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Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli

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2013
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Authors
Popović, Tatjana
Jošić, Dragana
Starović, Mira
Milovanović, Predrag
Dolovac, Nenad
Poštić, Dobrivoj
Stanković, Slaviša
article (publishedVersion)
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Abstract
Thirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35°C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity l...evel to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.

Keywords:
Xanthomonas campestris / phenotypic and genotypic characterization
Source:
Archives of Biological Sciences, 2013, 65, 2, 585-593
Publisher:
  • University of Belgrade, University of Novi Sad
Funding / projects:
  • info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46007/RS// (RS-46007)
  • info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46007/RS// (RS-46007)

DOI: 10.2298/ABS1302585P

ISSN: 0354-4664

WoS: 000318826000022

Scopus: 2-s2.0-84876956894
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URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/279
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