'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors
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2016
Authors
Mitrović, Milana
Jakovljević, Miljana

Jović, Jelena

Krstić, Oliver

Kosovac, Andrea

Trivellone, Valeria
Jermini, Mauro
Toševski, Ivo

Cvrković, Tatjana

Article (Published version)

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A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-spec...ific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.
Keywords:
Hyalesthes obsoletus / Multilocus typing / Potato stolbur / Reptalus panzer / Reptalus quinquecostatus / VectorSource:
European Journal of Plant Pathology, 2016, 144, 3, 619-630Publisher:
- Springer, Dordrecht
Funding / projects:
- Swiss National Science Foundation - IZ73Z0_152414
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-43001)
DOI: 10.1007/s10658-015-0800-y
ISSN: 0929-1873
WoS: 000368998100013
Scopus: 2-s2.0-84955684642
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IZBISTY - JOUR AU - Mitrović, Milana AU - Jakovljević, Miljana AU - Jović, Jelena AU - Krstić, Oliver AU - Kosovac, Andrea AU - Trivellone, Valeria AU - Jermini, Mauro AU - Toševski, Ivo AU - Cvrković, Tatjana PY - 2016 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/428 AB - A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors. PB - Springer, Dordrecht T2 - European Journal of Plant Pathology T1 - 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors EP - 630 IS - 3 SP - 619 VL - 144 DO - 10.1007/s10658-015-0800-y ER -
@article{ author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Jermini, Mauro and Toševski, Ivo and Cvrković, Tatjana", year = "2016", abstract = "A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.", publisher = "Springer, Dordrecht", journal = "European Journal of Plant Pathology", title = "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors", pages = "630-619", number = "3", volume = "144", doi = "10.1007/s10658-015-0800-y" }
Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Jermini, M., Toševski, I.,& Cvrković, T.. (2016). 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology Springer, Dordrecht., 144(3), 619-630. https://doi.org/10.1007/s10658-015-0800-y
Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Jermini M, Toševski I, Cvrković T. 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology. 2016;144(3):619-630. doi:10.1007/s10658-015-0800-y .
Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Jermini, Mauro, Toševski, Ivo, Cvrković, Tatjana, "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors" in European Journal of Plant Pathology, 144, no. 3 (2016):619-630, https://doi.org/10.1007/s10658-015-0800-y . .