Antifungal activity of indigenous rhizospheric isolates Pseudomonas spp. against Venturia inaequalis

Abstract

Plant diseases caused by phytopathogenic fungi are one of the major problems in orchard plants in Serbia. To avoid the use of chemical agents in high percent, biological control is becoming promising method in plant disease management. It has been showed that apple is host of Venturia inaequalis, a very aggressive phytopathogenic fungus known for production scab. In search for ecological ways for prevention and reduction of disease symptoms, biological control with indigenous fluorescent Pseudomonas isolates has been offered as a possible solution. The aim of this study was to examine antifungal activity of different indigenous fluorescent Pseudomonas isolates (Q16, B25 and PS2) against phytopathogenic fungus V. inaequalis which had infected apple. Indigenous Pseudomonas isolates showed in vitro inhibition of fungal growth 13.85% to 54.36 % on WA and 11.37 % to 58.33% on PDA. These isolates should be further analyzed in order to classify them as promising group of biocontrol agents against diseases of apple scab caused by V. inaequalis.

Biljne bolesti prouzrokovane fitopatogenim gljivicama su jedan od glavnih problema u voćnjacima u Srbiji. Biološka kontrola patogena sve više se koristi u upravljanju biljnim bolestima kako bi se izbeglo korišćenje hemijskih agenasa u velikoj meri. Jabuka kao veoma značajna voćarska kultura je domaćin veoma agresivne fitopatogene gljive Venuria inaequalis, prouzrokovača čađave krastavosti ploda. U potrazi za ekološkim načinima za sprečavanje pojave i smanjenje simptoma bolesti, biološka kontrola autohtonim izolatima iz roda Pseudomonas je potencijalno moguće rešenje. Cilj ovog istraživanja bio je da se ispita antifungalna aktivnost različitih autohtonih izolata Pseudomonas (P16, B25 i PS2) protiv fitopatogene gljive V.inaequalis koja zaražava jabuku. Autohtoni Pseudomonas izolati su u in vitro uslovima pokazali inhibiciju rasta kolonija gljivice od 13.85% do 54.36% na WA podlozi i od 11.37% do 58.33% na PDA. Ove izolate treba dalje analizirati kao grupu bioloških agenasa koja može imati veliki potencijal u suzbijanju V.inaequalis, prouzrokovača čađave krastavosti jabuke.