Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in serbia
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Authors
Popović, TatjanaBalaž, Jelica
Ignjatov, Maja
Mitrović, Petar
Gavrilović, Veljko
Jošić, Dragana
Article (Published version)
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During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.
Keywords:
black rot / oilseed rape / ERIC / (GTG)(5) / 16S rDNASource:
Journal of Plant Pathology, 2014, 96, 3, 553-560Publisher:
- Edizioni Ets, Pisa
Projects:
- Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-43010)
- New indigenous bacterial isolates Lysobacter and Pseudomonas as an important source of metabolites useful for biotechnology, plant growth stimulation and disease control: from isolates to inoculants (RS-46007)
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