Morphological and breeding characteristics of Cercospora beticola isolates

Abstract

Determination of breeding and morphological characteristics of C. beticola isolates was performed on PDA, MEA i CDA mediums. Determination of morphological characteristics were evaluated by monitoring mycelial growth, texture, color, edge and color of beneath of mycelium. Isolates growth on different temperatures was monitored on PDA medium on 10°C, 15°C, 20°C, 25°C, 30°C, 35°C and 40°C. There was a significant increase in variability of the isolates on PDA, MEA and CDA mediums, but there is a variability in the growth of different isolates of the same medium too. Isolates expressed the variation in color and the width of the edge zone during growth on different substrates. The mycelium texture was cottony on all surfaces, while the beneath was brown to dark-olive colour. At temperatures of 10°C and 40°C isolates of C. beticola did not develop mycelium, and the highest increase was recorded at 25°C.

Utvrđivanje morfoloških i odgajivačkih karakteristika izolata C. beticola izvedeno je na KDA, MEA i CDA podlozi. Utvrđivanje morfoloških karakteristika obuhvatilo je praćenje porasta micelije, teksture, boje, ivične zone i boje naličja micelije. Porast izolata na različitim temperaturama praćen je na KDA podlozi na temperaturama 10°C, 15°C, 20°C, 25°C, 30°C, 35°C i 40°C. Utvrđena je značajna varijabilnost porasta izolata na KDA, MEA i CDA podlozi, a variabilnost postoji i u porastu različitih izolata na istoj podlozi. Izolati su ispoljili i variranje u boji i širini ivične zone tokom porasta na različitim podlogama. Tekstura micelije bila je pamučasta na svim podlogama, dok je boja sa naličja bila mrka do tamno-maslinasta. Na temperaturama 10°C i 40°C izolati C. beticola nisu razvijali miceliju, a najintenzivniji porast je ostvaren na 25°C.