioxopiperazines of the investigated L. maculans isolates were sirodesmin PL, sirodesmin C, and their de-acetylated derivatives.
Patogenost fitopatogenih gljiva povezana je sa fitotoksinima, a naročito sa njihovom hemijskom prirodom i količinom. Sirodezmini su fitotoksini iz grupe epipolitiodioksopiperazina, koje proizvodi gljiva Leptosphaeria maculans, uzročnik suve truleži korena i raka stabla uljane repice. Cilj ovog rada bila je detaljna hemijska karakterizacija sirodezmina u pet izolata gljiva (četiri iz Vojvodine i jedan iz Velike Britanije, Centar za poljoprivredna istraživanja, Rothamsted). Kod svih ispitivanih izolata (L. maculans, C-3, St-3, S-11), osim K-113 (koji nije sadržao sirodezmine niti pokazivao aktivnost) tankoslojnom hromatografijom su razdvojeni i detektovani sirodezmini koji su pokazali različitu fitotoksičnost na tretiranim kotiledonima sorte Quinta. Primenom tečne hromatografije visoke efikasnosti, kuplovane sa tandemskim masenim spektrometrom, bilo je moguće identifikovati ukupno 10 sirodezmina, kao i njihov prekursor - fomamid. Utvrđeno je da su dominantni epipolitio-dioksopiperazini i...spitivanih izolata L. maculans sirodezmin PL, sirodezmin C i njihovi deacetilovani derivati.
TY - JOUR
AU - Mitrović, Petar
AU - Orčić, Dejan Z.
AU - Sakač, Zvonimir
AU - Marjanović-Jeromela, Ana
AU - Grahovac, Nada L.
AU - Milošević, Drago
AU - Marisavljević, Dragana
PY - 2012
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/210
AB - The pathogenicity of phytopathogenic fungi is associated with phytotoxins, especially with their chemical nature and quantity. Sirodesmins are phytotoxins from the epipolythiodioxopiperazines group, produced by the fungus Leptosphaeria maculans, which are a cause of blackleg and stem canker in oilseed rape (Brassica napus L.). The aim of this work was to obtain a detailed chemical profile of sirodesmins in five fungal isolates (four from Vojvodina, Serbia, and one from the Centre for Agricultural Research, Rothamsted, UK). Sirodesmins showing different phytotoxicity on treated cotyledons of cv. Quinta were separated and detected by thin layer chromatography in all analysed isolates (L.m, C-3, St-5 and S-11) except K-113, which neither contained sirodesmin congeners nor did it exhibit activity. By use of high performance liquid chromatography coupled with tandem mass spectrometer, it was possible to identify total of 10 sirodesmins, together with their precursor-phomamide. It was found that the dominant epipolythiodioxopiperazines of the investigated L. maculans isolates were sirodesmin PL, sirodesmin C, and their de-acetylated derivatives.
AB - Patogenost fitopatogenih gljiva povezana je sa fitotoksinima, a naročito sa njihovom hemijskom prirodom i količinom. Sirodezmini su fitotoksini iz grupe epipolitiodioksopiperazina, koje proizvodi gljiva Leptosphaeria maculans, uzročnik suve truleži korena i raka stabla uljane repice. Cilj ovog rada bila je detaljna hemijska karakterizacija sirodezmina u pet izolata gljiva (četiri iz Vojvodine i jedan iz Velike Britanije, Centar za poljoprivredna istraživanja, Rothamsted). Kod svih ispitivanih izolata (L. maculans, C-3, St-3, S-11), osim K-113 (koji nije sadržao sirodezmine niti pokazivao aktivnost) tankoslojnom hromatografijom su razdvojeni i detektovani sirodezmini koji su pokazali različitu fitotoksičnost na tretiranim kotiledonima sorte Quinta. Primenom tečne hromatografije visoke efikasnosti, kuplovane sa tandemskim masenim spektrometrom, bilo je moguće identifikovati ukupno 10 sirodezmina, kao i njihov prekursor - fomamid. Utvrđeno je da su dominantni epipolitio-dioksopiperazini ispitivanih izolata L. maculans sirodezmin PL, sirodezmin C i njihovi deacetilovani derivati.
PB - Serbian Chemical Society, Belgrade
T2 - Journal of the Serbian Chemical Society
T1 - Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans
T1 - Karakterizacija sirodezmina izolovanih iz fitopatogene gljive Leptosphaeria maculans
EP - 1379
IS - 10
SP - 1363
VL - 77
DO - 10.2298/JSC111231048M
UR - conv_21
ER -
@article{
author = "Mitrović, Petar and Orčić, Dejan Z. and Sakač, Zvonimir and Marjanović-Jeromela, Ana and Grahovac, Nada L. and Milošević, Drago and Marisavljević, Dragana",
year = "2012",
abstract = "The pathogenicity of phytopathogenic fungi is associated with phytotoxins, especially with their chemical nature and quantity. Sirodesmins are phytotoxins from the epipolythiodioxopiperazines group, produced by the fungus Leptosphaeria maculans, which are a cause of blackleg and stem canker in oilseed rape (Brassica napus L.). The aim of this work was to obtain a detailed chemical profile of sirodesmins in five fungal isolates (four from Vojvodina, Serbia, and one from the Centre for Agricultural Research, Rothamsted, UK). Sirodesmins showing different phytotoxicity on treated cotyledons of cv. Quinta were separated and detected by thin layer chromatography in all analysed isolates (L.m, C-3, St-5 and S-11) except K-113, which neither contained sirodesmin congeners nor did it exhibit activity. By use of high performance liquid chromatography coupled with tandem mass spectrometer, it was possible to identify total of 10 sirodesmins, together with their precursor-phomamide. It was found that the dominant epipolythiodioxopiperazines of the investigated L. maculans isolates were sirodesmin PL, sirodesmin C, and their de-acetylated derivatives., Patogenost fitopatogenih gljiva povezana je sa fitotoksinima, a naročito sa njihovom hemijskom prirodom i količinom. Sirodezmini su fitotoksini iz grupe epipolitiodioksopiperazina, koje proizvodi gljiva Leptosphaeria maculans, uzročnik suve truleži korena i raka stabla uljane repice. Cilj ovog rada bila je detaljna hemijska karakterizacija sirodezmina u pet izolata gljiva (četiri iz Vojvodine i jedan iz Velike Britanije, Centar za poljoprivredna istraživanja, Rothamsted). Kod svih ispitivanih izolata (L. maculans, C-3, St-3, S-11), osim K-113 (koji nije sadržao sirodezmine niti pokazivao aktivnost) tankoslojnom hromatografijom su razdvojeni i detektovani sirodezmini koji su pokazali različitu fitotoksičnost na tretiranim kotiledonima sorte Quinta. Primenom tečne hromatografije visoke efikasnosti, kuplovane sa tandemskim masenim spektrometrom, bilo je moguće identifikovati ukupno 10 sirodezmina, kao i njihov prekursor - fomamid. Utvrđeno je da su dominantni epipolitio-dioksopiperazini ispitivanih izolata L. maculans sirodezmin PL, sirodezmin C i njihovi deacetilovani derivati.",
publisher = "Serbian Chemical Society, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans, Karakterizacija sirodezmina izolovanih iz fitopatogene gljive Leptosphaeria maculans",
pages = "1379-1363",
number = "10",
volume = "77",
doi = "10.2298/JSC111231048M",
url = "conv_21"
}
Mitrović, P., Orčić, D. Z., Sakač, Z., Marjanović-Jeromela, A., Grahovac, N. L., Milošević, D.,& Marisavljević, D.. (2012). Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans. in Journal of the Serbian Chemical Society
Serbian Chemical Society, Belgrade., 77(10), 1363-1379.
https://doi.org/10.2298/JSC111231048M
conv_21
Mitrović P, Orčić DZ, Sakač Z, Marjanović-Jeromela A, Grahovac NL, Milošević D, Marisavljević D. Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans. in Journal of the Serbian Chemical Society. 2012;77(10):1363-1379.
doi:10.2298/JSC111231048M
conv_21 .
Mitrović, Petar, Orčić, Dejan Z., Sakač, Zvonimir, Marjanović-Jeromela, Ana, Grahovac, Nada L., Milošević, Drago, Marisavljević, Dragana, "Characterization of sirodesmins isolated from the phytopathogenic fungus Leptosphaeria maculans" in Journal of the Serbian Chemical Society, 77, no. 10 (2012):1363-1379,
https://doi.org/10.2298/JSC111231048M .,
conv_21 .
