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dc.creatorPopović, Tatjana
dc.creatorBalaž, Jelica
dc.creatorStanković, Slaviša
dc.date.accessioned2019-04-04T16:00:53Z
dc.date.available2019-04-04T16:00:53Z
dc.date.issued2014
dc.identifier.issn0354-4664
dc.identifier.urihttps://plantarum.izbis.bg.ac.rs/handle/123456789/345
dc.description.abstractA diagnostic method based on nested-PCR, followed by ELISA and conventional bacteriology tests, for the rapid and reliable detection of halo blight pathogen Pseudomonas savastanoi pv. phaseolicola (Psp) collected from infected bean leaves and seeds is described. Psp formed white, small and flat colonies on nutrient agar medium, creamy white, flat and circular on Milk-Tween agar medium and light yellow, convex and shiny on modified sucrose peptone agar medium. Eighteen Gram-negative, catalase-positive and oxidase-negative strains were subjected to nested PCR with primers P 5.1/P 3.1 and P 5.2/P 3.2, which directed the amplification of the 450 bp target DNA fragment in all tested strains. According to the results of DAS-and PTA-ELISA with respect to reactivity to specific antibodies, all analyzed strains belonged to Psp bacterium. Pathogenicity was tested on bean pods and cotyledon leaves, on which greasy spots were formed. Psp did not cause hypersensitive reaction on the leaves of tobacco and geranium. Strains produced levan, fluorescent pigment, oxidative metabolism of glucose, did not reduce nitrate, did not produce indole and H2S, did not hydrolyze starch, gelatin and esculin; they produced acid from glucose, mannose, sucrose and glycerol, and did not produce acid from maltose, starch, esculin, dulcite, sorbitol, inositol and erythritol.en
dc.publisherUniversity of Belgrade, University of Novi Sad
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceArchives of Biological Sciences
dc.subjectPseudomonas savastanoi pv. Phaseolicolaen
dc.subjecthalo blighten
dc.subjectbeanen
dc.subjectidentificationen
dc.titleA method for the rapid detection and identification of halo blight pathogen on common beanen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage1399
dc.citation.issue4
dc.citation.other66(4): 1393-1399
dc.citation.rankM23
dc.citation.spage1393
dc.citation.volume66
dc.description.otherThis article has been retracted. Link to the retraction: [http://dx.doi.org/10.2298/ABS150609074E]
dc.type.versionpublishedVersion
dc.identifier.doi10.2298/ABS1404393P
dc.identifier.fulltexthttps://plantarum.izbis.bg.ac.rs//bitstream/id/1482/343.pdf
dc.identifier.scopus2-s2.0-84921687597
dc.identifier.wos000346477900013


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