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Selekcija i RAPD analiza izolata Pseudomonas spp. koji poboljšavaju biološku sposobnost krtola krompira

dc.creatorPoštić, Dobrivoj
dc.creatorStarović, Mira
dc.creatorPopović, Tatjana
dc.creatorBosnić, Predrag
dc.creatorStanojković-Sebić, Aleksandra
dc.creatorPivić, Radmila
dc.creatorJošić, Dragana
dc.date.accessioned2019-04-04T15:58:04Z
dc.date.available2019-04-04T15:58:04Z
dc.date.issued2013
dc.identifier.issn0534-0012
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/253
dc.description.abstractIndoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 µ gmL- 1 after 24h of cultivation and 4.08 to 26.4 µ gmL-1 after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20°C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability.en
dc.description.abstractIzolati Pseudomonas iz rizosfere kukuruza (Q4 i Q20), lucerke (Q1 i Q16) i divlje bele deteline (B25) selektovani su na osnovu produkcije indolsirćetne kiseline (IAA) radi ispitivanja efekta na biološku sposobnosti semenskih krtola krompira. Produkcija IAA iznosila je 4.09 do 15.9 µ gmL-1 posle 24h i 4.08 do 26.4 µ gmL-1 posle 48h kultivacije. Upoređivanje izolata izvršeno je na osnovu RAPD analize. Na osnovu RAPD profila selektovanih Pseudomonas spp. izolata, dobijenih amplifikacijom BC318, AF14, SPH1 i AP10 prajmerima, ustanovljen je visok stepen različitosti između 4 izolata (44 do 68%), što je potvrdilo efikasnost RAPD metode pri upoređivanju izolata. Efekat na biološku sposobnosti semenskih krtola krompira (industrijski važnu sortu Pirol) ispitivan je tokom sedam nedelja naklijavanja na temperaturi 18-20°C. Krtole tretirane selektovanim izolatima bakterija formirale su nešto manji broj klica, ali je dužina klica statistički značajno veća i do 129,9% u odnosu na kontrolu. Ostvaren je kapacitet klijanja veći do 64,6% u odnosu na kontrolu. Dobijeni rezultati ukazuju na pozitivan efekat selektovanih Pseudomonas izolata koji produkuju IAA na dužinu klica i kapacitet klijanja kao parametara koji određuju biološku sposobnosti semenskih krtola krompira.sr
dc.publisherSerbian Genetics Society
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46007/RS//
dc.rightsopenAccess
dc.sourceGenetika-Belgrade
dc.subjectPseudomonas spp.en
dc.subjectIAAen
dc.subjectRAPDen
dc.subjectbiological viabilityen
dc.subjectpotatoen
dc.subjecttuberen
dc.subjectsprouten
dc.subjectsprouting capacityen
dc.titleSelection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubersen
dc.titleSelekcija i RAPD analiza izolata Pseudomonas spp. koji poboljšavaju biološku sposobnost krtola krompirasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage249
dc.citation.issue1
dc.citation.other45(1): 237-249
dc.citation.rankM23
dc.citation.spage237
dc.citation.volume45
dc.type.versionpublishedVersion
dc.identifier.doi10.2298/GENSR1301237P
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1409/251.pdf
dc.identifier.rcubconv_303
dc.identifier.scopus2-s2.0-84880578817
dc.identifier.wos000320985900023


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