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Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification

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2016
443.pdf (771.8Kb)
Authors
Stepanović, Svetlana
Kosovac, Andrea
Krstić, Oliver
Jović, Jelena
Toševski, Ivo
Article (Published version)
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Abstract
Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwe...re applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.

Keywords:
Ceutorhynchus contractus / Ceutorhynchus erysimi / DNA barcoding / molecular identification / morphology / PCR-RFLP
Source:
Insect Science, 2016, 23, 4, 638-648
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-43001)

DOI: 10.1111/1744-7917.12212

ISSN: 1672-9609

PubMed: 25726808

WoS: 000384175700015

Scopus: 2-s2.0-85028261707
[ Google Scholar ]
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9
URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/445
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IZBIS
TY  - JOUR
AU  - Stepanović, Svetlana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/445
AB  - Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.
PB  - Wiley, Hoboken
T2  - Insect Science
T1  - Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification
EP  - 648
IS  - 4
SP  - 638
VL  - 23
DO  - 10.1111/1744-7917.12212
ER  - 
@article{
author = "Stepanović, Svetlana and Kosovac, Andrea and Krstić, Oliver and Jović, Jelena and Toševski, Ivo",
year = "2016",
abstract = "Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.",
publisher = "Wiley, Hoboken",
journal = "Insect Science",
title = "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification",
pages = "648-638",
number = "4",
volume = "23",
doi = "10.1111/1744-7917.12212"
}
Stepanović, S., Kosovac, A., Krstić, O., Jović, J.,& Toševski, I.. (2016). Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science
Wiley, Hoboken., 23(4), 638-648.
https://doi.org/10.1111/1744-7917.12212
Stepanović S, Kosovac A, Krstić O, Jović J, Toševski I. Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science. 2016;23(4):638-648.
doi:10.1111/1744-7917.12212 .
Stepanović, Svetlana, Kosovac, Andrea, Krstić, Oliver, Jović, Jelena, Toševski, Ivo, "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification" in Insect Science, 23, no. 4 (2016):638-648,
https://doi.org/10.1111/1744-7917.12212 . .

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