PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes
Abstract
Several populations of the stem-mining weevil Mecinus janthinus Germar species complex (Mecinini, Curculionidae), identified based on morphological characteristics, have been introduced in North America for the biological control of invasive toadflaxes of European origin: Linaria vulgaris Miller and L. dalmatica (L.) Miller (Plantaginaceae). According to the mitochondrial cytochrome oxidase subunit II (COII) gene haplotype divergence of Mecinus janthinus species complex, a total of 20 M. janthinus s.s., 3 M. janthinus s.l. of the 'speciosa' genotype and 29 M. janthiniformis haplotypes have been recorded across their native range in central and southeastern Europe. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) diagnostic assay of COII gene using Hpy188III and MnlI enzyme-mix, was developed for fast and cost-effective discrimination of these morphologically very similar cryptic weevil species. It is shown that digestion generates unique 4-fra...gment restriction profile in M. janthinus s.s., 2-fragment profile in M. janthiniformis and 3-fragment profile in M. janthinus s.l. 'speciosa' group of haplotypes, allowing precise identification of each species or genotype. The proposed method represents a practical tool for fast and accurate identification of the target biocontrol agents and should prevent using inappropriate weevil species in redistribution programs for biological control of invasive toadflax species.
Keywords:
Curculionidae / Invasive toadflaxes / Mecinus janthiniformis / Mecinus janthinus / Molecular identification of biocontrol agent / PCR-RFLP diagnostic assaySource:
BioControl, 2013, 58, 4, 563-573Publisher:
- Springer, Dordrecht
Funding / projects:
- Ministry of Forests and Range, British Columbia Provincial Government
- Wyoming Biological Control Steering Committee, USDA-APHIS-CPHST
- USDA Forest Service and the Montana Noxious Weed Trust Fund through the Montana State University
- California Department of Food and Agriculture
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-43001)
DOI: 10.1007/s10526-013-9506-2
ISSN: 1386-6141
WoS: 000323908400011
Scopus: 2-s2.0-84880508519
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Institution/Community
IZBISTY - JOUR AU - Toševski, Ivo AU - Jović, Jelena AU - Krstić, Oliver AU - Gassmann, Andre PY - 2013 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/295 AB - Several populations of the stem-mining weevil Mecinus janthinus Germar species complex (Mecinini, Curculionidae), identified based on morphological characteristics, have been introduced in North America for the biological control of invasive toadflaxes of European origin: Linaria vulgaris Miller and L. dalmatica (L.) Miller (Plantaginaceae). According to the mitochondrial cytochrome oxidase subunit II (COII) gene haplotype divergence of Mecinus janthinus species complex, a total of 20 M. janthinus s.s., 3 M. janthinus s.l. of the 'speciosa' genotype and 29 M. janthiniformis haplotypes have been recorded across their native range in central and southeastern Europe. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) diagnostic assay of COII gene using Hpy188III and MnlI enzyme-mix, was developed for fast and cost-effective discrimination of these morphologically very similar cryptic weevil species. It is shown that digestion generates unique 4-fragment restriction profile in M. janthinus s.s., 2-fragment profile in M. janthiniformis and 3-fragment profile in M. janthinus s.l. 'speciosa' group of haplotypes, allowing precise identification of each species or genotype. The proposed method represents a practical tool for fast and accurate identification of the target biocontrol agents and should prevent using inappropriate weevil species in redistribution programs for biological control of invasive toadflax species. PB - Springer, Dordrecht T2 - BioControl T1 - PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes EP - 573 IS - 4 SP - 563 VL - 58 DO - 10.1007/s10526-013-9506-2 ER -
@article{ author = "Toševski, Ivo and Jović, Jelena and Krstić, Oliver and Gassmann, Andre", year = "2013", abstract = "Several populations of the stem-mining weevil Mecinus janthinus Germar species complex (Mecinini, Curculionidae), identified based on morphological characteristics, have been introduced in North America for the biological control of invasive toadflaxes of European origin: Linaria vulgaris Miller and L. dalmatica (L.) Miller (Plantaginaceae). According to the mitochondrial cytochrome oxidase subunit II (COII) gene haplotype divergence of Mecinus janthinus species complex, a total of 20 M. janthinus s.s., 3 M. janthinus s.l. of the 'speciosa' genotype and 29 M. janthiniformis haplotypes have been recorded across their native range in central and southeastern Europe. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) diagnostic assay of COII gene using Hpy188III and MnlI enzyme-mix, was developed for fast and cost-effective discrimination of these morphologically very similar cryptic weevil species. It is shown that digestion generates unique 4-fragment restriction profile in M. janthinus s.s., 2-fragment profile in M. janthiniformis and 3-fragment profile in M. janthinus s.l. 'speciosa' group of haplotypes, allowing precise identification of each species or genotype. The proposed method represents a practical tool for fast and accurate identification of the target biocontrol agents and should prevent using inappropriate weevil species in redistribution programs for biological control of invasive toadflax species.", publisher = "Springer, Dordrecht", journal = "BioControl", title = "PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes", pages = "573-563", number = "4", volume = "58", doi = "10.1007/s10526-013-9506-2" }
Toševski, I., Jović, J., Krstić, O.,& Gassmann, A.. (2013). PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes. in BioControl Springer, Dordrecht., 58(4), 563-573. https://doi.org/10.1007/s10526-013-9506-2
Toševski I, Jović J, Krstić O, Gassmann A. PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes. in BioControl. 2013;58(4):563-573. doi:10.1007/s10526-013-9506-2 .
Toševski, Ivo, Jović, Jelena, Krstić, Oliver, Gassmann, Andre, "PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes" in BioControl, 58, no. 4 (2013):563-573, https://doi.org/10.1007/s10526-013-9506-2 . .