Show simple item record

dc.creatorMartinović, Vesna I.
dc.creatorIvanović, Žarko
dc.creatorMihailović, Mirjana
dc.creatorIvanović-Matić, Svetlana K.
dc.creatorPoznanović, Goran D.
dc.creatorVidaković, Melita S.
dc.date.accessioned2019-04-04T16:02:15Z
dc.date.available2019-04-04T16:02:15Z
dc.date.issued2015
dc.identifier.issn0354-4664
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/389
dc.description.abstractWe examined the functions of the rat lymphocyte nuclear matrix after a single exposure to total body irradiation with doses ranging from sublethal to lethal. Irradiation induced systemic oxidative stress, detected as increased activities of serum SOD and catalase, lymphocyte DNA damage, detected by the Comet assay, and apoptosis. After irradiation with lower doses, the recruitment of DNA repair centers on the matrix was observed by Western analysis as increased levels of matrix-associated PARP-1, p53 and PCNA. Augmented partitioning of the pro-survival transcription factor NF-kappa B on the matrix was also detected after irradiation. Exposure to a lethal dose caused breakdown of the matrix, observed as lamin B cleavage, and of the matrix-associated DNA repair centers, detected as caspase-mediated PARP-1 proteolysis and loss of protein associations with the matrix. These findings suggest that the nuclear matrix establishes functional interactions in a defensive mechanism, integrated in a decision-making process that resolves cell fate.en
dc.publisherUniversity of Belgrade, University of Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS//
dc.rightsopenAccess
dc.sourceArchives of Biological Sciences
dc.subjectDNA repairen
dc.subjectionizing radiationen
dc.subjectNF-kappa Ben
dc.subjectnuclear matrixen
dc.subjectp53en
dc.subjectPARP-1en
dc.subjectPCNAen
dc.titleLymphocytes' 'last stand' on the nuclear matrix after whole body exposure of rats to low-let ionizing radiationen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage81
dc.citation.issue1
dc.citation.other67(1): 69-81
dc.citation.rankM23
dc.citation.spage69
dc.citation.volume67
dc.type.versionpublishedVersion
dc.identifier.doi10.2298/ABS140124002M
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1522/387.pdf
dc.identifier.rcubconv_902
dc.identifier.scopus2-s2.0-84937441539
dc.identifier.wos000352003500008


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record