Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue.
Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.
Keywords:
grapevine / viruses / ELISA / tissue blot / vinova loza / vizuelni test / ELISA test / 'tissue blot' test
Source:
Zaštita bilja, 2006, 57, 1-4, 69-79
Publisher:
Institute for Plant Protection and Environment, Belgrade
TY - JOUR
AU - Ivanović, Žarko
AU - Kuzmanović, Slobodan
AU - Trkulja, Nenad
AU - Živković, Svetlana
AU - Stojanović, Saša
AU - Starović, Mira
PY - 2006
UR - http://plantarum.izbis.bg.ac.rs/handle/123456789/40
AB - Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue.
AB - Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.
PB - Institute for Plant Protection and Environment, Belgrade
T2 - Zaštita bilja
T1 - DTBA and ELISA methods in detection of grapevine leafroll-1 virus
T1 - Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1
EP - 79
IS - 1-4
SP - 69
VL - 57
ER -
@article{
author = "Ivanović, Žarko and Kuzmanović, Slobodan and Trkulja, Nenad and Živković, Svetlana and Stojanović, Saša and Starović, Mira",
year = "2006",
url = "http://plantarum.izbis.bg.ac.rs/handle/123456789/40",
abstract = "Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue., Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "DTBA and ELISA methods in detection of grapevine leafroll-1 virus, Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1",
pages = "79-69",
number = "1-4",
volume = "57"
}
Ivanović, Ž., Kuzmanović, S., Trkulja, N., Živković, S., Stojanović, S.,& Starović, M. (2006). Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1.
Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 57(1-4), 69-79.
Ivanović Ž, Kuzmanović S, Trkulja N, Živković S, Stojanović S, Starović M. Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1. Zaštita bilja. 2006;57(1-4):69-79
Ivanović Žarko, Kuzmanović Slobodan, Trkulja Nenad, Živković Svetlana, Stojanović Saša, Starović Mira, "Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1" Zaštita bilja, 57, no. 1-4 (2006):69-79
