Show simple item record

Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji

dc.creatorGavrilović, Veljko
dc.creatorIvanović, Žarko
dc.creatorPopović, Tatjana
dc.creatorŽivković, Svetlana
dc.creatorStanković, Slaviša
dc.creatorBerić, Tanja
dc.creatorFira, Đorđe
dc.date.accessioned2019-04-04T15:57:58Z
dc.date.available2019-04-04T15:57:58Z
dc.date.issued2013
dc.identifier.issn0534-0012
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/251
dc.description.abstractDuring past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia.en
dc.description.abstractTokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.sr
dc.publisherSerbian Genetics Society
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31018/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173026/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceGenetika-Belgrade
dc.subjectbud necrosisen
dc.subjectcherryen
dc.subjectGATTen
dc.subjectpathogenicityen
dc.subjectP. syringae pv. morsprunorumen
dc.subjectplumen
dc.subjectREP-PCRen
dc.titleGenetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbiaen
dc.titleGenetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbijisr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage961
dc.citation.issue3
dc.citation.other45(3): 953-961
dc.citation.rankM23
dc.citation.spage953
dc.citation.volume45
dc.type.versionpublishedVersion
dc.identifier.doi10.2298/GENSR1303953G
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1407/249.pdf
dc.identifier.rcubconv_304
dc.identifier.scopus2-s2.0-84894565838
dc.identifier.wos000330186600030


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record