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Pseudomonas syringae - patogen trešnje u Srbiji

dc.creatorGavrilović, Veljko
dc.creatorŽivković, Svetlana
dc.creatorDolovac, Nenad
dc.creatorTrkulja, Nenad
dc.creatorPfaf-Dolovac, Erika
dc.creatorPopović, Tatjana
dc.creatorIvanović, Žarko
dc.date.accessioned2019-04-04T15:57:09Z
dc.date.available2019-04-04T15:57:09Z
dc.date.issued2012
dc.identifier.issn1820-3949
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/224
dc.description.abstractCharacteristics of pathogenic Pseudomonas bacterial strains isolated from cherry in Serbia are presented in the article. Two types of symptoms were observed on cherry trees at few localities with intensive production in Serbia (Belgrade, Čačak, Topola, Šabac, Novi Sad). The first symptom is bud necrosis and the second bacterial canker of cherry branch. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains were levan and HR positive, while negative results were recorded for oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Based on pathogenicity tests and differential GATT tests, investigated strains were divided in two distinct groups: the first group consisted of strains isolated from necrotic cherry branch which caused necrosis on artificially inoculated cherry, pear and lemon fruits, syringae leaves and bean pods, were gelatin and aesculin positive, and tyrosinase and tartrate negative (typical characteristics of P.s. pv. syringae). Contrary, second group strains were isolated from necrotic cherry buds, showed negative results in mentioned pathogenicity tests, gelatin and aesculin tests were negative, while tyrosinase and tartrate were positive (typical characteristics of P.s. pv. morsprunorum). REP PCR analyses showed that strains isolated from necrotic cherry buds belong to P. s pv. morsprunorum compared to referent strain. In contrast, isolates obtained from necrotic cherry branches had unique fingerprint profiles but different from all reference strains. According to the obtained results it was concluded that both pathovars of P. syringae (syringae and morsprunorum) cause necrosis of cherry trees in Serbia.en
dc.description.abstractU radu su prikazane patogene, bakteriološke odlike izolata bakterije Pseudomnas syringae poreklom iz trešnje, kao i njihova molekularna karakterizacija primenom REP-PCR metode. Dva tipa simptoma bolesti zapažena su u savremenim zasadima trešnje na području Beograda, Novog Sada, Topole i Šapca. Prvi tip simptoma se ispoljava u vidu nekroze cvetnih i lisnih pupoljaka trešnje, a drugi u vidu nekroze višegodišnjih grana. Iz nekrotičnog tkiva su izolovane Gram negativne bakterije , koje fluoresciraju na King podlozi B, a glukozu metabolišu isključivo u aerobnim uslovima; bakterija stvara levan i prouzrokuje HR duvana ali ne stvara oksidazu, pektinazu u arginin dehidrolazu (LOPAT +---+) što su odlike karakteristične za bakteriju P. syringae. U pogledu patogenosti izolovanih sojeva, kao i njihovih diferencijalnih biohemijskih testova (GATT) proučavani izolati su svrstani u dve jasno izdiferencirane grupe. Prvu čine sojevi izolovani iz nekrozom zahavćenih grana trešnje, koji prouzrokuju nekrozu inokulisanih plodova trešnje, kruške i limuna, listova jorgovana i mahuna boranije. Ova grupa izolata hidrolizuje želatin i eskulin, ali ne stvaraju tirozinazu i ne metabolišu tartarate. Pomenute patogene i diferencijalne biohemijske odlike su karakteristične za P.s. pv. syringae. Nasuprot njima, sojevi izolovani iz nekrotičnih pupoljaka trešnje negativno reaguju pri pomenutim testovima patogenosti, ne hidrolizuju želatin i eskulin, ali stvaraju tirozinazu i metabolišu tartarate, što su odlike P.s.pv. morsprunorum. REP-PCR analiza takođe ukazuje da sojevi iz nekrotičnih pupoljaka ispoljavaju identične karakteristike kao i referentni soj P.s. pv. morsprunorum. Primenom ovog metoda je takođe utvrđeno da su sojevi izolovani iz obolelih grana trešnje međusobno identični ali da se razlikuju od referentnih sojeva korišćenih u ovim istraživanjima. Na osnovu dobijenih rezultata, tokom ovih istraživanja zaključeno je da oba patogena varijeteta P. syringae patogena voćaka (pv. syrnigae i pv. morsprunorum) parazitiraju trešnju u našim agroekološkim uslovima.sr
dc.publisherInstitute of Pesticides and Environmental Protection
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31018/RS//
dc.rightsopenAccess
dc.sourcePesticidi i fitomedicina
dc.subjectcherriesen
dc.subjectPseudomonas syringaeen
dc.subjectpathogenicityen
dc.subjectGATT testsen
dc.subjectREP PCRen
dc.subjecttrešnjasr
dc.subjectPseudomonas syringaesr
dc.subjectpatogenostsr
dc.subjectGATT testovisr
dc.subjectREP PCRsr
dc.titlePseudomonas syringae: Pathogen of sweet cherry in Serbiaen
dc.titlePseudomonas syringae - patogen trešnje u Srbijisr
dc.typearticle
dc.rights.licenseBY-SA
dc.citation.epage149
dc.citation.issue2
dc.citation.other27(2): 141-149
dc.citation.rankM51
dc.citation.spage141
dc.citation.volume27
dc.type.versionpublishedVersion
dc.identifier.doi10.2298/PIF1202141G
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1381/222.pdf
dc.identifier.rcubconv_326


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