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Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia

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Authors
Balaž, Jelica
Ivanović, Žarko
Davidović, Andrej
Ilicić, Renata
Janse, Jaap
Popović, Tatjana
Article (Published version)
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Abstract
Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates with colony morphology typical for the genus Xanthomonas were characterized with pathogenicity, biochemical, serological, and molecular assays. All 17 isolates reacted positive in a polymerase chain reaction (PCR) using XcpM1 and XcpM2 primers specific for Xanthomonas hortorum pv. pelargonii. In pathogenicity tests on Pelargonium zonale (leaf and stem inoculation), all isolates caused typical symptoms on leaves starting 2 days after inoculation as sunken, water-soaked, irregular lesions, and 6 to 8 days after inoculation on stems as necrotic lesions also showing yellow exudate. Symptoms resulted in general wilting of inoculated plants 20 days after inoculation. Selected phenotypic tests indicated that all isolates showed the same results as described for the bacterium X. hortorum pv. pelargonii. Repetitive sequence-based PCR typi...ng using BOX and ERIC revealed that all isolates showed two fingerprinting profiles but (GTG)(5) and REP did not reveal differences. Multilocus sequence typing of partial sequences of rpoD, dnaK, fyuA, and gyrB genes of tested isolates and sequences obtained from GenBank of Xanthomonas pathovar pathotype strains did not reveal genetic variability among the isolates, showing the same gene sequence pattern.

Source:
Plant Disease, 2016, 100, 1, 164-170
Publisher:
  • Amer Phytopathological Soc, St Paul
Funding / projects:
  • Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-43010)
  • Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control (RS-173026)

DOI: 10.1094/PDIS-03-15-0295-RE

ISSN: 0191-2917

PubMed: 30688579

WoS: 000368318200021

Scopus: 2-s2.0-84961365416
[ Google Scholar ]
2
2
URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/426
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IZBIS
TY  - JOUR
AU  - Balaž, Jelica
AU  - Ivanović, Žarko
AU  - Davidović, Andrej
AU  - Ilicić, Renata
AU  - Janse, Jaap
AU  - Popović, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/426
AB  - Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates with colony morphology typical for the genus Xanthomonas were characterized with pathogenicity, biochemical, serological, and molecular assays. All 17 isolates reacted positive in a polymerase chain reaction (PCR) using XcpM1 and XcpM2 primers specific for Xanthomonas hortorum pv. pelargonii. In pathogenicity tests on Pelargonium zonale (leaf and stem inoculation), all isolates caused typical symptoms on leaves starting 2 days after inoculation as sunken, water-soaked, irregular lesions, and 6 to 8 days after inoculation on stems as necrotic lesions also showing yellow exudate. Symptoms resulted in general wilting of inoculated plants 20 days after inoculation. Selected phenotypic tests indicated that all isolates showed the same results as described for the bacterium X. hortorum pv. pelargonii. Repetitive sequence-based PCR typing using BOX and ERIC revealed that all isolates showed two fingerprinting profiles but (GTG)(5) and REP did not reveal differences. Multilocus sequence typing of partial sequences of rpoD, dnaK, fyuA, and gyrB genes of tested isolates and sequences obtained from GenBank of Xanthomonas pathovar pathotype strains did not reveal genetic variability among the isolates, showing the same gene sequence pattern.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia
EP  - 170
IS  - 1
SP  - 164
VL  - 100
DO  - 10.1094/PDIS-03-15-0295-RE
ER  - 
@article{
author = "Balaž, Jelica and Ivanović, Žarko and Davidović, Andrej and Ilicić, Renata and Janse, Jaap and Popović, Tatjana",
year = "2016",
abstract = "Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates with colony morphology typical for the genus Xanthomonas were characterized with pathogenicity, biochemical, serological, and molecular assays. All 17 isolates reacted positive in a polymerase chain reaction (PCR) using XcpM1 and XcpM2 primers specific for Xanthomonas hortorum pv. pelargonii. In pathogenicity tests on Pelargonium zonale (leaf and stem inoculation), all isolates caused typical symptoms on leaves starting 2 days after inoculation as sunken, water-soaked, irregular lesions, and 6 to 8 days after inoculation on stems as necrotic lesions also showing yellow exudate. Symptoms resulted in general wilting of inoculated plants 20 days after inoculation. Selected phenotypic tests indicated that all isolates showed the same results as described for the bacterium X. hortorum pv. pelargonii. Repetitive sequence-based PCR typing using BOX and ERIC revealed that all isolates showed two fingerprinting profiles but (GTG)(5) and REP did not reveal differences. Multilocus sequence typing of partial sequences of rpoD, dnaK, fyuA, and gyrB genes of tested isolates and sequences obtained from GenBank of Xanthomonas pathovar pathotype strains did not reveal genetic variability among the isolates, showing the same gene sequence pattern.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia",
pages = "170-164",
number = "1",
volume = "100",
doi = "10.1094/PDIS-03-15-0295-RE"
}
Balaž, J., Ivanović, Ž., Davidović, A., Ilicić, R., Janse, J.,& Popović, T.. (2016). Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 164-170.
https://doi.org/10.1094/PDIS-03-15-0295-RE
Balaž J, Ivanović Ž, Davidović A, Ilicić R, Janse J, Popović T. Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia. in Plant Disease. 2016;100(1):164-170.
doi:10.1094/PDIS-03-15-0295-RE .
Balaž, Jelica, Ivanović, Žarko, Davidović, Andrej, Ilicić, Renata, Janse, Jaap, Popović, Tatjana, "Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia" in Plant Disease, 100, no. 1 (2016):164-170,
https://doi.org/10.1094/PDIS-03-15-0295-RE . .

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