Morphological and cultural characteristics of Monilinia laxa isolates from the stone fruits

Abstract

Morphological and cultural characteristics of Monilinia laxa isolates were studied on PDA and MEA media. The appearance of colonies (color, texture, rosettes, rosettes with black arcs), as well as conidial sze were identified. Growth of mycelium was monitored on both media, while sporulation was followed on PDA medium. Isolates formed aeriform (puffy) mycelium, with significant variations in color on different mediums, but variations in color are present within isolates grown on same medium. On MEA medium isolates are somewhat brighter then isolates grown on PDA medium. All isolates are more or less rosette, but not all of them form black arcs. Conidial size is also characteristic that is variable compared to isolates and medium. Mycelial growth is slightly lower on MEA medium.

Morfološke i odgajivačke karakteristike izolata Monilia laxa proučene su na KDA i MEA hranljivoj podlozi. Determinisani su izgled kolonija (boja, tekstura, rozetavost, formiranje koncentričnih prstenova), kao i dimenzije konidija. Od odgajivačkih karakteristika proučen je porast micelije i sporulacija. Porast micelije je praćen na obe hranljive podloge, a sporulacija samo na podlozi KDA. Svi izolati formiraju vazdušastu miceliju, sa značajnim variranjem u boji na različitim podlogama, ali je variranje u boji prisutno i između izolata gajenih na istoj podlozi. Na MEA podlozi izolati su nešto svetliji u odnosu na izolate sa KDA podloge. Svi izolati su više ili manje intenzivno rozetavi, ali ne formiraju svi prstenove. Veličina konidija je takođe karakteristika koja varira u odnosu na izolat i podlogu. Porast micelije je nešto manji na MEA podlozi.