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dc.creatorKuzmanović, Nemanja
dc.creatorIvanović, Milan M
dc.creatorProkić, Anđelka
dc.creatorGašić, Katarina
dc.creatorBlagojević, Nevena
dc.creatorPulawska, Joanna
dc.creatorObradović, Aleksa
dc.date.accessioned2019-04-04T15:59:30Z
dc.date.available2019-04-04T15:59:30Z
dc.date.issued2013
dc.identifier.issn0929-1873
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/299
dc.description.abstractCrown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.en
dc.publisherSpringer, Dordrecht
dc.relationMinistry of Education, Science and Technological Development, Republic of Serbia
dc.relationCOST Action - 873
dc.relationPolish Ministry of Science and Higher Education - 118/N-COST/2008/0
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46008/RS//
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Plant Pathology
dc.subjectCrown gallen
dc.subjectApricoten
dc.subjectTumorigenicen
dc.subjectPathogenic plasmiden
dc.subjectOpinesen
dc.titleIdentification and characterization of Agrobacterium spp. isolated from apricot in Serbiaen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage16
dc.citation.issue1
dc.citation.other137(1): 11-16
dc.citation.rankM21
dc.citation.spage11
dc.citation.volume137
dc.type.versionpublishedVersion
dc.identifier.doi10.1007/s10658-013-0229-0
dc.identifier.rcubconv_858
dc.identifier.scopus2-s2.0-84881230679
dc.identifier.wos000322880200003


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