The symptoms pf pear decline are present in Serbia since 1970s. Thirty years later it was proved that the causal agent of this decline is a phytoplasma. This parasite is widespread in Europe and the USA as well as the countries of former Yugoslavia like Bosna and Hercegovina and Croatia. The presence of the phytoplasmas was confirmed using electron microscopy and the molecular analysis if the pear samples from a number of localities in Serbia between 2004-2007. The phytoplasma was observed on the ultrathin sections of the main leaf vascular bundle from the diseased pear tree. The shape, structure and size of 350-480 x 600-700nm, are typical ones for the phytoplasmas. PCR analysis using the universal primer R16F2n/R16R2 as well as the specific primer fO1/rO1 also confirmed phytoplasmas as the causal agent of pear decline.
Simptomi propadanja kruške su prisutni u Srbiji od sedamdesetih godina prošlog veka. Mnogo godina kasnije, dokazano je da je prouzrokovač ovog propadanja fitoplazma kruške (PD). Ova pojava vrlo je raširena u Evropi i u Severnoj Americi, prisutna je i u susednim zemljama bivše Jugoslavije, Bosni i Hercegovini i Hrvatskoj. Prisustvo ove fitoplazme utvrđivano je primenom elektronske mikroskopije i molekularnom analizom uzoraka poreklom iz više lokaliteta u Srbiji, u periodu od 2004-2007 godine. Na ultratankim presecima floemskog tkiva glavnih lisnih nerava obolelih stabala kruške, utvrđeno je prisustvo organizama, koji po obliku, veličini (350-480 x 600-700nm) i strukturi, odgovaraju fitoplazmama. Rezultati PCR analiza, sa univerzalnim R16F2n/R16R2 i specifičnim prajmerom fO1/rO1 ukazali su na prisustvo fitoplazme Pear decline.
TY - JOUR
AU - Starović, Mira
AU - Ivanović, Žarko
AU - Aleksić, Goran
AU - Kuzmanović, Slobodan
AU - Stojanović, Saša
AU - Živković, Svetlana
AU - Gavrilović, Veljko
PY - 2006
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/37
AB - The symptoms pf pear decline are present in Serbia since 1970s. Thirty years later it was proved that the causal agent of this decline is a phytoplasma. This parasite is widespread in Europe and the USA as well as the countries of former Yugoslavia like Bosna and Hercegovina and Croatia. The presence of the phytoplasmas was confirmed using electron microscopy and the molecular analysis if the pear samples from a number of localities in Serbia between 2004-2007. The phytoplasma was observed on the ultrathin sections of the main leaf vascular bundle from the diseased pear tree. The shape, structure and size of 350-480 x 600-700nm, are typical ones for the phytoplasmas. PCR analysis using the universal primer R16F2n/R16R2 as well as the specific primer fO1/rO1 also confirmed phytoplasmas as the causal agent of pear decline.
AB - Simptomi propadanja kruške su prisutni u Srbiji od sedamdesetih godina prošlog veka. Mnogo godina kasnije, dokazano je da je prouzrokovač ovog propadanja fitoplazma kruške (PD). Ova pojava vrlo je raširena u Evropi i u Severnoj Americi, prisutna je i u susednim zemljama bivše Jugoslavije, Bosni i Hercegovini i Hrvatskoj. Prisustvo ove fitoplazme utvrđivano je primenom elektronske mikroskopije i molekularnom analizom uzoraka poreklom iz više lokaliteta u Srbiji, u periodu od 2004-2007 godine. Na ultratankim presecima floemskog tkiva glavnih lisnih nerava obolelih stabala kruške, utvrđeno je prisustvo organizama, koji po obliku, veličini (350-480 x 600-700nm) i strukturi, odgovaraju fitoplazmama. Rezultati PCR analiza, sa univerzalnim R16F2n/R16R2 i specifičnim prajmerom fO1/rO1 ukazali su na prisustvo fitoplazme Pear decline.
PB - Institute for Plant Protection and Environment, Belgrade
T2 - Zaštita bilja
T1 - The identification of the causal agent of the pear decline in Serbia
T1 - Identifikacija prouzrokovača propadanja kruške u Srbiji
EP - 67
IS - 1-4
SP - 57
VL - 57
ER -
@article{
author = "Starović, Mira and Ivanović, Žarko and Aleksić, Goran and Kuzmanović, Slobodan and Stojanović, Saša and Živković, Svetlana and Gavrilović, Veljko",
year = "2006",
abstract = "The symptoms pf pear decline are present in Serbia since 1970s. Thirty years later it was proved that the causal agent of this decline is a phytoplasma. This parasite is widespread in Europe and the USA as well as the countries of former Yugoslavia like Bosna and Hercegovina and Croatia. The presence of the phytoplasmas was confirmed using electron microscopy and the molecular analysis if the pear samples from a number of localities in Serbia between 2004-2007. The phytoplasma was observed on the ultrathin sections of the main leaf vascular bundle from the diseased pear tree. The shape, structure and size of 350-480 x 600-700nm, are typical ones for the phytoplasmas. PCR analysis using the universal primer R16F2n/R16R2 as well as the specific primer fO1/rO1 also confirmed phytoplasmas as the causal agent of pear decline., Simptomi propadanja kruške su prisutni u Srbiji od sedamdesetih godina prošlog veka. Mnogo godina kasnije, dokazano je da je prouzrokovač ovog propadanja fitoplazma kruške (PD). Ova pojava vrlo je raširena u Evropi i u Severnoj Americi, prisutna je i u susednim zemljama bivše Jugoslavije, Bosni i Hercegovini i Hrvatskoj. Prisustvo ove fitoplazme utvrđivano je primenom elektronske mikroskopije i molekularnom analizom uzoraka poreklom iz više lokaliteta u Srbiji, u periodu od 2004-2007 godine. Na ultratankim presecima floemskog tkiva glavnih lisnih nerava obolelih stabala kruške, utvrđeno je prisustvo organizama, koji po obliku, veličini (350-480 x 600-700nm) i strukturi, odgovaraju fitoplazmama. Rezultati PCR analiza, sa univerzalnim R16F2n/R16R2 i specifičnim prajmerom fO1/rO1 ukazali su na prisustvo fitoplazme Pear decline.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "The identification of the causal agent of the pear decline in Serbia, Identifikacija prouzrokovača propadanja kruške u Srbiji",
pages = "67-57",
number = "1-4",
volume = "57"
}
Starović, M., Ivanović, Ž., Aleksić, G., Kuzmanović, S., Stojanović, S., Živković, S.,& Gavrilović, V.. (2006). The identification of the causal agent of the pear decline in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 57(1-4), 57-67.
Starović M, Ivanović Ž, Aleksić G, Kuzmanović S, Stojanović S, Živković S, Gavrilović V. The identification of the causal agent of the pear decline in Serbia. in Zaštita bilja. 2006;57(1-4):57-67..
Starović, Mira, Ivanović, Žarko, Aleksić, Goran, Kuzmanović, Slobodan, Stojanović, Saša, Živković, Svetlana, Gavrilović, Veljko, "The identification of the causal agent of the pear decline in Serbia" in Zaštita bilja, 57, no. 1-4 (2006):57-67.
