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Fusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbiji

dc.creatorRistić, Danijela
dc.creatorPavlović, Snežana
dc.creatorTrkulja, Nenad
dc.creatorPfaf-Dolovac, Erika
dc.creatorDolovac, Nenad
dc.creatorStarović, Mira
dc.date.accessioned2019-04-04T15:59:34Z
dc.date.available2019-04-04T15:59:34Z
dc.date.issued2014
dc.identifier.issn0372-7866
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/302
dc.description.abstractDuring 2013, 14 samples of infected seed grown calendula were collected from two commercially grown crops in the localities in Pančevo and Plandište and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a significant seed infection ranging from 2-8%, with phytopathogenic fungi of the genus Fusarium, was found. From the infected calendula seed, monosporial cultures, based on morphology, were identified as Fusarium verticillioides and Fusarium cf. incarnatum, and their pathogenicity proved on artificially inoculated calendula seedlings. Molecular detection was performed by PCR and amplification of the TEF-1α protein gene. Further studies will include molecular identification of this isolate to the species level, a definitive taxonomic determination of the Serbian isolate and a comparison with other isolates and species of Fusarium genus infecting calendula worldwide.en
dc.description.abstractTokom 2013. godine, na dva lokaliteta komercijalne proizvodnje nevena u Pančevu i Plandištu, prikupljeno je 14 uzoraka zaraženog semena gajenog nevena i analizirano na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i značajan stepen zaraze fitopatogenim gljivama iz roda Fusarium, 2-8%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima nevena, a na osnovu morfoloških svojstava identifikovane su kao Fusarium verticillioides i Fusarium cf. incarnatum. Molekularna detekcija obavljena je primenom PCR i amplifikacije proteinskog gena TEF-1α. Dalja istraživanja obuhvatiće primenu molekularne identifikacije, do nivoa vrste uz određivanje tačnog taksonomskog mesta izolata iz Srbije patogenih za neven poređenjem sa drugim izolatima i vrstama roda Fusarium spp. u svetu.sr
dc.publisherInstitute for Plant Protection and Environment, Belgrade
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31018/RS//
dc.rightsopenAccess
dc.sourceZaštita bilja
dc.subjectFusarium spp.en
dc.subjectmolecular detectionen
dc.subjectmorphological propertiesen
dc.subjectpathogenicity testen
dc.subjectcalendulaen
dc.subjectFusarium spp.sr
dc.subjectmolekularna detekcijasr
dc.subjectmorfološke osobinesr
dc.subjecttest patogenostisr
dc.subjectnevensr
dc.titleFusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbiaen
dc.titleFusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbijisr
dc.typearticle
dc.rights.licenseARR
dc.citation.epage116
dc.citation.issue3
dc.citation.other65(3): 111-116
dc.citation.rankM52
dc.citation.spage111
dc.citation.volume65
dc.type.versionpublishedVersion
dc.identifier.doi10.5937/zasbilj1403111R
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1448/300.pdf
dc.identifier.rcubconv_278


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