Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia
2016
Authors
Pavlović, SnežanaRistić, Danijela
Vučurović, Ivan
Stevanović, Miloš
Stojanović, Sasa
Kuzmanović, Slobodan
Starović, Mira
Article (Published version)
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Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzc...hozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.
Keywords:
morphological characteristics / Ptmpmella anisum / PCR detection / seed infectionSource:
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2016, 44, 2, 411-417Publisher:
- Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca
Funding / projects:
- Development of integrated approach in plant protection for control harmful organisms (RS-MESTD-Technological Development (TD or TR)-31018)
DOI: 10.15835/nbha44210488
ISSN: 0255-965X
WoS: 000390832800012
Scopus: 2-s2.0-85007197680
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IZBISTY - JOUR AU - Pavlović, Snežana AU - Ristić, Danijela AU - Vučurović, Ivan AU - Stevanović, Miloš AU - Stojanović, Sasa AU - Kuzmanović, Slobodan AU - Starović, Mira PY - 2016 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/450 AB - Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation. PB - Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca T2 - Notulae Botanicae Horti Agrobotanici Cluj-Napoca T1 - Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia EP - 417 IS - 2 SP - 411 VL - 44 DO - 10.15835/nbha44210488 ER -
@article{ author = "Pavlović, Snežana and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš and Stojanović, Sasa and Kuzmanović, Slobodan and Starović, Mira", year = "2016", abstract = "Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.", publisher = "Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca", journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca", title = "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia", pages = "417-411", number = "2", volume = "44", doi = "10.15835/nbha44210488" }
Pavlović, S., Ristić, D., Vučurović, I., Stevanović, M., Stojanović, S., Kuzmanović, S.,& Starović, M.. (2016). Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca., 44(2), 411-417. https://doi.org/10.15835/nbha44210488
Pavlović S, Ristić D, Vučurović I, Stevanović M, Stojanović S, Kuzmanović S, Starović M. Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2016;44(2):411-417. doi:10.15835/nbha44210488 .
Pavlović, Snežana, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, Stojanović, Sasa, Kuzmanović, Slobodan, Starović, Mira, "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44, no. 2 (2016):411-417, https://doi.org/10.15835/nbha44210488 . .