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Identifikacija Colletotrichum acutatum sa ploda nektarine

dc.creatorŽivković, Svetlana
dc.creatorGavrilović, Veljko
dc.creatorOro, Violeta
dc.creatorPfaf-Dolovac, Erika
dc.creatorStošić, Stefan
dc.creatorKuzmanović, Slobodan
dc.creatorIvanović, Žarko
dc.date.accessioned2019-04-04T15:57:50Z
dc.date.available2019-04-04T15:57:50Z
dc.date.issued2012
dc.identifier.issn0372-7866
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/246
dc.description.abstractIsolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum.en
dc.description.abstractIzolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.sr
dc.publisherInstitute for Plant Protection and Environment, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31018/RS//
dc.rightsopenAccess
dc.sourceZaštita bilja
dc.subjectanthracnoseen
dc.subjectnectarineen
dc.subjectColletotrichum acutatumen
dc.subjectidentificationen
dc.subjectantraknozasr
dc.subjectnektarinasr
dc.subjectColletotrichum acutatumsr
dc.subjectidentifikacijasr
dc.titleIdentification of Colletotrichum acutatum from nectarine fruiten
dc.titleIdentifikacija Colletotrichum acutatum sa ploda nektarinesr
dc.typearticle
dc.rights.licenseARR
dc.citation.epage138
dc.citation.issue3
dc.citation.other63(3): 130-138
dc.citation.rankM51
dc.citation.spage130
dc.citation.volume63
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1402/244.pdf
dc.identifier.rcubconv_246


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