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dc.creatorPopović, Tatjana
dc.creatorJošić, Dragana
dc.creatorStarović, Mira
dc.creatorMilovanović, Predrag
dc.creatorDolovac, Nenad
dc.creatorPoštić, Dobrivoj
dc.creatorStanković, Slaviša
dc.date.accessioned2019-04-04T15:58:52Z
dc.date.available2019-04-04T15:58:52Z
dc.date.issued2013
dc.identifier.issn0354-4664
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/279
dc.description.abstractThirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35°C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity level to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.en
dc.publisherUniversity of Belgrade, University of Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46007/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46007/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceArchives of Biological Sciences
dc.subjectXanthomonas campestrisen
dc.subjectphenotypic and genotypic characterizationen
dc.titlePhenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccolien
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage593
dc.citation.issue2
dc.citation.other65(2): 585-593
dc.citation.rankM23
dc.citation.spage585
dc.citation.volume65
dc.type.versionpublishedVersion
dc.identifier.doi10.2298/ABS1302585P
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1434/277.pdf
dc.identifier.rcubconv_76
dc.identifier.scopus2-s2.0-84876956894
dc.identifier.wos000318826000022


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