The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN toge...ther with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.
TY - JOUR
AU - Ristić, Danijela
AU - Vučurović, Ivan
AU - Kuzmanović, Slobodan
AU - Milosević, Drago
AU - Gašić, Katarina
AU - Dolovac, Nenad
AU - Starović, Mira
PY - 2016
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/449
AB - The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.
PB - Serbian Genetics Society
T2 - Genetika-Belgrade
T1 - Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia
EP - 496
IS - 2
SP - 487
VL - 48
DO - 10.2298/GENSR1602487R
ER -
@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Milosević, Drago and Gašić, Katarina and Dolovac, Nenad and Starović, Mira",
year = "2016",
abstract = "The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia",
pages = "496-487",
number = "2",
volume = "48",
doi = "10.2298/GENSR1602487R"
}
Ristić, D., Vučurović, I., Kuzmanović, S., Milosević, D., Gašić, K., Dolovac, N.,& Starović, M.. (2016). Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 48(2), 487-496.
https://doi.org/10.2298/GENSR1602487R
Ristić D, Vučurović I, Kuzmanović S, Milosević D, Gašić K, Dolovac N, Starović M. Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade. 2016;48(2):487-496.
doi:10.2298/GENSR1602487R .
Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Milosević, Drago, Gašić, Katarina, Dolovac, Nenad, Starović, Mira, "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia" in Genetika-Belgrade, 48, no. 2 (2016):487-496,
https://doi.org/10.2298/GENSR1602487R . .
