Characterization and phylogenetic diversity of Agrobacterium vitis from Serbia based on sequence analysis of 16S-23S rRNA internal transcribed spacer (ITS) region
Authorized Users Only
2014
Authors
Kuzmanović, Nemanja
Ivanović, Milan
Prokić, Anđelka
Gašić, Katarina

Zlatković, Nevena
Obradović, Aleksa

Article (Published version)

Metadata
Show full item recordAbstract
Serious outbreaks of grapevine crown gall disease were observed in major Serbian viticultural regions during the last five years. Tumorigenic Agrobacterium vitis was identified as a causal agent by using conventional bacteriological and molecular tests. The 36 studied strains of A. vitis showed homogeneous biochemical and physiological characteristics, but were heterogeneous in their pathogenic properties, especially on tomato and sunflower. Furthermore, genetic differences related to chromosomal and plasmid DNA were observed. The Ti plasmid of 35 strains was classified as the octopine/cucumopine (O/C) type, whereas one was classified as the vitopine (V) type. The O/C strains were further divided into O/C-1 and O/C-2 groups based on PCR analysis. Moreover, the sequence analysis of the 16S-23S rRNA ITS region provided robust and precise delineation of studied strains. Although a high level of genetic diversity in A. vitis strains from Serbia was revealed by using this approach, their ge...notypic relatedness with the strains from other countries suggested their common origin. Also, association between the chromosomal and plasmid DNA was determined for some phylogenetic groups and clusters.
Keywords:
Crown gall / Grapevine / 16S-23SrRNAITS region / Ti plasmid / Opine typeSource:
European Journal of Plant Pathology, 2014, 140, 4, 757-768Publisher:
- Springer, Dordrecht
Projects:
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-46008)
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-316004)
DOI: 10.1007/s10658-014-0507-5
ISSN: 0929-1873