Molecular characterization of PCN populations from Serbia

Abstract

The morphology of potato cyst nematodes (PCN) was until recently almost the only way to identify these quarantine organisms. In the last two decades, molecular analyses contributed to faster and more efficient identification of two Globodera species (Globodera pallida and G. rostochiensis) and allowed insight into the genetic structure of those parts that were practically inaccessible by morphological studies. Molecular characterization was performed in ITS1-5.8S-ITS2 region. The comparison was made with sequences of different foreign PCN populations via NCBI GenBank database. The results of molecular studies showed similarities and differences between local and foreign PCN populations in the part of genome that was studied.

Morfologija cistolikih nematoda krompira (CNK) je do nedavno bila gotovo jedini način identifikacije ovih karantinskih organizama. Molekularne analize su u poslednje dve decenije doprinele bržoj i efikasnijoj identifikaciji dve Globodera vrste (Globodera pallida i G. rostochiensis) i omogućile uvid u genetiku strukturu onih delova koji su praktično bili nedostupni morfološkim studijama. Molekularna karakterizacija je urađena u ITS1-5.8S-ITS2 regionu. Poređenja su vršena sa sekvencama različitih stranih populacija CNK preko NCBI GenBank baze podataka. Rezultati molekularnih studija pokazuju sličnosti i razlike domaćih i stranih populacija CNK u proučavanom delu genoma.