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dc.creatorMitrović, Milana
dc.creatorTomanović, Željko
dc.creatorJakovljević, Miljana
dc.creatorRadović, D.
dc.creatorHavelka, J.
dc.creatorStary, Petr
dc.date.accessioned2019-04-04T16:03:55Z
dc.date.available2019-04-04T16:03:55Z
dc.date.issued2016
dc.identifier.issn0007-4853
dc.identifier.urihttps://plantarum.izbis.bg.ac.rs/handle/123456789/446
dc.description.abstractPopulations of Liparus glabrirostris (Curculionidae: Molytinae), a weevil inhabiting higher altitudes of Central Europe, were sampled from 24 localities in the Alps and Carpathian Mountains, and the geographical structuring of genetic variation was analyzed. Comparison of the concatenated mitochondrial cytochrome oxidase subunit I and subunit II sequences revealed consistent genetic divergence between the populations of L. glabrirostris from different mountain ranges. In phylogenetic analysis using maximum parsimony and median-joining networks, concatenated mitochondrial haplotypes from the Alps and Carpathians clustered as separate lineages, with high bootstrap support. Substantial genetic distances determined between the separated groups ranged from 2.6 to 3.0%, with divergence estimated to have initiated approximately 0.85-0.98 million years ago. The nuclear elongation factor 1 alpha gene was additionally amplified and haplotype analysis showed very low evolutionary divergence (0.2%), with separate clustering as well. The observed divergence suggests that the populations have been isolated for a long time, as a consequence of environmental changes resulting in varying fragmentation of habitats in the Alps and Carpathians, interrupting genetic exchange events and altering the genetic structure of L. glabrirostris populations. On the other hand, comparison of morphological characteristics showed no differences to confirm genetically well differentiated groups of populations. A polymerase chain reaction and restriction fragment length polymorphism-based method was therefore developed to discriminate between the Alpine and Carpathian lineages.en
dc.publisherCambridge Univ Press, Cambridge
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.rightsrestrictedAccess
dc.sourceBulletin of Entomological Research
dc.subjectLiparus glabrirostrisen
dc.subjectmitochondrial DNAen
dc.subjectnuclear elongation factor 1 alphaen
dc.subjectallopatric speciationen
dc.titleGenetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountainsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage662
dc.citation.issue5
dc.citation.other106(5): 651-662
dc.citation.rankM21
dc.citation.spage651
dc.citation.volume106
dc.type.versionpublishedVersion
dc.identifier.doi10.1017/S0007485316000377
dc.identifier.pmid27216121
dc.identifier.rcubconv_955
dc.identifier.scopus2-s2.0-84969961410
dc.identifier.wos000384238800011


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