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DTBA and ELISA methods in detection of grapevine leafroll-1 virus

Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1

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2006
38.pdf (2.534Mb)
Authors
Ivanović, Žarko
Kuzmanović, Slobodan
Trkulja, Nenad
Živković, Svetlana
Stojanović, Saša
Starović, Mira
Article (Published version)
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Abstract
Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue.
Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.
Keywords:
grapevine / viruses / ELISA / tissue blot / vinova loza / vizuelni test / ELISA test / 'tissue blot' test
Source:
Zaštita bilja, 2006, 57, 1-4, 69-79
Publisher:
  • Institute for Plant Protection and Environment, Belgrade

ISSN: 0372-7866

[ Google Scholar ]
URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/40
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IZBIS
TY  - JOUR
AU  - Ivanović, Žarko
AU  - Kuzmanović, Slobodan
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Stojanović, Saša
AU  - Starović, Mira
PY  - 2006
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/40
AB  - Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue.
AB  - Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - DTBA and ELISA methods in detection of grapevine leafroll-1 virus
T1  - Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1
EP  - 79
IS  - 1-4
SP  - 69
VL  - 57
UR  - conv_187
ER  - 
@article{
author = "Ivanović, Žarko and Kuzmanović, Slobodan and Trkulja, Nenad and Živković, Svetlana and Stojanović, Saša and Starović, Mira",
year = "2006",
abstract = "Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue., Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "DTBA and ELISA methods in detection of grapevine leafroll-1 virus, Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1",
pages = "79-69",
number = "1-4",
volume = "57",
url = "conv_187"
}
Ivanović, Ž., Kuzmanović, S., Trkulja, N., Živković, S., Stojanović, S.,& Starović, M.. (2006). DTBA and ELISA methods in detection of grapevine leafroll-1 virus. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 57(1-4), 69-79.
conv_187
Ivanović Ž, Kuzmanović S, Trkulja N, Živković S, Stojanović S, Starović M. DTBA and ELISA methods in detection of grapevine leafroll-1 virus. in Zaštita bilja. 2006;57(1-4):69-79.
conv_187 .
Ivanović, Žarko, Kuzmanović, Slobodan, Trkulja, Nenad, Živković, Svetlana, Stojanović, Saša, Starović, Mira, "DTBA and ELISA methods in detection of grapevine leafroll-1 virus" in Zaštita bilja, 57, no. 1-4 (2006):69-79,
conv_187 .

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