Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in serbia

Popović, Tatjana; Balaž, Jelica; Ignjatov, Maja; Mitrović, Petar; Gavrilović, Veljko; Jošić, Dragana

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Popović, Tatjana
Balaž, Jelica
Ignjatov, Maja

Mitrović, Petar
Gavrilović, Veljko
Jošić, Dragana

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Abstract

During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.

Keywords:

black rot / oilseed rape / ERIC / (GTG)(5) / 16S rDNA

Source:
Journal of Plant Pathology, 2014, 96, 3, 553-560

Publisher:

Edizioni Ets, Pisa

Projects:

ISSN: 1125-4653

WoS: 000348437300013

Scopus: 2-s2.0-84920067135

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TY  - JOUR
AU  - Popović, Tatjana
AU  - Balaž, Jelica
AU  - Ignjatov, Maja
AU  - Mitrović, Petar
AU  - Gavrilović, Veljko
AU  - Jošić, Dragana
PY  - 2014
UR  - http://plantarum.izbis.bg.ac.rs/handle/123456789/366
AB  - During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.
PB  - Edizioni Ets, Pisa
T2  - Journal of Plant Pathology
T1  - Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in serbia
EP  - 560
IS  - 3
SP  - 553
VL  - 96
ER  -

@article{
author = "Popović, Tatjana and Balaž, Jelica and Ignjatov, Maja and Mitrović, Petar and Gavrilović, Veljko and Jošić, Dragana",
year = "2014",
url = "http://plantarum.izbis.bg.ac.rs/handle/123456789/366",
abstract = "During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.",
publisher = "Edizioni Ets, Pisa",
journal = "Journal of Plant Pathology",
title = "Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in serbia",
pages = "560-553",
number = "3",
volume = "96"
}

Popović T, Balaž J, Ignjatov M, Mitrović P, Gavrilović V, Jošić D. Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in serbia. Journal of Plant Pathology. 2014;96(3):553-560

Popović, T., Balaž, J., Ignjatov, M., Mitrović, P., Gavrilović, V.,& Jošić, D. (2014). Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in serbia.
Journal of Plant PathologyEdizioni Ets, Pisa., 96(3), 553-560.

Popović Tatjana, Balaž Jelica, Ignjatov Maja, Mitrović Petar, Gavrilović Veljko, Jošić Dragana, "Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in serbia" 96, no. 3 (2014):553-560