Study of the anatomical structure of Chenopodium album leaves in relation to susceptibility to herbicides

Abstract

As the plant matures numerous changes at the morphological, physiological and anatomical level occur, leading to changes in the susceptibility to herbicides. Anatomical characteristics of apical fully developed leaves of Chenopodium album were studied in this paper. From plants at 2, 4, 6, 8 and 10 leaves stage, fully developed leaves were sampled and used to make permanent slides for light microscopy. The following parameters were measured on permanent microscope slide samples: thickness of the leaf, epidermal adaxial and abaxial, thickness of the mesophyll, palisade and spongy tissue. The obtained results have shown that maturing of the C. album plants can affect the anatomical structure of its fully developed leaves. Hence, it has been confirmed that the tissue thickness in leaves collected from plants in younger stages of development is significantly different from the tissue thickness of corresponding leaves collected from older plants.

Sa starenjem biljke najčešće dolazi do promena na morfološkom, fiziološkom i anatomskom nivou, što dovodi do promena u osetljivosti na herbicide. Ova istraživanja obavljena su sa ciljem da se utvrdi da li se sa starenjem biljaka menjaju anatomske karakteristike gornjih fiziološki zrelih listova Chenopodium album. Sa biljaka koje su bile u fazi 2, 4, 6, 8 i 10 listova uzorkovani su listovi u punoj fiziološkoj zrelosti i napravljeni trajni preparati za svetlosnu mikroskopiju, na kojima su mereni: debljina lista, debljina epidermalnih ćelija lica i naličja lista, debljina mezofila, palisadnog i sunđerastog tkiva. Dobijeni rezultati su pokazali da starenje vrste C. album može uticati na anatomsku građu fiziološki zrelih listova. Potvrđeno je da se debljina tkiva listova uzetih sa mlađih biljaka značajno razlikuje od debljine istih listova uzetih sa starijih biljaka.