Prikaz osnovnih podataka o dokumentu

dc.creatorStepanović, Svetlana
dc.creatorKosovac, Andrea
dc.creatorKrstić, Oliver
dc.creatorJović, Jelena
dc.creatorToševski, Ivo
dc.date.accessioned2019-04-04T16:03:55Z
dc.date.available2019-04-04T16:03:55Z
dc.date.issued2016
dc.identifier.issn1672-9609
dc.identifier.urihttps://plantarum.izbis.bg.ac.rs/handle/123456789/445
dc.description.abstractGenotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.rightsopenAccess
dc.sourceInsect Science
dc.subjectCeutorhynchus contractusen
dc.subjectCeutorhynchus erysimien
dc.subjectDNA barcodingen
dc.subjectmolecular identificationen
dc.subjectmorphologyen
dc.subjectPCR-RFLPen
dc.titleMorphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identificationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage648
dc.citation.issue4
dc.citation.other23(4): 638-648
dc.citation.rankM21
dc.citation.spage638
dc.citation.volume23
dc.type.versionpublishedVersion
dc.identifier.doi10.1111/1744-7917.12212
dc.identifier.fulltexthttps://plantarum.izbis.bg.ac.rs//bitstream/id/1555/443.pdf
dc.identifier.pmid25726808
dc.identifier.scopus2-s2.0-85028261707
dc.identifier.wos000384175700015


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