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dc.creatorTrkulja, Nenad
dc.creatorMilosavljević, Anja
dc.creatorMitrović, Milana
dc.creatorJović, Jelena
dc.creatorToševski, Ivo
dc.creatorKhan, Mohamed F. R.
dc.creatorSecor, Gary A.
dc.date.accessioned2019-04-04T16:04:46Z
dc.date.available2019-04-04T16:04:46Z
dc.date.issued2017
dc.identifier.issn0929-1873
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/475
dc.description.abstractCercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.en
dc.publisherSpringer, Dordrecht
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31018/RS//
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Plant Pathology
dc.subjectMulti-resistanceen
dc.subjectCercospora beticolaen
dc.subjectMolecular characterizationen
dc.subjectCYP51en
dc.subjectbeta-tubulinen
dc.subjectCoben
dc.titleMolecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicidesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage910
dc.citation.issue4
dc.citation.other149(4): 895-910
dc.citation.rankM21
dc.citation.spage895
dc.citation.volume149
dc.type.versionpublishedVersion
dc.identifier.doi10.1007/s10658-017-1239-0
dc.identifier.rcubconv_985
dc.identifier.scopus2-s2.0-85018794747
dc.identifier.wos000414691500011


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