Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method
Apstrakt
Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper. In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia. First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots. Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits ('Williams'). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose. After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains.
Ključne reči:
quince / pathogenicity / biochemical characteristics / REP-PCRIzvor:
XIII International Workshop on Fire Blight, 2014, 1056, 169-172Izdavač:
- Int Soc Horticultural Science, Leuven 1
DOI: 10.17660/ActaHortic.2014.1056.26
ISSN: 0567-7572
WoS: 000357733400026
Scopus: 2-s2.0-84912076131
Institucija/grupa
IZBISTY - CONF AU - Gavrilović, Veljko AU - Ivanović, Žarko AU - Popović, Tatjana AU - Živković, Svetlana PY - 2014 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/320 AB - Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper. In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia. First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots. Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits ('Williams'). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose. After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains. PB - Int Soc Horticultural Science, Leuven 1 C3 - XIII International Workshop on Fire Blight T1 - Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method EP - 172 SP - 169 VL - 1056 DO - 10.17660/ActaHortic.2014.1056.26 ER -
@conference{ author = "Gavrilović, Veljko and Ivanović, Žarko and Popović, Tatjana and Živković, Svetlana", year = "2014", abstract = "Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper. In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia. First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots. Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits ('Williams'). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose. After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains.", publisher = "Int Soc Horticultural Science, Leuven 1", journal = "XIII International Workshop on Fire Blight", title = "Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method", pages = "172-169", volume = "1056", doi = "10.17660/ActaHortic.2014.1056.26" }
Gavrilović, V., Ivanović, Ž., Popović, T.,& Živković, S.. (2014). Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method. in XIII International Workshop on Fire Blight Int Soc Horticultural Science, Leuven 1., 1056, 169-172. https://doi.org/10.17660/ActaHortic.2014.1056.26
Gavrilović V, Ivanović Ž, Popović T, Živković S. Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method. in XIII International Workshop on Fire Blight. 2014;1056:169-172. doi:10.17660/ActaHortic.2014.1056.26 .
Gavrilović, Veljko, Ivanović, Žarko, Popović, Tatjana, Živković, Svetlana, "Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method" in XIII International Workshop on Fire Blight, 1056 (2014):169-172, https://doi.org/10.17660/ActaHortic.2014.1056.26 . .