Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics
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2013
Authors
Mitrovski-Bogdanović, Ana S
Petrović, Anđeljko

Mitrović, Milana

Ivanović, Ana

Zikić, Vladimir
Stary, Petr
Vorburger, Christoph

Tomanović, Željko
Article (Published version)

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The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp.... and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium.
Keywords:
aphid parasitoids / host specialization / Praon sambuci sp.n. / Praon longicaudus sp.n. / Longicaudus trirhodus / Aphis sambuciSource:
Annals of the Entomological Society of America, 2013, 106, 2, 170-180Publisher:
- Oxford Univ Press Inc, Cary
Funding / projects:
- SCOPES program of the Swiss National Science Foundation - IZ73Z0_128174
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-43001)
- Entomology Institute project (Academy of Sciences of the Czech Republic) - AV0Z50070508
DOI: 10.1603/AN12100
ISSN: 0013-8746
WoS: 000316299600004
Scopus: 2-s2.0-84879634178
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IZBISTY - JOUR AU - Mitrovski-Bogdanović, Ana S AU - Petrović, Anđeljko AU - Mitrović, Milana AU - Ivanović, Ana AU - Zikić, Vladimir AU - Stary, Petr AU - Vorburger, Christoph AU - Tomanović, Željko PY - 2013 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/289 AB - The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp. and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium. PB - Oxford Univ Press Inc, Cary T2 - Annals of the Entomological Society of America T1 - Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics EP - 180 IS - 2 SP - 170 VL - 106 DO - 10.1603/AN12100 ER -
@article{ author = "Mitrovski-Bogdanović, Ana S and Petrović, Anđeljko and Mitrović, Milana and Ivanović, Ana and Zikić, Vladimir and Stary, Petr and Vorburger, Christoph and Tomanović, Željko", year = "2013", abstract = "The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp. and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium.", publisher = "Oxford Univ Press Inc, Cary", journal = "Annals of the Entomological Society of America", title = "Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics", pages = "180-170", number = "2", volume = "106", doi = "10.1603/AN12100" }
Mitrovski-Bogdanović, A. S., Petrović, A., Mitrović, M., Ivanović, A., Zikić, V., Stary, P., Vorburger, C.,& Tomanović, Ž.. (2013). Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics. in Annals of the Entomological Society of America Oxford Univ Press Inc, Cary., 106(2), 170-180. https://doi.org/10.1603/AN12100
Mitrovski-Bogdanović AS, Petrović A, Mitrović M, Ivanović A, Zikić V, Stary P, Vorburger C, Tomanović Ž. Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics. in Annals of the Entomological Society of America. 2013;106(2):170-180. doi:10.1603/AN12100 .
Mitrovski-Bogdanović, Ana S, Petrović, Anđeljko, Mitrović, Milana, Ivanović, Ana, Zikić, Vladimir, Stary, Petr, Vorburger, Christoph, Tomanović, Željko, "Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics" in Annals of the Entomological Society of America, 106, no. 2 (2013):170-180, https://doi.org/10.1603/AN12100 . .