In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
Keywords:
bacteriophages / host range / fire blight / biological control
Source:
XIII International Workshop on Fire Blight, 2014, 1056, 123-126
TY - CONF
AU - Gašić, Katarina
AU - Ivanović, M.
AU - Kuzmanović, Nemanja
AU - Prokić, Anđelka
AU - Obradović, Aleksa
AU - Šević, Milan
PY - 2014
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/311
AB - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
PB - Int Soc Horticultural Science, Leuven 1
C3 - XIII International Workshop on Fire Blight
T1 - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
EP - 126
SP - 123
VL - 1056
DO - 10.17660/ActaHortic.2014.1056.17
ER -
@conference{
author = "Gašić, Katarina and Ivanović, M. and Kuzmanović, Nemanja and Prokić, Anđelka and Obradović, Aleksa and Šević, Milan",
year = "2014",
abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "XIII International Workshop on Fire Blight",
title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential",
pages = "126-123",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.17"
}
Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in XIII International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 1056, 123-126.
https://doi.org/10.17660/ActaHortic.2014.1056.17
Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in XIII International Workshop on Fire Blight. 2014;1056:123-126.
doi:10.17660/ActaHortic.2014.1056.17 .
Gašić, Katarina, Ivanović, M., Kuzmanović, Nemanja, Prokić, Anđelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in XIII International Workshop on Fire Blight, 1056 (2014):123-126,
https://doi.org/10.17660/ActaHortic.2014.1056.17 . .
